Difference between revisions of "Team:Aix-Marseille/Medals"

(→‎Silver medal)
(→‎Bronze medal)
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===Bronze medal===
 
===Bronze medal===
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We have successfully registered for iGEM and we had a great summer. We will attends the Giant Jamboree and we are ready!!!
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All the deliverables have been met.
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Our Attribution page is created and all the work done during this fabulous project is referenced [https://2016.igem.org/Team:Aix-Marseille/Attributions here].
  
 
[[File:T--Aix-Marseille--Despatway2.png|300px|left|thumb|DesA in Desferrioxamine B biosynthesis patway]]
 
[[File:T--Aix-Marseille--Despatway2.png|300px|left|thumb|DesA in Desferrioxamine B biosynthesis patway]]
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To compete for the bronze medal, we have set up the '''[http://parts.igem.org/Part:BBa_K1951000 BBa_K1951000] simple biobrick'''. We have designed this biobrick from the sequence of ''desA'' of ''Streptomyces coelicolor'' A3(2) (complete genome [https://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3 here]). It codes for the lysine descarboxylase DesA. It is an enzyme from the lyase family that converts lysine to cadaverine. ''desA'' is the first gene from the ''des'' operon. This operon is involved in biosynthesis of the Desferrioxamine B, a bacterial siderophore using to bind ferric ion from the environment.
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We have set up the '''[http://parts.igem.org/Part:BBa_K1951000 BBa_K1951000] simple biobrick'''. We have designed this biobrick from the sequence of ''desA'' of ''Streptomyces coelicolor'' A3(2) (complete genome [https://www.ncbi.nlm.nih.gov/nuccore/NC_003888.3 here]). It codes for the lysine descarboxylase DesA. It is an enzyme from the lyase family that converts lysine to cadaverine. ''desA'' is the first gene from the ''des'' operon. This operon is involved in biosynthesis of the Desferrioxamine B, a bacterial siderophore using to bind ferric ion from the environment.
 
As this sequence comes from a gram positive bacterium (i.e., ''Streptomyces coelicolor'' A3(2)), we obtimised codon for expression in ''Escherichia coli'' by using codon optimization IDT software<ref> https://eu.idtdna.com/CodonOpt </ref>.
 
As this sequence comes from a gram positive bacterium (i.e., ''Streptomyces coelicolor'' A3(2)), we obtimised codon for expression in ''Escherichia coli'' by using codon optimization IDT software<ref> https://eu.idtdna.com/CodonOpt </ref>.
  

Revision as of 14:20, 19 October 2016