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<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<h3>Golden Gate MoClo<h3> | <h3>Golden Gate MoClo<h3> | ||
− | <p class="text-faded" style="font-size: 15px"> | + | <p class="text-faded" style="font-size: 15px">Central to synthetic biology is the use of systematic assembly and sharing of parts between community members. As working in new chassis will emphasizes on characterisation of suitable parts and trial-error approach, we chose to adopt Golden Gate MoClo as our assembly standard. This approach enables the assembly of multiple parts and transcriptional units by parallel approach via BpiI (BbsI) in different levels (Engler et al., 2008; Weber et al., 2011). |
− | + | <br><br>To utilise MoClo, a set of destination vectors need to be developed to accommodate assembly in different levels. Unfortunately, the chassis we were working on (R. jostii) cannot utilise the origin of replication from available destination vectors, which are designed for Eschericia coli. Therefore, we developed a set of MoClo destination vectors for assembly and transformation to R. jostii. We have shown that our destination vectors can be used for combinatorial assembly and transformed to the host organism. | |
+ | </p> | ||
</div> | </div> | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
<h3>Developed Parts (Phytobricks)<h3> | <h3>Developed Parts (Phytobricks)<h3> | ||
− | <p class="text-faded" style="font-size: 15px"> | + | <p class="text-faded" style="font-size: 15px">Central to synthetic biology is the use of systematic assembly and sharing of parts between community members. As working in new chassis will emphasizes on characterisation of suitable parts and trial-error approach, we chose to adopt Golden Gate MoClo as our assembly standard. This approach enables the assembly of multiple parts and transcriptional units by parallel approach via BpiI (BbsI) in different levels (Engler et al., 2008; Weber et al., 2011). |
+ | <br><br>To utilise MoClo, a set of destination vectors need to be developed to accommodate assembly in different levels. Unfortunately, the chassis we were working on (R. jostii) cannot utilise the origin of replication from available destination vectors, which are designed for Eschericia coli. Therefore, we developed a set of MoClo destination vectors for assembly and transformation to R. jostii. We have shown that our destination vectors can be used for combinatorial assembly and transformed to the host organism. | ||
</p> | </p> | ||
</div> | </div> |
Revision as of 13:25, 19 October 2016
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