Difference between revisions of "Team:Exeter/Interlab"
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<p id="pp">The InterLab measurement kit contained 5 devices; (1) J23101+I13504, (2) J23106+I13504, (3) J23117+I13504, Positive and Negative control. The first attempt to transform 5µL of each device into our competent cells found no liquid in the tubes provided. The provided tubes were re-suspended using elution buffer from a Qiagen mini-prep kit. A transformation of plasmids used in the lab under a strong promoter and GFP was performed mirroring the Interpab protocol to determine if the cells were competent. Spread plates were made and incubated at 37°C.</p> | <p id="pp">The InterLab measurement kit contained 5 devices; (1) J23101+I13504, (2) J23106+I13504, (3) J23117+I13504, Positive and Negative control. The first attempt to transform 5µL of each device into our competent cells found no liquid in the tubes provided. The provided tubes were re-suspended using elution buffer from a Qiagen mini-prep kit. A transformation of plasmids used in the lab under a strong promoter and GFP was performed mirroring the Interpab protocol to determine if the cells were competent. Spread plates were made and incubated at 37°C.</p> | ||
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<p id="pp">Competent cells containing the previously used strong promoter and GFP coding sequence showed strong expression of GFP. However the competent cells transformed with the iGEM parts showed no growth. A new interlab kit was ordered from iGEM.</p> | <p id="pp">Competent cells containing the previously used strong promoter and GFP coding sequence showed strong expression of GFP. However the competent cells transformed with the iGEM parts showed no growth. A new interlab kit was ordered from iGEM.</p> | ||
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<p id="pp">The measurements were taken using the same calibration settings as before on the Tecan plate reader every hour for 6 hours. The plate was placed into mini shaking incubator at 37°C between plate readings. A scratch was noticed on the plate lid and was replaced. This lid was cold compared to the incubated plate and so condensation formed this could have affected the results. Placed in incubator briefly to warm up.</p> | <p id="pp">The measurements were taken using the same calibration settings as before on the Tecan plate reader every hour for 6 hours. The plate was placed into mini shaking incubator at 37°C between plate readings. A scratch was noticed on the plate lid and was replaced. This lid was cold compared to the incubated plate and so condensation formed this could have affected the results. Placed in incubator briefly to warm up.</p> | ||
| + | <div class="row"> | ||
| + | <div class="col-xs-6" style="padding:5px 2% 5px 10%;margin:0;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/1/1b/T--Exeter--interlabgraphforpart1egl.png" | ||
| + | style="max-width:100%;margin:auto;display:block;"> | ||
| + | <span class="caption">Fig.</span> | ||
| + | <br> | ||
| + | <br> | ||
| + | </div> | ||
| + | <div class="col-xs-6" style="padding:5px 10% 5px 2%;margin:0;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/a/a6/T--Exeter--latinrectangleinterlabgraphforpart1egl.png" | ||
| + | style="max-width:100%;margin:auto;display:block;"> | ||
| + | <span class="caption">Fig.</span> | ||
| + | <br> | ||
| + | <br> | ||
| + | </div> | ||
| + | </div> | ||
Revision as of 13:02, 19 October 2016
