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Revision as of 11:54, 19 October 2016

index

Type

Function

Length

dszA (a monooxygenase gene )

coding

convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway

1383 bp

dszB (a desulfinase gene

coding

convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway

1117 bp

dszC (a monooxygenase gene )

coding

not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway

1274 bp

dszD (a flavinreductase)

coding

three successive oxidation steps require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) during the 4S pathway

599 bp

dszA (a monooxygenase gene ), E.coli-optimized

coding

convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway

1383 bp

dszB (a desulfinase gene), E.coli-optimized

coding

convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway

1170 bp

dszC (a monooxygenase gene ), E.coli-optimized

coding

not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway

1274 bp

dszBCAD with T7 promoter

coding

dszBCAD genes with T7 promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

4537 bp

T7 RNA polymerase induced by IPTG

coding

T7 RNA polymerase will be expressed with IPTG added.

4552 bp

dszBCAD with Lac promoter

coding

dszBCAD genes with Lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

5859 bp

dszBAC with tac promoter and dszD with lac promoter

coding

dszBAC genes with tac promoter and dszD gene with lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

6052 bp

dszBCAD with T7 promoter induced by IPTG

composite

When IPTG added, dszBCAD genes with T7 promoter will be expressed greatly  which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)

9097 bp

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