Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-TtrpC"
| Line 153: | Line 153: | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">VP-TR trouble shooting</p></li> | <li><p style="font-size: 18px;">VP-TR trouble shooting</p></li> | ||
| − | <li><p style="font-size: 18px;">VP-TR PCR amplification</p></li> | + | <li><p style="font-size: 18px;">VP-TR PCR amplification(correct)</p></li> |
| + | <img src="" width="100%" /> | ||
| + | <p style="font-size: 18px;">Target sequence: TtrpC :791 base pairs</p> | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p> | ||
| Line 201: | Line 203: | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">9/11</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">TtrpC KOD PCR amplification</p></li> | <li><p style="font-size: 18px;">TtrpC KOD PCR amplification</p></li> | ||
| Line 209: | Line 211: | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">9/19</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">Amplified TtrpC form plasmid pAN7-1</p></li> | <li><p style="font-size: 18px;">Amplified TtrpC form plasmid pAN7-1</p></li> | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p> | ||
| + | </div> | ||
| + | |||
| + | <div class="technology">10/1</div> | ||
| + | <div class="thelanguage"> | ||
| + | <li><p style="font-size: 18px;">TtrpC digestion (for ligation with pBARGPE1)</p></li> | ||
| + | |||
| + | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
Revision as of 10:50, 19 October 2016
TtrpC
TtrpC
7/22
Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station
pAN7-1 transformation
pAN7-1 did 2 in 1
7/23
pAN7-1 plasmid extraction
7/24
pAN7-1 amplified with plasmid PCR (primer: minPgpdA)
pAN7-1 extracted and transformed cultures cryopreserved
7/29
VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)
7/31
VP-TR trouble shooting
VP-TR PCR amplification(correct)
Target sequence: TtrpC :791 base pairs
8/13
TtrpC PCR amplification for VP- EL222_RFP
8/15
TtrpC and pVP- EL222 PCR amplificaiton
8/16
TtrpC digestion
8/23
8/26
TtrpC KOD PCR check(with remixed pVP- EL222 primers)
8/27
TtrpC KOD PCR amplification
9/11
9/19
Amplified TtrpC form plasmid pAN7-1
10/1
TtrpC digestion (for ligation with pBARGPE1)
