<p id="pp">A serial dilution of FITC provided in the interkab kit was prepared using PBS. Fluorescence of all samples was measured in standard measurement modes. Measurements were repeated to produce a series of standard curves. The fluorescence readings were taken at 26.4°C at 477 nm to 515 nm excitation and emission respectively. Gain setting measured at 46,56,66. A setting change of 10 units makes a significant difference to the fluorescence data. The plate reader was run at optimum gain setting. This set gain to 76, which was too high for these measurements as the weakest dilution had a lower fluorescence than the pure PBS. Ran the reader at 37°C and 56 gain to produce our optimal standard curve.</p>

<p id="pp">A serial dilution of FITC provided in the interkab kit was prepared using PBS. Fluorescence of all samples was measured in standard measurement modes. Measurements were repeated to produce a series of standard curves. The fluorescence readings were taken at 26.4°C at 477 nm to 515 nm excitation and emission respectively. Gain setting measured at 46,56,66. A setting change of 10 units makes a significant difference to the fluorescence data. The plate reader was run at optimum gain setting. This set gain to 76, which was too high for these measurements as the weakest dilution had a lower fluorescence than the pure PBS. Ran the reader at 37°C and 56 gain to produce our optimal standard curve.</p>

<p id="pp">Competent cells of E.coli DH5α were prepared following the provided protocol. Tested the OD of the cells using standard settings (see iGEM 2016 file),aiiming for a reading of 0.4-0.5. The first reading produced an average of 0.2, after 15 minutes the average increased to 2.5. The cells were spun down and re-suspended in TF-1 and TF-2 buffer. 100µl was aliquoted into 1 ml Eppendorf tubes and immediately dropped into liquid nitrogen. The cells were then left in the -80°C freezer. Chloramphenicol was made as a stock of 25mg/ml (weight measured was 76.6mg) and added to LB media to make plates for the iGEM interlab parts transformed with different constructs.</p>

<p id="pp">Competent cells of E.coli DH5α were prepared following the provided protocol. Tested the OD of the cells using standard settings (see iGEM 2016 file),aiiming for a reading of 0.4-0.5. The first reading produced an average of 0.2, after 15 minutes the average increased to 2.5. The cells were spun down and re-suspended in TF-1 and TF-2 buffer. 100µl was aliquoted into 1 ml Eppendorf tubes and immediately dropped into liquid nitrogen. The cells were then left in the -80°C freezer. Chloramphenicol was made as a stock of 25mg/ml (weight measured was 76.6mg) and added to LB media to make plates for the iGEM interlab parts transformed with different constructs.</p>