Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-TtrpC"
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<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station</p></li> | <li><p style="font-size: 18px;">Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station</p></li> | ||
| − | <li><p style="font-size: 18px;">pAN7-1 | + | <li><p style="font-size: 18px;">Transformed plasmid pAN7-1 into competent cells</p></li> |
| − | <li><p style="font-size: 18px;">pAN7-1 | + | <li><p style="font-size: 18px;">plasmid pAN7-1 containing cultures used in 2 in 1</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | ||
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<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">VP-TR trouble shooting</p></li> | <li><p style="font-size: 18px;">VP-TR trouble shooting</p></li> | ||
| − | <li><p style="font-size: 18px;">VP-TR PCR amplification</p></li> | + | <li><p style="font-size: 18px;">VP-TR PCR amplification(correct)</p></li> |
| + | <img src="" width="100%" /> | ||
| + | <p style="font-size: 18px;">Target sequence: TtrpC :791 base pairs</p> | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p> | ||
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<div class="technology">8/13</div> | <div class="technology">8/13</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">TtrpC PCR amplification for | + | <li><p style="font-size: 18px;">TtrpC PCR amplification for the ligation with EL222_RFP</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | ||
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<div class="technology">8/15</div> | <div class="technology">8/15</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">TtrpC and pVP- EL222 PCR amplificaiton</p></li> | + | <li><p style="font-size: 18px;">TtrpC and pVP-EL222 PCR amplificaiton</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p> | ||
| Line 181: | Line 183: | ||
<div class="technology">8/23</div> | <div class="technology">8/23</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">TtrpC | + | <li><p style="font-size: 18px;">TtrpC amplified with KOD polymerase</p></li> |
<li><p style="font-size: 18px;">TtrpC digestion</p></li> | <li><p style="font-size: 18px;">TtrpC digestion</p></li> | ||
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<div class="technology">8/26</div> | <div class="technology">8/26</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">TtrpC | + | <li><p style="font-size: 18px;">TtrpC checked with plasmid PCR (with remixed pVP-EL222 primers)</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p> | ||
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</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">9/11</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">TtrpC KOD | + | <li><p style="font-size: 18px;">TtrpC amplified with KOD polymerase</p></li> |
| − | <li><p style="font-size: 18px;">TtrpC KOD | + | <li><p style="font-size: 18px;">TtrpC amplified with KOD polymerase</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
| − | <div class="technology"> | + | <div class="technology">9/19</div> |
<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">Amplified TtrpC form plasmid pAN7-1</p></li> | <li><p style="font-size: 18px;">Amplified TtrpC form plasmid pAN7-1</p></li> | ||
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p> | ||
| + | </div> | ||
| + | |||
| + | <div class="technology">10/1</div> | ||
| + | <div class="thelanguage"> | ||
| + | <li><p style="font-size: 18px;">TtrpC digestion (for the ligation with pBARGPE1)</p></li> | ||
| + | |||
| + | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p> | ||
</div> | </div> | ||
Latest revision as of 17:22, 19 October 2016
TtrpC
TtrpC
7/22
Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station
Transformed plasmid pAN7-1 into competent cells
plasmid pAN7-1 containing cultures used in 2 in 1
7/23
pAN7-1 plasmid extraction
7/24
pAN7-1 amplified with plasmid PCR (primer: minPgpdA)
pAN7-1 extracted and transformed cultures cryopreserved
7/29
VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)
7/31
VP-TR trouble shooting
VP-TR PCR amplification(correct)
Target sequence: TtrpC :791 base pairs
8/13
TtrpC PCR amplification for the ligation with EL222_RFP
8/15
TtrpC and pVP-EL222 PCR amplificaiton
8/16
TtrpC digestion
8/23
8/26
TtrpC checked with plasmid PCR (with remixed pVP-EL222 primers)
8/27
TtrpC KOD PCR amplification
9/11
9/19
Amplified TtrpC form plasmid pAN7-1
10/1
TtrpC digestion (for the ligation with pBARGPE1)
