Difference between revisions of "Team:Imperial College/Composite Part"

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<specialh3> Best Composite Part - pBAD-Gp2 </specialh3>
  
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<p>This year our best composite part is the pBAD-Gp2 construct. It provides a new way to repress growth in bacteria without killing the host cell. We have demonstrated that the cell is able to recover its initial growth rate once Gp2 is not expressed and can recover back to a normal growth rate. We are excited to provide this new growth regulator for the synthetic biology community and for the future iGEM teams.
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<specialh4>Key Advantages</specialh4>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>→The protein is very small, and can inhibit growth within a half-hour timeframe. <br>
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→The repression is robust and reversible.<br>
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→The system does not require any toxins or supplements in the media.<br>
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→Usage of the gene avoids the problem of antimicrobial resistance. <br><br>
 
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<img src="https://static.igem.org/mediawiki/2016/1/19/T--Imperial_College--composite_part.png" />
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<p><b>Figure 1:</b> Schematic of the pBAD-Gp2 construct </p>
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<p><br><br>For our best composite part, we have selected the pBAD-Gp2 part. It was created by cloning a synthesised sequence block that contained Gp2, an RBS and the biobrick prefix and suffix, into an pBAD construct created by the 2014 Imperial iGEM team. <br><br>
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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The construct effectively and rapidly downregulates the growth of E. coli in response to arabinose, and does so in a reversible manner. We believe it is a good alternative to existing growth control methods, especially for controlling co-culture, for several reasons<br><br>
  
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→Controlling cells in this way doesn’t require the usage of supplemented or minimal media, as in the case of antibiotics and auxotrophy.<br>
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→The gene is already naturally in the environment within the E.coli virus T7 phage, so there aren’t major biocontainment issues or the problems associated with antimicrobial resistance.<br>
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→It is specific to E. coli, and so, while possibly toxic to other prokaryotic bacteria, will not have any effect on eukaryotes. <br>
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→Gp2 is particularly good for regulating population size in co-cultures, as it only affects the size of one population.<br><br>
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<img src="https://static.igem.org/mediawiki/2016/4/4c/T--Imperial_College--Gp2.png" />
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<p><b>Figure 1:</b> Schematic of the pBAD-Gp2 construct <br><br> </p>
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<h4>Note</h4>
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<img src="https://static.igem.org/mediawiki/2016/d/de/T--Imperial_College--Gp2_repression_%281%29.png" />
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<p><b>Figure 1:</b> Schematic of the pBAD-Gp2 construct </p>
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Revision as of 12:39, 19 October 2016

Best Composite Part - pBAD-Gp2

This year our best composite part is the pBAD-Gp2 construct. It provides a new way to repress growth in bacteria without killing the host cell. We have demonstrated that the cell is able to recover its initial growth rate once Gp2 is not expressed and can recover back to a normal growth rate. We are excited to provide this new growth regulator for the synthetic biology community and for the future iGEM teams.

Key Advantages

→The protein is very small, and can inhibit growth within a half-hour timeframe.
→The repression is robust and reversible.
→The system does not require any toxins or supplements in the media.
→Usage of the gene avoids the problem of antimicrobial resistance.

Figure 1: Schematic of the pBAD-Gp2 construct



For our best composite part, we have selected the pBAD-Gp2 part. It was created by cloning a synthesised sequence block that contained Gp2, an RBS and the biobrick prefix and suffix, into an pBAD construct created by the 2014 Imperial iGEM team.

The construct effectively and rapidly downregulates the growth of E. coli in response to arabinose, and does so in a reversible manner. We believe it is a good alternative to existing growth control methods, especially for controlling co-culture, for several reasons

→Controlling cells in this way doesn’t require the usage of supplemented or minimal media, as in the case of antibiotics and auxotrophy.
→The gene is already naturally in the environment within the E.coli virus T7 phage, so there aren’t major biocontainment issues or the problems associated with antimicrobial resistance.
→It is specific to E. coli, and so, while possibly toxic to other prokaryotic bacteria, will not have any effect on eukaryotes.
→Gp2 is particularly good for regulating population size in co-cultures, as it only affects the size of one population.

Figure 1: Schematic of the pBAD-Gp2 construct

Figure 1: Schematic of the pBAD-Gp2 construct