Difference between revisions of "Team:Toronto/Parts"
| (3 intermediate revisions by the same user not shown) | |||
| Line 6: | Line 6: | ||
<!-- repo for this wiki: https://github.com/igemuoftATG/wiki2016 --> | <!-- repo for this wiki: https://github.com/igemuoftATG/wiki2016 --> | ||
| − | |||
| − | |||
</html> | </html> | ||
{{Toronto/head}} | {{Toronto/head}} | ||
| Line 64: | Line 62: | ||
</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li> | ||
| − | |||
| − | |||
</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | ||
| Line 81: | Line 77: | ||
<div class="content"> | <div class="content"> | ||
<div class="content" id="content-main"><div class="row"><div class="col col-lg-8 col-md-12"><div class="content-main"><h1 id="design">Design</h1> | <div class="content" id="content-main"><div class="row"><div class="col col-lg-8 col-md-12"><div class="content-main"><h1 id="design">Design</h1> | ||
| − | <h3 id="gols"> | + | <p><img src="https://static.igem.org/mediawiki/parts/2/29/Trial2016Toronto.jpeg" alt="alt text"></p> |
| − | <p>The wild type gene for the salmonella | + | <center><em>Figure 1: Designed Circuit</em></center> |
| − | <h3 id="gols-p118a"> | + | |
| − | <p>The | + | <h3 id="gols">GolS</h3> |
| + | <p>The wild type gene for the salmonella GolS protein. GolS functions by binding to an operator (pGolb)to repress transcription by bending the DNA. In the presence of gold ions, the repressor undergoes a conformational change and unbends the DNA. This allows for transcription of the operon genes. In salmonella, GolS is used as a response protein to regulate gold resistance protein expression.</p> | ||
| + | <h3 id="gols-p118a">GolS P118A</h3> | ||
| + | <p>The GolS repressor protein with a modified binding site to increase selectivity for gold ions and decrease interaction between copper ions. This modification ensures that products will only be responsive to one element. </p> | ||
<h3 id="mcherry">mCherry</h3> | <h3 id="mcherry">mCherry</h3> | ||
<p>A gene for a cherry coloured protein placed adjacent to pGolb to serve as a reporter for gold presence. </p> | <p>A gene for a cherry coloured protein placed adjacent to pGolb to serve as a reporter for gold presence. </p> | ||
<h3 id="teto-promoter">TetO Promoter</h3> | <h3 id="teto-promoter">TetO Promoter</h3> | ||
| − | <p>A strong constitutive promoter to express the | + | <p>A strong constitutive promoter to express the GolS protein, ensuring saturation of all pGolb sites with GolS so there’s no leak of reporter. </p> |
<h3 id="lacz">LacZ</h3> | <h3 id="lacz">LacZ</h3> | ||
<p>Alternative reporter protein used next to pGolb to indicate gold ion presence.</p> | <p>Alternative reporter protein used next to pGolb to indicate gold ion presence.</p> | ||
| Line 132: | Line 131: | ||
<p>Part Table</p> | <p>Part Table</p> | ||
</div></div><div id="tableofcontents" class="tableofcontents affix sidebar col-lg-4 hidden-xs hidden-sm hidden-md visible-lg-3"><ul class="nav"> | </div></div><div id="tableofcontents" class="tableofcontents affix sidebar col-lg-4 hidden-xs hidden-sm hidden-md visible-lg-3"><ul class="nav"> | ||
| − | <li><a href="#gols"> | + | <li><a href="#gols">GolS</a></li> |
| − | <li><a href="#gols-p118a"> | + | <li><a href="#gols-p118a">GolS P118A</a></li> |
<li><a href="#mcherry">mCherry</a></li> | <li><a href="#mcherry">mCherry</a></li> | ||
<li><a href="#teto-promoter">TetO Promoter</a></li> | <li><a href="#teto-promoter">TetO Promoter</a></li> | ||
Latest revision as of 15:10, 19 October 2016
Design
GolS
The wild type gene for the salmonella GolS protein. GolS functions by binding to an operator (pGolb)to repress transcription by bending the DNA. In the presence of gold ions, the repressor undergoes a conformational change and unbends the DNA. This allows for transcription of the operon genes. In salmonella, GolS is used as a response protein to regulate gold resistance protein expression.
GolS P118A
The GolS repressor protein with a modified binding site to increase selectivity for gold ions and decrease interaction between copper ions. This modification ensures that products will only be responsive to one element.
mCherry
A gene for a cherry coloured protein placed adjacent to pGolb to serve as a reporter for gold presence.
TetO Promoter
A strong constitutive promoter to express the GolS protein, ensuring saturation of all pGolb sites with GolS so there’s no leak of reporter.
LacZ
Alternative reporter protein used next to pGolb to indicate gold ion presence.
Parts Submitted
Part Table
