Revision as of 11:35, 19 October 2016

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Andersen Promoters

Relative Strength: weak (0.33) BBa_J23110

Selected as a relatively weak promoter against which to compare the expression of the PETase construct

Relative Strength: moderate (0.58) BBa_J23111

Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct

Relative Strength: strong (1) BBa_J23100

Selected as a relatively strong promoter against which to compare the expression of the PETase construct

N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of e. Coli

C-Myc tag: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression

PETase sequence: gene that coded for the PET hydrolase

Plasmid backbone: the pSB1C3 backbone is recommended

High efficiency e. Coli cells: hosted the PETase constructs

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            <a class="dropdown-toggle" data-toggle="dropdown" href="">Project<span class="caret"></span></a>
            <ul class="dropdown-menu">
-             <li><a href="https://2016.igem.org/ASIJProjectDescription">Project Description + Abstract</a></li>
+             <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/ASIJProjectDescription">Project Description + Abstract</a></li>
              <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Experiments">Experiments</a></li>
              <li><a href="https://2016.igem.org/Team:ASIJ_Tokyo/Results">Results</a></li>