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| | margin-left: auto; | | margin-left: auto; |
| | margin-right: auto; | | margin-right: auto; |
| | + | list-style-type: disc; |
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| | div.thelanguage img { | | div.thelanguage img { |
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| | <div id="wrap"> | | <div id="wrap"> |
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| | <div class="imageimage1"> | | <div class="imageimage1"> |
| | <div class="wordword1"> | | <div class="wordword1"> |
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| − | <h1>TITLE</h1><hr /> | + | <div class="satisfy"> |
| − | <p>Besides the fungal killing switch and the functional prototype that help reduce concerns over GMO</p> | + | <h1>C120 empty</h1></div> |
| | + | <div class="satisfysp"> |
| | + | <h1>C120 empty</h1></div> |
| | + | <hr /> |
| | | | |
| − | </div></div></div> | + | </div></div><br /></div> |
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| | <div class="expand"> | | <div class="expand"> |
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| | style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important; /*margin: 12px !important;*/ font-size: 18px !important; text-decoration: none;">[Expand all]</a></p></center></div> | | style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important; /*margin: 12px !important;*/ font-size: 18px !important; text-decoration: none;">[Expand all]</a></p></center></div> |
| | | | |
| − | <div class="technology">transformation efficiency </div> | + | <div class="technology">7/4</div> |
| | <div class="thelanguage"> | | <div class="thelanguage"> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p> | + | < li><p style="font-size: 18px;"> Received plasmid C120_empty from University of Texas Southwestern</p></ li> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| | | | |
| | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> |
| | </div> | | </div> |
| | | | |
| − | <div class="technology">Week 2: (1-7 July)</div> | + | <div class="technology">7/13</div> |
| | <div class="thelanguage"> | | <div class="thelanguage"> |
| − | <p>The ordered primers were taken this week.</p> | + | < li><p style="font-size: 18px;"> pC120_empty resuspended (sample from University of Texas Southwestern)< /p></ li> |
| − | <p>We would produce our biobricks, but the result was not like we expected after electrophoresis. Then we made colony PCR and again do it for SOD and GPX. After this, we did digestion and also pTRE vector .</p> | + | <li><p style="font-size: 18px;">pC120_empty transformation attempted (failed, no cultures on first plate)</p></li> |
| − | <img src="https://static.igem.org/mediawiki/2014/7/7e/ATOMS-Notebook1.tif.png"> | + | |
| − | <img src="https://static.igem.org/mediawiki/2014/1/17/ATOMS-Notebook2.png"> | + | |
| | | | |
| | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p> | | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p> |
| | </div> | | </div> |
| | | | |
| − | <div class="technology">transformation efficiency </div> | + | <div class="technology">7/14</div> |
| | <div class="thelanguage"> | | <div class="thelanguage"> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p> | + | < li><p style="font-size: 18px;"> pC120_empty transformation</p></ li> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| | | | |
| | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p> | | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p> |
| | </div> | | </div> |
| | | | |
| − | <div class="technology">transformation efficiency </div> | + | <div class="technology">7/15</div> |
| | <div class="thelanguage"> | | <div class="thelanguage"> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p> | + | < li><p style="font-size: 18px;"> pC120_empty extracted from transformed cultures and checked with PstI restriction digestion</p></ li> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| | | | |
| | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p> | | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p> |
| | </div> | | </div> |
| | | | |
| − | <div class="technology">transformation efficiency </div> | + | <div class="technology">7/16</div> |
| | <div class="thelanguage"> | | <div class="thelanguage"> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p> | + | < li><p style="font-size: 18px;"> pC120_empty plasmid stored and transformed cultures cryopreserved</ p></ li> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | | + | |
| − | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p> | + | |
| | </div> | | </div> |
| | | | |
| − | <div class="technology">transformation efficiency </div> | + | <div class="technology">7/28</div> |
| | <div class="thelanguage"> | | <div class="thelanguage"> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p> | + | < li><p style="font-size: 18px;"> pC120_empty ran restriction digestion for the ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)</p></ li> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| | | | |
| | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> |
| | </div> | | </div> |
| | | | |
| − | <div class="technology">transformation efficiency </div> | + | <div class="technology">7/30</div> |
| | <div class="thelanguage"> | | <div class="thelanguage"> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p> | + | < li><p style="font-size: 18px;"> pC120_empty digestion for the ligation with HindIII and BamHI</p></ li> |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| + | |
| | | | |
| | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> | | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> |
| − | </div>
| |
| − |
| |
| − | <div class="technology">transformation efficiency </div>
| |
| − | <div class="thelanguage">
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − |
| |
| − | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="technology">transformation efficiency </div>
| |
| − | <div class="thelanguage">
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − |
| |
| − | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="technology">transformation efficiency </div>
| |
| − | <div class="thelanguage">
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − |
| |
| − | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="technology">transformation efficiency </div>
| |
| − | <div class="thelanguage">
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − |
| |
| − | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
| |
| − | </div>
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| − |
| |
| − | <div class="technology">transformation efficiency </div>
| |
| − | <div class="thelanguage">
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
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| − |
| |
| − | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
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| − | </div>
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| − |
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| − | <div class="technology">transformation efficiency </div>
| |
| − | <div class="thelanguage">
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
| |
| − | <p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
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| − |
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| − | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
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| | </div> | | </div> |
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