Difference between revisions of "Team:TU Darmstadt/Notebook"
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<div class="labWeek"><h5>Lab Week 22: <div class="week_date"> September 12 - September 18</div></h5></div></td> | <div class="labWeek"><h5>Lab Week 22: <div class="week_date"> September 12 - September 18</div></h5></div></td> | ||
<td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/b/b5/T--TU_Darmstadt--iconLab3.png"/></td> | <td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/b/b5/T--TU_Darmstadt--iconLab3.png"/></td> | ||
| − | <td><p> | + | <td><p><h6>Metabolic burden</h6> |
| + | |||
| + | <b>BBa_K1976000:</b><br> | ||
| + | The BioBrick BBa_E0240 was cut with the restriction enzymes <i>XbaI</i> and <i>PstI</i>.<br> | ||
| + | BBa_J61002 was cut with <i>SpeI</i> and <i>PstI</i>.<br> | ||
| + | The two restriction products were ligated by standard T4‑Ligation protocol. <br> | ||
| + | |||
| + | <b>BBa_K1976001:</b><br> | ||
| + | B0034‑BBa_I11020 was cut with <i>XbaI</i> and <i>PstI</i>.<br> | ||
| + | BBa_J23101 was cut with <i>SpeI</i> and <i>PstI</i>.<br> | ||
| + | Both fragments were ligated using the T4‑Ligase by standard protocol.</p> | ||
</td></tr> | </td></tr> | ||
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td> | <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td> | ||
Revision as of 15:06, 19 October 2016
NOTEBOOK
Labjournal 2016
For half a year we were working in the wet lab 
, developed a pipetting robot 
and simulated the molecular dynamics of our proteins 
.
As well we would like to highlight certain social events 
which helped us to further improve our team spirit.
Lab Week 1: |
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Hier könnte Ihr labjournal stehen! |
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robotics | |
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modeling | |
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social |
Lab Week 2: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 3: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 4: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 5: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 6: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 7: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 8: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 9: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 10: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 11: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 12: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 13: |
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Hier könnte Ihr labjournal stehen! |
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social |
Lab Week 14: |
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Hier könnte Ihr labjournal stehen! |
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modeling | |
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Lab Week 15: |
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Hier könnte Ihr labjournal stehen! |
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robotics | |
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modeling | |
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social |
Lab Week 16: |
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Hier könnte Ihr labjournal stehen! |
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robotics | |
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modeling | |
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social |
Lab Week 17: |
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Hier könnte Ihr labjournal stehen! |
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robotics | |
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modeling | |
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social |
Lab Week 18: |
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Hier könnte Ihr labjournal stehen! |
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robotics | |
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modeling | |
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social |
Lab Week 19: |
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Hier könnte Ihr labjournal stehen! |
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robotics | |
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modeling | |
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social |
Lab Week 20: |
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Hier könnte Ihr labjournal stehen! |
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robotics | |
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modeling | |
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social |
Lab Week 21: |
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Hier könnte Ihr labjournal stehen! |
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Lab Week 22: |
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Metabolic burdenBBa_K1976000:The BioBrick BBa_E0240 was cut with the restriction enzymes XbaI and PstI. BBa_J61002 was cut with SpeI and PstI. The two restriction products were ligated by standard T4‑Ligation protocol. BBa_K1976001: B0034‑BBa_I11020 was cut with XbaI and PstI. BBa_J23101 was cut with SpeI and PstI. Both fragments were ligated using the T4‑Ligase by standard protocol. |
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robotics | |
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modeling | |
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social |
Lab Week 23: |
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Metabolic burdenBBa_K1976000:After plasmid preparation, the plasmid was cut with XbaI and PstI. We had the attp‑site BBa_11023 flanked by the two additional bidirectional transcription terminators BBa_1001 synthesized by IDT. The attp‑site of BBa_11023 was not the original λ‑attp‑site. Instead the sequence corresponded to the attP2‑site used in the Gateway ® cloning system. The construct was cut with EcoRI and SpeI. The pSB1C3 backbone was cut with EcoRI and PstI. Together with the previously cut BBa_J61002‑BBa_E0240 construct the synthesized BBa_1001‑BBa_11023‑BBa_1001 construct was ligated in the cut pSB1C3. BBa_K1976001: The ligation product was transformed into E. coli Top 10 via heatshock. |
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social |
Lab Week 24: |
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Metabolic burdenBBa_K1976000:The attP2‑site was mutated to λ‑attp via Quick‑Change‑PCR, using the following primers: QC_attp_fw and QC_attp_rev (for more details see our primerlist). BBa_K1976001: To check whether BBa_I11020 was expressed properly a SDS‑Page was made. |
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social |
Lab Week 25: |
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Metabolic burdenBBa_K1976000:The LVA‑tag was added by PCR with the overhang primer LVA_GFP_fw and LVA_GFP_rev. |
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modeling | |
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social |
Lab Week 26: |
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Metabolic burdenBBa_K1976000:For plasmid curing the final plasmid construct and the low copy backbone pSB4A5 were cut with EcoRI and PstI and then ligated. BBa_K1976001: For plasmid curing the final plasmid construct and the low copy vector pSB4K5 were cut with EcoRI and PstI and then ligated. BBa_K1976000 and BBa_K1976001 were cotransformed into E. coli Top 10. To check whether the genomic integration was successful a cPCR with attb_fw and VR primers was made. |
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modeling | |
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social |
Lab Week 27: |
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Metabolic burdenPlasmid curing could not be performed due to lack of time. |
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modeling | |
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social |
