Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-C120 empty"

 
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<div class="technology">7/4</div>
 
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<li><p style="font-size: 18px;">Received plasmid C120_empty from university of Texas Southwestern</p></li>
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<li><p style="font-size: 18px;">Received plasmid C120_empty from University of Texas Southwestern</p></li>
  
 
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<div class="technology">7/28</div>
 
<div class="technology">7/28</div>
 
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<li><p style="font-size: 18px;">pC120_empty ran restriction digestion for ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)</p></li>
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<li><p style="font-size: 18px;">pC120_empty ran restriction digestion for the ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)</p></li>
  
 
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<div class="technology">7/30</div>
 
<div class="technology">7/30</div>
 
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<li><p style="font-size: 18px;">pC120_empty digestion for ligation with HindIII and BamHI</p></li>
+
<li><p style="font-size: 18px;">pC120_empty digestion for the ligation with HindIII and BamHI</p></li>
  
 
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Latest revision as of 16:55, 19 October 2016

C120 empty

C120 empty



7/4
  • Received plasmid C120_empty from University of Texas Southwestern

  • [Collapse]

    7/13
  • pC120_empty resuspended (sample from University of Texas Southwestern)

  • pC120_empty transformation attempted (failed, no cultures on first plate)

  • [Collapse]

    7/14
  • pC120_empty transformation

  • [Collapse]

    7/15
  • pC120_empty extracted from transformed cultures and checked with PstI restriction digestion

  • [Collapse]

    7/16
  • pC120_empty plasmid stored and transformed cultures cryopreserved

  • 7/28
  • pC120_empty ran restriction digestion for the ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)

  • [Collapse]

    7/30
  • pC120_empty digestion for the ligation with HindIII and BamHI

  • [Collapse]