Difference between revisions of "Team:Toronto/Notebook-w16-tue"
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<li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | <li><a href="https://2016.igem.org/Team:Toronto/Integrated_Practices"><span>integrated_practices</span></a></li> | ||
Latest revision as of 15:14, 19 October 2016
console.js:26
Tuesday - August 30, 2016
Tuesday, 8/30
Members Present: Bohdan, Kat, Celine, Hamed, Karim, Marc.
LAB:
Morning:
A | B | C | D | E | F | G | H | I | |
1 | C1 | P2 | R2 | G2 | SG1 | SP2 | |||
2 | Miniprep Concentration | 17.9 | 13.9 | 20.2 | 34.6 | 44.5 | 44.5 | ||
3 | Nuclease-free water | 0 | 0 | 0 | 5.44 | 8.01 | 0.12 | ||
4 | DNA | 17 | 17 | 17 | 11.56 | 8.99 | 16.88 | ||
5 | Cutsmart | 2 | 2 | 2 | 2 | 2 | 2 | ||
6 | Enzyme | 1 | 1 | 1 | 1 | 1 | 1 | ||
7 | Total volume | 20ul | 20ul | 20ul | 20ul | 20ul | 20ul | ||
8 | Final DNA Conc. | 15.215 | 15.81 | 17.17 | 20 | 20 | 20 | ||
Table1
Afternoon:
●
Gel extracted the the remainder of the constructs
●
RE digested the extracted constructs
●
Ran a gel on the extracted constructs:
●
Made O/N cultures of the constructs that were unsuccessfully extracted.
●
Transformed with miniprepped plasmid DNA from the stock initial assembled constructs.
Administrative:
TO DO:
For the next day:
LAB TEAM:
LAB MANAGERS:
Gmail correspondence:
Meetings/Notes:
References:
