Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-RFP"
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<div id="wrap"> | <div id="wrap"> | ||
| + | |||
<div class="imageimage1"> | <div class="imageimage1"> | ||
<div class="wordword1"> | <div class="wordword1"> | ||
| − | <h1> | + | <div class="satisfy"> |
| − | < | + | <h1>RFP</h1></div> |
| + | <div class="satisfysp"> | ||
| + | <h1>RFP</h1></div> | ||
| + | <hr /> | ||
| − | </div></div></div> | + | </div></div><br /></div> |
<div class="expand"> | <div class="expand"> | ||
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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important; /*margin: 12px !important;*/ font-size: 18px !important; text-decoration: none;">[Expand all]</a></p></center></div> | style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important; /*margin: 12px !important;*/ font-size: 18px !important; text-decoration: none;">[Expand all]</a></p></center></div> | ||
| − | <div class="technology"> | + | <div class="technology">7/9</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 T-streaked onto agar plates</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/10</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 extracted plasmid and checked with restriction digestion (EocRI and PstI 747bp and 2029bp)</p></li> |
| − | + | ||
| − | < | + | |
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/16</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/17</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/18</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 checked with plasmid PCR with newly design primers</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/19</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 checked with plasmid PCR with the addition of Mg2+</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/22</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 checked with Taq polymerase plasmid PCR (successful)</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/25</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">BBa_E1010 amplified with KOD polymerase PCR</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/26</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <p> | + | <li><p style="font-size: 18px;">RFP amplified with KOD polymerase PCR</p></li> |
| − | + | <li><p style="font-size: 18px;">RFP digested with EcoRI and BamHI</p></li> | |
| − | < | + | |
| − | <p> | + | |
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">7/29</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">pBAR_RFP checked with KOD polymerase PCR (contaminated NC)</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">8/1</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">RFP checked with KOD PCR</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">8/3</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | < | + | <li><p style="font-size: 18px;">RFP digested with EcoRI and BamHI for the ligation with C120_empty</p></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
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</div> | </div> | ||
Latest revision as of 17:07, 19 October 2016
RFP
RFP
7/9
BBa_E1010 T-streaked onto agar plates
7/10
BBa_E1010 extracted plasmid and checked with restriction digestion (EocRI and PstI 747bp and 2029bp)
7/16
BBa_E1010 checked with plasmid PCR (Primers: VF2-VR) (failed, no expected product)
7/17
BBa_E1010 checked again with plasmid PCR (failed, probably due to primer VF2-FR malfunction)
7/18
BBa_E1010 checked with plasmid PCR with newly design primers
7/19
BBa_E1010 checked with plasmid PCR with the addition of Mg2+
7/22
BBa_E1010 checked with Taq polymerase plasmid PCR (successful)
7/25
BBa_E1010 amplified with KOD polymerase PCR
7/26
7/29
pBAR_RFP checked with KOD polymerase PCR (contaminated NC)
8/1
RFP checked with KOD PCR
8/3
RFP digested with EcoRI and BamHI for the ligation with C120_empty
