Difference between revisions of "Team:Paris Bettencourt/Safety"

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{{Paris_Bettencourt}}
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{{Paris_Bettencourt/Menu}}
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{{Paris_Bettencourt/Wiki}}
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<br>
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<!--h1 class="red">Lab safety </h1-->
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<div id=subheader>
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<div id="input">
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<h2 class="red">Summary</h2>
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<p>
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In iGEM we take safety seriously. Our project touches sensible topics such as enzyme and human contact so we had a special consideration about safety issues. From strict lab rules to interviews with drycleaners, we tried to measure user's awareness and potential problems in the implementation and safety of our final engineered GMO (genetically modified microorganism).
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</p>
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<h2 class="red">Safety Training and Lab Rules</h2>
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<div id="figurebox">
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<img src="https://static.igem.org/mediawiki/2016/f/f0/Paris_Bettencourt-File_vue_cochin.jpg" alt="Quercetin strains degradation" style="width:900px;">
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<p>
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<b> Figure 1: Our laboratory facilities at the Cochin institut in Paris.</b>
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</div>
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<p>
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At the beginning of the competition, all team members received a safety training for working in the Biosafety Level 1 and in the Biosafety level 2 laboratories. This training was conducted by the Biosafety officer for INSERM U1001 (Fig. 1) and it included best laboratory practices as described in the WHO laboratory biosafety manual.<br>
 +
It included rules about protocols in the lab, protective clothing and equipment, decontamination methods and practices, prevention of the transfer of genetic material and microorganisms, handling chemicals, use of machines and emergency procedures. The safety form was submitted to iGEM HQ as well.
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</p>
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<h2 class="red">Biosafety Level 2</h2>
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<p>
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During this project we aimed, amongst other things, to create a database of strains isolated from vineyard soils from all around the world. Since we were handeling samples without knowing the microbes that were present on them, we took special security measurements. To begin with, we worked in a lab with biosafety level 2, in the appropriated hood and wearing the appropriate safety clothing. We also chose to work with a set of media that would be selective for non-pathogenical species, not allowing the potentially dangerous species that were on the samples to grow.
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</p>
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<h2 class="red">Organisms used</h2>
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<p>
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Our sole chassis organism in this project was <i>E. coli</i>. All cloning steps were performed in the DH5alpha strain, and all protein expression steps on the BL21(DE3) strain.<br>
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In the making of our database we isolated several different species of Acidovorax, Actinomycetes, Arthrobacter, Bacillus, Brevibacterium, Celullosimicrobium, Corynebacterium, Escherichia, Flavobacterium, Klebsiella, Kocuria, Microbacterium, Micrococcus, Oerskavia, Paenibacillus, Pantoea, Pseudomonas, Rhodococcus, Staphylococcus (non aureus) and Streptomyces. All our samples were sent for sequencing after growing them in selective media and no harmful species for human health was isolated from them.
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</p>
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<h2 class="red">References</h2>
 
<ul>
 
<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Public Health Statement for Tetrachloroethylene (PERC) from the Agency for Toxic Substances & Disease Registry of the United States Department of Health and Human Services (2014)
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
 
</ul>
  
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</div>
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</div> <! End input Div> 
  
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<div class="column half_size">
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</div> <! End subheader Div>  
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
  
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</div>
 
  
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<div class="column half_size">
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<div class=separation></div>
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<h5>Safe Shipment</h5>
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<div style="clear: both;"></div>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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</div>
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</div>
 
</div>
 
</div>
  
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</body>
  
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{{Paris_Bettencourt/Footer}}

Latest revision as of 17:42, 19 October 2016




Summary

In iGEM we take safety seriously. Our project touches sensible topics such as enzyme and human contact so we had a special consideration about safety issues. From strict lab rules to interviews with drycleaners, we tried to measure user's awareness and potential problems in the implementation and safety of our final engineered GMO (genetically modified microorganism).

Safety Training and Lab Rules

Quercetin strains degradation

Figure 1: Our laboratory facilities at the Cochin institut in Paris.

At the beginning of the competition, all team members received a safety training for working in the Biosafety Level 1 and in the Biosafety level 2 laboratories. This training was conducted by the Biosafety officer for INSERM U1001 (Fig. 1) and it included best laboratory practices as described in the WHO laboratory biosafety manual.
It included rules about protocols in the lab, protective clothing and equipment, decontamination methods and practices, prevention of the transfer of genetic material and microorganisms, handling chemicals, use of machines and emergency procedures. The safety form was submitted to iGEM HQ as well.

Biosafety Level 2

During this project we aimed, amongst other things, to create a database of strains isolated from vineyard soils from all around the world. Since we were handeling samples without knowing the microbes that were present on them, we took special security measurements. To begin with, we worked in a lab with biosafety level 2, in the appropriated hood and wearing the appropriate safety clothing. We also chose to work with a set of media that would be selective for non-pathogenical species, not allowing the potentially dangerous species that were on the samples to grow.

Organisms used

Our sole chassis organism in this project was E. coli. All cloning steps were performed in the DH5alpha strain, and all protein expression steps on the BL21(DE3) strain.
In the making of our database we isolated several different species of Acidovorax, Actinomycetes, Arthrobacter, Bacillus, Brevibacterium, Celullosimicrobium, Corynebacterium, Escherichia, Flavobacterium, Klebsiella, Kocuria, Microbacterium, Micrococcus, Oerskavia, Paenibacillus, Pantoea, Pseudomonas, Rhodococcus, Staphylococcus (non aureus) and Streptomyces. All our samples were sent for sequencing after growing them in selective media and no harmful species for human health was isolated from them.

References

  • Public Health Statement for Tetrachloroethylene (PERC) from the Agency for Toxic Substances & Disease Registry of the United States Department of Health and Human Services (2014)
Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
igem2016parisbettencourt@gmail.com
2016.igem.org