Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-TtrpC"
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<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station</p></li> | <li><p style="font-size: 18px;">Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station</p></li> | ||
| − | <li><p style="font-size: 18px;">pAN7-1 | + | <li><p style="font-size: 18px;">Transformed plasmid pAN7-1 into competent cells</p></li> |
| − | <li><p style="font-size: 18px;">pAN7-1 | + | <li><p style="font-size: 18px;">plasmid pAN7-1 containing cultures used in 2 in 1</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p> | ||
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<div class="technology">8/13</div> | <div class="technology">8/13</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">TtrpC PCR amplification for EL222_RFP</p></li> | + | <li><p style="font-size: 18px;">TtrpC PCR amplification for the ligation with EL222_RFP</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p> | ||
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<div class="technology">8/23</div> | <div class="technology">8/23</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">TtrpC | + | <li><p style="font-size: 18px;">TtrpC amplified with KOD polymerase</p></li> |
<li><p style="font-size: 18px;">TtrpC digestion</p></li> | <li><p style="font-size: 18px;">TtrpC digestion</p></li> | ||
Latest revision as of 17:22, 19 October 2016
TtrpC
TtrpC
Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station
Transformed plasmid pAN7-1 into competent cells
plasmid pAN7-1 containing cultures used in 2 in 1
pAN7-1 plasmid extraction
pAN7-1 amplified with plasmid PCR (primer: minPgpdA)
pAN7-1 extracted and transformed cultures cryopreserved
VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)
VP-TR trouble shooting
VP-TR PCR amplification(correct)
Target sequence: TtrpC :791 base pairs
TtrpC PCR amplification for the ligation with EL222_RFP
TtrpC and pVP-EL222 PCR amplificaiton
TtrpC digestion
TtrpC checked with plasmid PCR (with remixed pVP-EL222 primers)
TtrpC KOD PCR amplification
Amplified TtrpC form plasmid pAN7-1
TtrpC digestion (for the ligation with pBARGPE1)
