Difference between revisions of "Team:NJU-China/Notebook/Calendar"
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<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 1 (05/22/2016-05/28/2016)</h4> |
| − | <p> | + | <p>Learn laboratory safety regulations and lab skills like PCR, restriction enzyme digestion, electrophoresis, fragment ligation, etc.</p> |
| + | <p>Acquire iRGD-Lamp2b fusion protein sequence from GenScript, then try to construct plasmid (pcDNA6.2) ---Failed</p> | ||
</div> | </div> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week 2 (05/29/2016-06/04/2016)</h4> |
| − | <p> | + | <p>PCR, Plasmid construction using iRGD-Lamp2b sequence and pSB1C3 backbone.---Failed</p> |
| + | <p>Plasmid construction using iRGD-Lamp2b sequence and pSB1C3 backbone.---Failed</p> | ||
| + | <p>Learn cellular experiment skills. (cell culture & transfection)</p> | ||
| + | <p>HEK293, A549-Luc, A549 cell lines recovery.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 112: | Line 116: | ||
</div> | </div> | ||
<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 3 (06/05/2016-06/11/2016)</h4> |
| − | <p> | + | <p>Plasmid construction using iRGD-Lamp2b sequence and pSB1C3 backbone.---Succeed</p> |
| + | <p>Learn western blot protocols.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 121: | Line 126: | ||
</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week 4 (06/12/2016-06/18/2016)</h4> |
| − | <p> | + | <p>Test software written by team SYSU-Software . Design siRNAs.</p> |
| + | <p>Order siRNA from GenScript.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 130: | Line 136: | ||
</div> | </div> | ||
<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 5 (06/19/2016-06/25/2016)</h4> |
| − | <p> | + | <p>Receive siRNA from GenScript, Test RNA interference efficiency by cell transfection and western blotting.--- Failed</p> |
| + | <p>Test KRAS expression level in which cell line using transfection and western blotting.---Succeed</p> | ||
| + | <p>Abandon cells contaminated with bacteria. Disinfection and sterilization in cell incubators.</p> | ||
| + | <p>Learn protocols for FCFM, ultra-high-speed centrifuge, in-vivo imaging.</p> | ||
| + | <p>Obtain access for State Key Laboratory of Pharmaceutical Biotechnology.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 139: | Line 149: | ||
</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week 6 (06/26/2016-07/02/2016)</h4> |
| − | <p> | + | <p>A549-Luc cell line recovery and culture. Preparation for animal modeling.</p> |
| + | <p>Design shRNA based on validated siRNA sequence. Send our designed sequence to GenScript for synthesis.</p> | ||
| + | <p>Successfully loading our exosomes with iRGD and siRNA for the first time. Send exosome samples to National University of Defense Technology for electron microscopy assay and Nanosight.</p> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 148: | Line 160: | ||
</div> | </div> | ||
<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 7 (07/03/2016-07/09/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week 8 (07/10/2016-07/16/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 9 (07/17/2016-07/23/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week 10 (07/24/2016-07/30/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 11 (07/31/2016-08/06/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week 12 (08/07/2016-08/13/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 13 (08/14/2016-08/20/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week 14 (08/21/2016-08/27/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week 15 (08/28/2016-09/03/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week16 (09/04/2016-09/10/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week17 (09/11/2016-09/17/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="timeline-content right"> | <div class="timeline-content right"> | ||
| − | <h4> | + | <h4>Week18 (09/18/2016-09/24/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
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<div class="timeline-content"> | <div class="timeline-content"> | ||
| − | <h4> | + | <h4>Week19 (09/25/2016-10/01/2016)</h4> |
<p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p> | ||
</div> | </div> | ||
Revision as of 15:45, 19 October 2016
Week 1 (05/22/2016-05/28/2016)
Learn laboratory safety regulations and lab skills like PCR, restriction enzyme digestion, electrophoresis, fragment ligation, etc.
Acquire iRGD-Lamp2b fusion protein sequence from GenScript, then try to construct plasmid (pcDNA6.2) ---Failed
Week 2 (05/29/2016-06/04/2016)
PCR, Plasmid construction using iRGD-Lamp2b sequence and pSB1C3 backbone.---Failed
Plasmid construction using iRGD-Lamp2b sequence and pSB1C3 backbone.---Failed
Learn cellular experiment skills. (cell culture & transfection)
HEK293, A549-Luc, A549 cell lines recovery.
Week 3 (06/05/2016-06/11/2016)
Plasmid construction using iRGD-Lamp2b sequence and pSB1C3 backbone.---Succeed
Learn western blot protocols.
Week 4 (06/12/2016-06/18/2016)
Test software written by team SYSU-Software . Design siRNAs.
Order siRNA from GenScript.
Week 5 (06/19/2016-06/25/2016)
Receive siRNA from GenScript, Test RNA interference efficiency by cell transfection and western blotting.--- Failed
Test KRAS expression level in which cell line using transfection and western blotting.---Succeed
Abandon cells contaminated with bacteria. Disinfection and sterilization in cell incubators.
Learn protocols for FCFM, ultra-high-speed centrifuge, in-vivo imaging.
Obtain access for State Key Laboratory of Pharmaceutical Biotechnology.
Week 6 (06/26/2016-07/02/2016)
A549-Luc cell line recovery and culture. Preparation for animal modeling.
Design shRNA based on validated siRNA sequence. Send our designed sequence to GenScript for synthesis.
Successfully loading our exosomes with iRGD and siRNA for the first time. Send exosome samples to National University of Defense Technology for electron microscopy assay and Nanosight.
Week 7 (07/03/2016-07/09/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 8 (07/10/2016-07/16/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 9 (07/17/2016-07/23/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 10 (07/24/2016-07/30/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 11 (07/31/2016-08/06/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 12 (08/07/2016-08/13/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 13 (08/14/2016-08/20/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 14 (08/21/2016-08/27/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week 15 (08/28/2016-09/03/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week16 (09/04/2016-09/10/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week17 (09/11/2016-09/17/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week18 (09/18/2016-09/24/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
Week19 (09/25/2016-10/01/2016)
Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.
