Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-TtrpC"

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<div id="wrap">
 
<div id="wrap">
 +
 
<div class="imageimage1">
 
<div class="imageimage1">
 
<div class="wordword1">
 
<div class="wordword1">
  
−
<h1>TITLE</h1><hr />
+
<div class="satisfy">
−
<p>Besides the fungal killing switch and the functional prototype that help reduce concerns over GMO</p>
+
<h1>TtrpC</h1></div>
 +
<div class="satisfysp">
 +
<h1>TtrpC</h1></div>
 +
<hr />
  
−
</div></div></div>
+
</div></div><br /></div>
  
 
<div class="expand">
 
<div class="expand">
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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/22</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">Transformed plasmid pAN7-1 into competent cells</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">plasmid pAN7-1 containing cultures used in 2 in 1</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">Week 2: (1-7 July)</div>
+
<div class="technology">7/23</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>The ordered primers were taken this week.</p>
+
<li><p style="font-size: 18px;">pAN7-1 plasmid extraction</p></li>
−
<p>We would produce our biobricks, but the result was not like we expected after electrophoresis. Then we made colony PCR  and again do it for SOD and GPX. After this, we did digestion and also pTRE vector .</p>
+
−
<img src="https://static.igem.org/mediawiki/2014/7/7e/ATOMS-Notebook1.tif.png">
+
−
<img src="https://static.igem.org/mediawiki/2014/1/17/ATOMS-Notebook2.png">
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/24</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pAN7-1 amplified with plasmid PCR (primer: minPgpdA)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">pAN7-1 extracted and transformed cultures cryopreserved</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/29</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">7/31</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">VP-TR trouble shooting</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">VP-TR PCR amplification(correct)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<img src="" width="100%" />
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<p style="font-size: 18px;">Target sequence: TtrpC :791 base pairs</p>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/13</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC PCR amplification for the ligation with EL222_RFP</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/15</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC and pVP-EL222 PCR amplificaiton</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/16</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC digestion</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/23</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC amplified with KOD polymerase</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC digestion</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/26</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC checked with plasmid PCR (with remixed pVP-EL222 primers)</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">8/27</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC KOD PCR amplification</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">9/11</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC amplified with KOD polymerase</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">TtrpC amplified with KOD polymerase</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">transformation efficiency </div>
+
<div class="technology">9/19</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
<li><p style="font-size: 18px;">Amplified TtrpC form plasmid pAN7-1</p></li>
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
−
<p>To check our cell’s transformation efficiency 5 bacteria plates are transformed with different concentrated</p>
+
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 +
</div>
 +
 +
<div class="technology">10/1</div>
 +
<div class="thelanguage">
 +
<li><p style="font-size: 18px;">TtrpC digestion (for the ligation with pBARGPE1)</p></li>
 +
 +
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p>
 
</div>
 
</div>
  

Latest revision as of 17:22, 19 October 2016

TtrpC

TtrpC


[Collapse all] | [Expand all]

7/22

  • Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station

  • Transformed plasmid pAN7-1 into competent cells

  • plasmid pAN7-1 containing cultures used in 2 in 1

    • [Collapse]

    7/23

  • pAN7-1 plasmid extraction

    • [Collapse]

    7/24

  • pAN7-1 amplified with plasmid PCR (primer: minPgpdA)

  • pAN7-1 extracted and transformed cultures cryopreserved

    • [Collapse]

    7/29

  • VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)

    • [Collapse]

    7/31

  • VP-TR trouble shooting

  • VP-TR PCR amplification(correct)

    • Target sequence: TtrpC :791 base pairs

    [Collapse]

    8/13

  • TtrpC PCR amplification for the ligation with EL222_RFP

    • [Collapse]

    8/15

  • TtrpC and pVP-EL222 PCR amplificaiton

    • [Collapse]

    8/16

  • TtrpC digestion

    • [Collapse]

    8/23

  • TtrpC amplified with KOD polymerase

  • TtrpC digestion

    • [Collapse]

    8/26

  • TtrpC checked with plasmid PCR (with remixed pVP-EL222 primers)

    • [Collapse]

    8/27

  • TtrpC KOD PCR amplification

    • [Collapse]

    9/11

  • TtrpC amplified with KOD polymerase

  • TtrpC amplified with KOD polymerase

    • [Collapse]

    9/19

  • Amplified TtrpC form plasmid pAN7-1

    • [Collapse]

    10/1

  • TtrpC digestion (for the ligation with pBARGPE1)

    • [Collapse]