Medals
Which medals do we compete for ?
Bronze medal
DesA in Desferrioxamine B biosynthesis patway
We have set up the [http://parts.igem.org/Part:BBa_K1951000 BBa_K1951000] simple biobrick. We have designed this biobrick from the sequence of desA of Streptomyces coelicolor A3(2) (complete genome here). It codes for the lysine descarboxylase DesA. It is an enzyme from the lyase family that converts lysine to cadaverine. desA is the first gene from the des operon. This operon is involved in biosynthesis of the Desferrioxamine B, a bacterial siderophore using to bind ferric ion from the environment.
As this sequence comes from a gram positive bacterium (i.e., Streptomyces coelicolor A3(2)), we obtimised codon for expression in Escherichia coli by using codon optimization IDT software[1].
We have successfully registered for iGEM and we had a great summer. We will attends the Giant Jamboree and we are ready!!!
All the deliverables have been met.
Our Attribution page is created and all the work done during this fabulous project is referenced here.
Silver medal
To compete for the silver medal, we have set up the [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004] functionnal composite biobrick. This biobrick were designed from [http://parts.igem.org/Part:BBa_I0500 I0500] (pARA/araC inducible promoter), [http://parts.igem.org/Part:BBa_B0034 B0034] (Ribosome Binding Site) and our designed biobrick [http://parts.igem.org/Part:BBa_K1951000 K1951000] (Lysine decarboxylase DesA).
To test its functionnality, we have complemented the cadA mutant with our biobrick [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004]. Results have shown a recovery of the cadaverine biosynthesis and even beyond the rate obtained with the WT strain. To more information, please visit this [http://parts.igem.org/Part:BBa_K1951004:Experience page].
We also help other iGEM teams with their project by reading a paper or making a model of plasmid loss. All of this can be found here.
For our human practices, we present the iGEM competition and the use of the synthetic biology in many events.
Gold medal
To reach the gold medal, we made a big work to integrate our human practices to our project. You can find all this work right here.
We also improve an existing BioBrick ([parts.igem.org/Part:BBa_K1951008 BBa K1951008]). This BioBrick is an improve of the Glasgow 2014 team's BioBrick ([http://parts.igem.org/Part:BBa_K1463604 BBa K1463604]) Our BioBrick is optimized to have a strong expression in E.coli (thanks to the codon usage), it RBS has no mutation so the flagellin is well expressed and is functionnal and our swimming test show that the swimming phenotype of the BioBick is improved thanks to our changes. All the functionnal tests and experiments are listed here.
