(→Data recovery Bordeaux 2016) |
(→Data recovery Bordeaux 2016) |
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Here are those results: | Here are those results: | ||
+ | Using a surface slide system (SSS) the dynamics of the plasmid R1 and its permanently derepressed mutant Rldvdl9 in surface cultures of Escherichia coli K12 was examined. | ||
+ | |||
+ | The experiments were carried on E.coli K12 strain J53 hosting the plasmid R1 or it's premanently repressed mutant R1drd19. those two strains were used as donor cells. The recipient used is E.coli CH50. | ||
+ | |||
+ | {| class="wikitable" | ||
+ | | Surface mating JS3(RI) x CSHSO | ||
+ | |- | ||
+ | | Initial||Recipient||Donor||Transconjugant||Total | ||
+ | |- | ||
+ | | 10^7||3,8*10^8||1,5*10^8||2,1*10^5||5,2*10^8 | ||
+ | |- | ||
+ | | 10^6||3,1*10^8||1,8*10^8||2,1*10^5||4,8*10^8 | ||
+ | |- | ||
+ | | 10^5||2,2*10^8||1,8*10^8||6,5*10^4||4,1*10^8 | ||
+ | |- | ||
+ | | 10^4||2,7*10^8||1,9*10^8||3,0*10^4||4,6*10^8 | ||
+ | |- | ||
+ | | 10^3||2,7*10^8||1,9*10^8||3,0*10^4||4,6*10^8 | ||
+ | |- | ||
+ | | 10^2||2,9*10^8||2,2*10^8||1,6*10^4||5,0*10^8 | ||
+ | |- | ||
+ | | |||||||| | ||
+ | |- | ||
+ | | Surface mating J53(Rldrd19) x CSHSO|||||||| | ||
+ | |- | ||
+ | | Initial||Recipient||Donor||Transconjugant||Total | ||
+ | |- | ||
+ | | 10^7||6,6*10^7||1,7*10^8||9,4*10^7||3,3*10^8 | ||
+ | |- | ||
+ | | 10^6||1,4*10^8||2,5*10^8||4,4*10^7||4,4*10^8 | ||
+ | |- | ||
+ | | 10^5||1,4*10^8||1,9*10^8||8,0*10^6||3,4*10^8 | ||
+ | |- | ||
+ | | 10^4||1,6*10^8||2,3*10^8||1,8*10^6||3,9*10^8 | ||
+ | |- | ||
+ | | 10^3||2,3*10^8||2,6*10^8||4,7*10^5||4,9*10^8 | ||
+ | |- | ||
+ | | 10^2||2,4*10^8||2,2*10^8||4,1*10^4||4,7*10^8 | ||
+ | |- | ||
+ | | |||||||| | ||
+ | |- | ||
+ | | Liquid mating JS3(RI) x CSHSO|||||||| | ||
+ | |- | ||
+ | | Initial||Recipient||Donor||Transconjugant||Total | ||
+ | |- | ||
+ | | 10^7||2,2*10^8||9,8*10^7||4,4*10^3||3,1*10^8 | ||
+ | |- | ||
+ | | 10^6||1,7*10^8||8,5*10^7||5,9*10^3||2,6*10^8 | ||
+ | |- | ||
+ | | 10^5||2,0*10^8||6,9*10^7||7,9*10^3||2,7*10^8 | ||
+ | |- | ||
+ | | 10^4||2,7*10^8||6,1*10^7||1,6*10^4||2,7*10^8 | ||
+ | |- | ||
+ | | 10^3||2,9*10^8||4,9*10^7||1,9*10^4||2,9*10^8 | ||
+ | |- | ||
+ | | 10^2||4,1*10^8||1,0*10^7||6,0*10^3||4,1*10^8 | ||
+ | |- | ||
+ | | |||||||| | ||
+ | |- | ||
+ | | Liquid mating JS3(Rldrd19) x CSHSO|||||||| | ||
+ | |- | ||
+ | | Initial||Recipient||Donor||Transconjugant||Total | ||
+ | |- | ||
+ | | 10^7||1,2*10^8||6,8*10^7||7,4*10^7||2,6*10^8 | ||
+ | |- | ||
+ | | 10^6||1,7*10^8||3,7*10^7||8,4*10^7||2,9*10^8 | ||
+ | |- | ||
+ | | 10^5||1,7*10^8||2,7*10^7||7,4*10^7||2,7*10^8 | ||
+ | |- | ||
+ | | 10^4||1,9*10^8||1,8*10^7||6,6*10^7||2,7*10^8 | ||
+ | |- | ||
+ | | 10^3||2,3*10^8||7,4*10^6||4,7*10^7||2,8*10^8 | ||
+ | |- | ||
+ | | 10^2||2,6*10^8||3,7*10^6||1,2*10^7||2,8*10^8 | ||
+ | |- | ||
+ | | | ||
+ | |} | ||
<references/> | <references/> |