Line 121: | Line 121: | ||
<div class="technology">7/4</div> | <div class="technology">7/4</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
− | <li><p style="font-size: 18px;">Received plasmid C120_empty from | + | <li><p style="font-size: 18px;">Received plasmid C120_empty from University of Texas Southwestern</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | ||
Line 155: | Line 155: | ||
<div class="technology">7/28</div> | <div class="technology">7/28</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
− | <li><p style="font-size: 18px;">pC120_empty ran restriction digestion for ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)</p></li> | + | <li><p style="font-size: 18px;">pC120_empty ran restriction digestion for the ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | ||
Line 162: | Line 162: | ||
<div class="technology">7/30</div> | <div class="technology">7/30</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
− | <li><p style="font-size: 18px;">pC120_empty digestion for ligation with HindIII and BamHI</p></li> | + | <li><p style="font-size: 18px;">pC120_empty digestion for the ligation with HindIII and BamHI</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> |
Latest revision as of 16:55, 19 October 2016
C120 empty
C120 empty
7/4
Received plasmid C120_empty from University of Texas Southwestern
7/13
pC120_empty resuspended (sample from University of Texas Southwestern)
pC120_empty transformation attempted (failed, no cultures on first plate)
7/14
pC120_empty transformation
7/15
pC120_empty extracted from transformed cultures and checked with PstI restriction digestion
7/16
pC120_empty plasmid stored and transformed cultures cryopreserved
7/28
pC120_empty ran restriction digestion for the ligation with HindIII and BamHI (pC120_empty concentration was too low and contamination of the samples were detected)
7/30
pC120_empty digestion for the ligation with HindIII and BamHI