Difference between revisions of "Team:Toronto/Parts"

 
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</li>
 
</li>
 
<li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li>
 
<li><a href="https://2016.igem.org/Team:Toronto/HP-Gold"><span>gold</span></a></li>
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</li>
 
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<li><a href="https://2016.igem.org/Team:Toronto/HP-Impact"><span>impact</span></a></li>
 
 
</li>
 
</li>
 
<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li>
 
<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li>
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<div class="content">
 
<div class="content">
 
<div class="content" id="content-main"><div class="row"><div class="col col-lg-8 col-md-12"><div class="content-main"><h1 id="design">Design</h1>
 
<div class="content" id="content-main"><div class="row"><div class="col col-lg-8 col-md-12"><div class="content-main"><h1 id="design">Design</h1>
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<h3 id="gols">Gols</h3>
+
<p><img src="https://static.igem.org/mediawiki/parts/2/29/Trial2016Toronto.jpeg" alt="alt text"></p>
−
<p>The wild type gene for the salmonella Gols protein. Gols functions by binding to an operator (pGolb)to repress transcription by bending the DNA. In the presence of gold ions, the repressor undergoes a conformational change and unbends the DNA. This allows for transcription of the operon genes. In salmonella, Gols is used as a response protein to regulate gold resistance protein expression.</p>
+
<center><em>Figure 1: Designed Circuit</em></center>
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<h3 id="gols-p118a">Gols P118A</h3>
+
 
−
<p>The Gols repressor protein with a modified binding site to increase selectivity for gold ions and decrease interaction between copper ions.  This modification ensures that products will only be responsive to one element. </p>
+
<h3 id="gols">GolS</h3>
 +
<p>The wild type gene for the salmonella GolS protein. GolS functions by binding to an operator (pGolb)to repress transcription by bending the DNA. In the presence of gold ions, the repressor undergoes a conformational change and unbends the DNA. This allows for transcription of the operon genes. In salmonella, GolS is used as a response protein to regulate gold resistance protein expression.</p>
 +
<h3 id="gols-p118a">GolS P118A</h3>
 +
<p>The GolS repressor protein with a modified binding site to increase selectivity for gold ions and decrease interaction between copper ions.  This modification ensures that products will only be responsive to one element. </p>
 
<h3 id="mcherry">mCherry</h3>
 
<h3 id="mcherry">mCherry</h3>
 
<p>A gene for a cherry coloured protein placed adjacent to pGolb to serve as a reporter for gold presence. </p>
 
<p>A gene for a cherry coloured protein placed adjacent to pGolb to serve as a reporter for gold presence. </p>
 
<h3 id="teto-promoter">TetO Promoter</h3>
 
<h3 id="teto-promoter">TetO Promoter</h3>
−
<p>A strong constitutive promoter to express the Gols protein, ensuring saturation of all pGolb sites with Gols so there’s no leak of reporter. </p>
+
<p>A strong constitutive promoter to express the GolS protein, ensuring saturation of all pGolb sites with GolS so there’s no leak of reporter. </p>
 
<h3 id="lacz">LacZ</h3>
 
<h3 id="lacz">LacZ</h3>
 
<p>Alternative reporter protein used next to pGolb to indicate gold ion presence.</p>
 
<p>Alternative reporter protein used next to pGolb to indicate gold ion presence.</p>
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<p>Part Table</p>
 
<p>Part Table</p>
 
</div></div><div id="tableofcontents" class="tableofcontents affix sidebar col-lg-4 hidden-xs hidden-sm hidden-md visible-lg-3"><ul class="nav">
 
</div></div><div id="tableofcontents" class="tableofcontents affix sidebar col-lg-4 hidden-xs hidden-sm hidden-md visible-lg-3"><ul class="nav">
−
<li><a href="#gols">Gols</a></li>
+
<li><a href="#gols">GolS</a></li>
−
<li><a href="#gols-p118a">Gols P118A</a></li>
+
<li><a href="#gols-p118a">GolS P118A</a></li>
 
<li><a href="#mcherry">mCherry</a></li>
 
<li><a href="#mcherry">mCherry</a></li>
 
<li><a href="#teto-promoter">TetO Promoter</a></li>
 
<li><a href="#teto-promoter">TetO Promoter</a></li>

Latest revision as of 15:10, 19 October 2016

Design

alt text

Figure 1: Designed Circuit

GolS

The wild type gene for the salmonella GolS protein. GolS functions by binding to an operator (pGolb)to repress transcription by bending the DNA. In the presence of gold ions, the repressor undergoes a conformational change and unbends the DNA. This allows for transcription of the operon genes. In salmonella, GolS is used as a response protein to regulate gold resistance protein expression.

GolS P118A

The GolS repressor protein with a modified binding site to increase selectivity for gold ions and decrease interaction between copper ions. This modification ensures that products will only be responsive to one element.

mCherry

A gene for a cherry coloured protein placed adjacent to pGolb to serve as a reporter for gold presence.

TetO Promoter

A strong constitutive promoter to express the GolS protein, ensuring saturation of all pGolb sites with GolS so there’s no leak of reporter.

LacZ

Alternative reporter protein used next to pGolb to indicate gold ion presence.

Parts Submitted

Part Table

<groupparts>iGEM016 Toronto</groupparts>