Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-mRFP1"

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<div id="wrap">
 
<div id="wrap">
 +
 
<div class="imageimage1">
 
<div class="imageimage1">
 
<div class="wordword1">
 
<div class="wordword1">
  
−
<h1>TITLE</h1><hr />
+
<div class="satisfy">
−
<p>Besides the fungal killing switch and the functional prototype that help reduce concerns over GMO</p>
+
<h1>mRFP1</h1></div>
 +
<div class="satisfysp">
 +
<h1>mRFP1</h1></div>
 +
<hr />
  
−
</div></div></div>
+
</div></div><br /></div>
  
 
<div class="expand">
 
<div class="expand">
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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/9</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">BBa_E1010 T-streaked onto agar plates</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">Week 2: (1-7 July)</div>
+
<div class="technology">7/10</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>The ordered primers were taken this week.</p>
+
<li><p style="font-size: 18px;">BBa_E1010 extracted the checked with restriction digestion (EocRI and PstI,   747bp and 2029bp)</p></li>
−
<p>We would produce our biobricks, but the result was not like we expected after electrophoresis. Then we made colony PCR  and again do it for SOD and GPX. After this, we did digestion and also pTRE vector .</p>
+
−
<img src="https://static.igem.org/mediawiki/2014/7/7e/ATOMS-Notebook1.tif.png">
+
−
<img src="https://static.igem.org/mediawiki/2014/1/17/ATOMS-Notebook2.png">
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/16</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/17</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/18</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">BBa_E1010 checked with plasmid PCR with newly design primers</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
 
+
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
+
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/19</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">BBa_E1010 checked with plasmid PCR with the addition of Mg2+</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/22</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">BBa_E1010 checked with Taq polymerase plasmid PCR (successful)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/13</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 PCR amplification</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<img src="" width=""100% />
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
 
+
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/16</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 digestion</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/19</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 PCR amplification (failed)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/21</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 PCR amplification</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 digestion</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/25</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 checked with PCR due to multiple failed double ligation</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/26</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 checked with KOD polymerase PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">mRFP1 amplified with KOD polymerase PCR</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/5</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">mRFP1 amplified with KOD polymerase PCR</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">9/9</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">mRFP1 amplified with KOD polymerase PCR</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 14); return false">[Collapse]</a></p>
 
</div>
 
</div>
  

Latest revision as of 10:24, 19 October 2016

mRFP1

mRFP1


[Collapse all] | [Expand all]

7/9

  • BBa_E1010 T-streaked onto agar plates

    • [Collapse]

    7/10

  • BBa_E1010 extracted the checked with restriction digestion (EocRI and PstI, 747bp and 2029bp)

    • [Collapse]

    7/16

  • BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)

    • [Collapse]

    7/17

  • BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)

    • [Collapse]

    7/18

  • BBa_E1010 checked with plasmid PCR with newly design primers

    • 7/19

  • BBa_E1010 checked with plasmid PCR with the addition of Mg2+

    • [Collapse]

    7/22

  • BBa_E1010 checked with Taq polymerase plasmid PCR (successful)

    • [Collapse]

    8/13

  • mRFP1 PCR amplification

    • [Collapse]

    8/16

  • mRFP1 digestion

    • [Collapse]

    8/19

  • mRFP1 PCR amplification (failed)

    • [Collapse]

    8/21

  • mRFP1 PCR amplification

  • mRFP1 digestion

    • [Collapse]

    8/25

  • mRFP1 checked with PCR due to multiple failed double ligation

    • [Collapse]

    8/26

  • mRFP1 checked with KOD polymerase PCR

  • mRFP1 amplified with KOD polymerase PCR

    • [Collapse]

    9/5

  • mRFP1 amplified with KOD polymerase PCR

    • [Collapse]

    9/9

  • mRFP1 amplified with KOD polymerase PCR

    • [Collapse]