Difference between revisions of "Team:NUS Singapore/Notebook/Group3"
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| − | + | <title>Interactive Points | Codrops</title> | |
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| − | + | <span><img src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png"></span> | |
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| − | + | <div id="header1" class="ha-header-front" style="height: 120px"> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore"><img id="nus_logo" src="https://static.igem.org/mediawiki/2016/1/1d/T--NUS_Singapore--header_left.png" style="height: 130%; margin-top: -10px; left: 0px; position: absolute;"/></a> | |
| − | + | <a href="http://www.science.nus.edu.sg"><img id="sci_logo" src="https://static.igem.org/mediawiki/2016/2/21/T--NUS_Singapore--NUS_Science_logo_grey.png" style="height: 70%; margin-top: 25px; right: 0px; position: absolute;"/></a> | |
| − | + | <a href="http://sps.nus.edu.sg/alpha"><img id="sps_logo" src="https://static.igem.org/mediawiki/2016/e/ec/T--NUS_Singapore--SPS_Logo_High_Res_grey.png" style="height: 60%; margin-top: 25px; right: 280px; position: absolute;"/></a> | |
| − | + | <div style="height: 7%; background-color: rgb(217, 215, 215); width: 100%; bottom: 0px; left: 0px; position: absolute;"></div> | |
| − | + | </div> | |
| − | + | <div class="ha-header-bottom" style="text-align: center;"> | |
| − | + | <a><h1><a href="https://2016.igem.org/Team:NUS_Singapore" style="color: black; font-weight: lighter;">iGEM 2016 NUS SINGAPORE</a></h1></a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </header> | |
| − | + | <!--End of Header--> | |
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| − | + | <nav class="st-menu st-effect-1 list-group panel" id="menu-1" style="overflow: auto; z-index: 100; background-color: #E59500;"> | |
| − | <span><img src="https://static.igem.org/mediawiki/2016/1/1e/T--NUS_Singapore--practice.png" style="width: 30px; height: auto; top: 35px; left: 35px; position: absolute;"><h4 style=" left: 75px; top: 25px; position: absolute; font-size: 17px;">NUS_SINGAPORE</h4></span> | + | <span><img src="https://static.igem.org/mediawiki/2016/1/1e/T--NUS_Singapore--practice.png" style="width: 30px; height: auto; top: 35px; left: 35px; position: absolute;"><h4 style=" left: 75px; top: 25px; position: absolute; font-size: 17px; color: white;">NUS_SINGAPORE</h4></span> |
| − | + | <ul> | |
| − | + | <li><a class="icon icon-home" id="menu-item-1" href="https://2016.igem.org/Team:NUS_Singapore">Home</a></li> | |
| − | + | <li><a class="icon icon-project" onclick="growDiv('2')" id="menu-item-2" href="https://2016.igem.org/Team:NUS_Singapore/Project">Project | |
| − | + | <span><img id="sidebar-arrow-2" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | |
| − | + | <div class="grow" id="grow-2"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Project/Wet_Lab" class="list-group-item" style="color: rgb(179, 179, 179);">Overview</a> | |
| − | + | ||
<a href="https://2016.igem.org/Team:NUS_Singapore/Model" class="list-group-item" style="color: rgb(179, 179, 179);">Modelling</a> | <a href="https://2016.igem.org/Team:NUS_Singapore/Model" class="list-group-item" style="color: rgb(179, 179, 179);">Modelling</a> | ||
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | <li><a class="icon icon-notebook" id="menu-item-9" onclick="growDiv('9')" href="https://2016.igem.org/Team:NUS_Singapore/Notebook"> | + | <li><a class="icon icon-notebook" id="menu-item-9" onclick="growDiv('9')" href="https://2016.igem.org/Team:NUS_Singapore/Notebook">RIOT Sensor |
| − | + | <span><img id="sidebar-arrow-9" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | |
| − | + | <div class="grow" id="grow-9"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Description" class="list-group-item" style="color: rgb(179, 179, 179);">Description</a> | |
<a href="https://2016.igem.org/Team:NUS_Singapore/Proof" class="list-group-item" style="color: rgb(179, 179, 179);">Proof of Concept</a> | <a href="https://2016.igem.org/Team:NUS_Singapore/Proof" class="list-group-item" style="color: rgb(179, 179, 179);">Proof of Concept</a> | ||
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Demonstrate" class="list-group-item" style="color: rgb(179, 179, 179);">Demonstration</a> | |
| − | + | ||
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTResponder"> | + | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTResponder">RIOT Responder</a></li> |
| − | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTInvader"> | + | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTInvader">RIOT Invader</a></li> |
| − | + | <li><a class="icon icon-practice" onclick="growDiv('3')" id="menu-item-3" href="#">Human Practices | |
| − | + | <span><img id="sidebar-arrow-3" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"><span></a> | |
| − | + | <div class="grow" id="grow-3"> | |
| − | + | <div class='measuringWrapper'> | |
<a href="https://2016.igem.org/Team:NUS_Singapore/HP/Silver" class="list-group-item" style="color: rgb(179, 179, 179);">Survey</a> | <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Silver" class="list-group-item" style="color: rgb(179, 179, 179);">Survey</a> | ||
<a href="https://2016.igem.org/Team:NUS_Singapore/HP/Gold" class="list-group-item" style="color: rgb(179, 179, 179);">Business Plan</a> | <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Gold" class="list-group-item" style="color: rgb(179, 179, 179);">Business Plan</a> | ||
<a href="https://2016.igem.org/Team:NUS_Singapore/Integrated_Practices" class="list-group-item" style="color: rgb(179, 179, 179);">Integration</a> | <a href="https://2016.igem.org/Team:NUS_Singapore/Integrated_Practices" class="list-group-item" style="color: rgb(179, 179, 179);">Integration</a> | ||
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li><a class="icon icon-collaboration" onclick="growDiv('4')" id="menu-item-4" href="#">Collaborations | |
<span><img id="sidebar-arrow-4" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | <span><img id="sidebar-arrow-4" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | ||
| − | + | <div class="grow" id="grow-4"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#MELBOURNE" class="list-group-item" style="color: rgb(179, 179, 179);">MELBOURNE</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKUST" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKUST</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKU" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKU</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
<li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Parts">Parts Registry</a></li> | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Parts">Parts Registry</a></li> | ||
| − | + | <li><a class="icon icon-safety" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Safety">Safety</a></li> | |
| − | + | <li><a class="icon icon-team" id="menu-item-6" href="https://2016.igem.org/Team:NUS_Singapore/Team">Team</a></li> | |
| − | + | <li><a class="icon icon-notebook" id="menu-item-7" onclick="growDiv('7')" href="https://2016.igem.org/Team:NUS_Singapore/Notebook">Notebook | |
| − | + | <span><img id="sidebar-arrow-7" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | |
| − | + | <div class="grow" id="grow-7"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group1" class="list-group-item" style="color: rgb(179, 179, 179);">I. RIOT Sensor</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group2" class="list-group-item" style="color: rgb(179, 179, 179);">II. RIOT Responder</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group3" class="list-group-item" style="color: rgb(179, 179, 179);">III. RIOT Invader</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li><a class="icon icon-acknowledgement" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Attributions">Attributions</a></li> | |
<li><a class="icon icon-cup" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Medals">Medals</a></li> | <li><a class="icon icon-cup" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Medals">Medals</a></li> | ||
| − | + | </ul> | |
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| − | + | <div class="demos"> | |
| − | + | <a class="current-demo" href="index.html">Default Demo</a> | |
| − | + | <a href="index2.html">Perspective Demo (Webkit only)</a> | |
| − | </div> | + | </div> |
| − | <div style="position: absolute; height: 250px; width: 100%; background-color: black; z-index: -1;"></div> | + | <div style="position: absolute; height: 250px; width: 100%; background-color: black; z-index: -1;"></div> |
| − | <h2 class="ss-subtitle">Wet Lab Notebook: RIOT RESPONDER</h2> | + | <h2 class="ss-subtitle">Wet Lab Notebook: RIOT RESPONDER</h2> |
| − | <div id="ss-links" class="ss-links" style="z-index: 99;"> | + | <div id="ss-links" class="ss-links" style="z-index: 99;"> |
| − | + | <a href="#st-container">Jun</a> | |
| − | + | <a href="#july">Jul</a> | |
| − | + | <a href="#august">Aug</a> | |
| − | + | <a href="#september">Sep</a> | |
| − | + | <a href="#october">Oct</a> | |
| − | </div> | + | </div> |
| − | <div id="ss-container" class="ss-container" style="background-color: white;"> | + | <div id="ss-container" class="ss-container" style="background-color: white;"> |
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| − | + | <div class="ss-left" style="left: 2%;"> | |
| − | + | <h2 id="may" class="">Jun</h2> | |
| − | + | </div> | |
| − | + | <div class="ss-right" style="right: -2%;"> | |
| − | + | <h2>2016</h2> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="ss-row ss-large"> | |
| − | + | <div class="ss-left"> | |
| − | + | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | |
| + | <p>Overall Strategies</p> | ||
| − | + | <p><img src="https://static.igem.org/mediawiki/2016/1/13/T--NUS_Singapore--log3_1.png" style="width: 100%;"></p> | |
| − | + | <p><img src="https://static.igem.org/mediawiki/2016/2/25/T--NUS_Singapore--log3_0.png" style="width: 100%;"></p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="ss-right"> | |
| − | + | <h3> | |
| − | + | <span>1 June, 2016</span> | |
| − | + | <a href="http://tympanus.net/Tutorials/TypographyEffects/">Milestone 1 : Overall cloning design</a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | + | </div> | |
| − | <div class="ss-row ss-large"> | + | <div class="ss-row ss-large"> |
| − | + | <div class="ss-left"> | |
| − | + | <h3> | |
| − | + | <span>12 June, 2016</span> | |
| − | + | <a>Milestone 2: Construction of pConst-LuxR-HA</a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | + | <div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> | |
| − | + | <div class="log-content ss-circle-deco"> | |
| − | + | <span><p>Construct 1: </p><p><img src="https://static.igem.org/mediawiki/2016/9/9f/T--NUS_Singapore--log3_8.png" style="width: 100%;"></p></span> | |
| − | + | <p><img src="https://static.igem.org/mediawiki/2016/e/e7/T--NUS_Singapore--log3_2.png" style="width: 100%;"></p> | |
| − | + | <p> | |
| − | + | <span style="font-size: larger;">Fig 1. A.</span> LuxR gene amplified from iGEM distribution 2016 with designed primers GEM002 and GEM005. The expected band size of LuxR with overhang is 795 bp. <span style="font-size: larger;">B.</span>LuxR stitiched with terminater (BBa_B0015) with overlapping PCR. <span style="font-size: larger;">C.</span> Constructed full-length RBS-pConstitutive-LuxR-HA-Terminator with expected size of 1034 bp. | |
| − | + | </p> | |
| − | + | ||
| − | + | </div> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | + | <div class="ss-row"> | |
| − | + | <div class="ss-left"> | |
| − | + | <h2 id="july" class="offset">July</h2> | |
| − | + | </div> | |
| − | + | <div class="ss-right offset"> | |
| − | + | <h2>2016</h2> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="ss-row ss-large"> | |
| − | + | <div class="ss-left"> | |
| − | + | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | |
| − | + | <span><p>Construct 2: </p><p><img src="https://static.igem.org/mediawiki/2016/6/61/T--NUS_Singapore--log3_9.png" style="width: 100%;"></p></span> | |
| − | + | <p><img src="https://static.igem.org/mediawiki/2016/2/22/T--NUS_Singapore--log3_3.png" style="width: 100%;"></p> | |
| − | + | <p> | |
| − | + | <span style="font-size: larger;">Fig 2. A.</span> LuxI gene is cloned from iGEM2016 distribution kit with primers GEM008 and GEM011. The observed band size of LuxI with overhangs is close to the expected band size of 629 bp. <span style="font-size: larger;">B.</span>Terminator BBa_B0015 is amplified using GEM009 and 012 and stitiched with LuxI. <span style="font-size: larger;">C.</span> Promoter-RBS fragment is synthesized and stitiched to LuxI-terminator by PCR reaction. The observed band size for full LuxI construct is close to the expected band size of 936 bp. | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="ss-right"> | |
| − | + | <h3> | |
| − | + | <span>4 July, 2016</span> | |
| − | + | <a>Milestone 3: : Construction of pLuxR-LuxI-HA </a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | <div class="ss-row ss-large"> | + | <div class="ss-row ss-large"> |
| − | + | <div class="ss-left"> | |
| − | + | <h3> | |
| − | + | <span>11 July, 2016</span> | |
| − | + | <a>Milestone 4: Screening of pLuxR-invasin constructs </a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | + | <div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> | |
| − | + | <div class="log-content ss-circle-deco"> | |
| − | <p><img src="https://static.igem.org/mediawiki/2016/c/c6/T--NUS_Singapore--log3_16.png" style="width: 100%;"></p> | + | <p><img src="https://static.igem.org/mediawiki/2016/c/c6/T--NUS_Singapore--log3_16.png" style="width: 100%;"></p> |
| − | + | </div> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | <div class="ss-row"> | + | <div class="ss-row"> |
| − | + | <div class="ss-left"> | |
| − | + | <h2 id="august" class="offset">Aug</h2> | |
| − | + | </div> | |
| − | + | <div class="ss-right offset"> | |
| − | + | <h2>2016</h2> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | <div class="ss-row ss-large"> | + | <div class="ss-row ss-large"> |
| − | + | <div class="ss-left"> | |
| − | + | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | |
| − | <p><img src="https://static.igem.org/mediawiki/2016/0/08/T--NUS_Singapore--log3_17.png" style="width: 100%;"></p> | + | <p><img src="https://static.igem.org/mediawiki/2016/0/08/T--NUS_Singapore--log3_17.png" style="width: 100%;"></p> |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="ss-right"> | |
| − | + | <h3> | |
| − | + | <span>1 Aug, 2016</span> | |
| − | + | <a>Milestone 5: Screening of pLuxR-LLO construct</a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | <div class="ss-row ss-large"> | + | <div class="ss-row ss-large"> |
| − | + | <div class="ss-left"> | |
| − | + | <h3> | |
| − | + | <span>8 Aug, 2016</span> | |
| − | + | <a>Milestone 6: Construction of pLuxR-GFP plasmid</a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | + | <div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> | |
| − | + | <div class="log-content ss-circle-deco"> | |
| − | <p><img src="https://static.igem.org/mediawiki/2016/2/2b/T--NUS_Singapore--log3_18.png" style="width: 100%;"></p> | + | <p><img src="https://static.igem.org/mediawiki/2016/2/2b/T--NUS_Singapore--log3_18.png" style="width: 100%;"></p> |
| − | + | </div> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | <div class="ss-row ss-large"> | + | <div class="ss-row ss-large"> |
| − | + | <div class="ss-left"> | |
| − | + | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | |
| − | <p><img src="https://static.igem.org/mediawiki/2016/a/a3/T--NUS_Singapore--log3_19.png" style="width: 100%;"></p> | + | <p><img src="https://static.igem.org/mediawiki/2016/a/a3/T--NUS_Singapore--log3_19.png" style="width: 100%;"></p> |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="ss-right"> | |
| − | + | <h3> | |
| − | + | <span>1 Aug, 2016</span> | |
| − | + | <a>Milestone 7: Testing the assembled pConst-LuxR-pLuxR-LuxI construct</a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | <div class="ss-row"> | + | <div class="ss-row"> |
| − | + | <div class="ss-left"> | |
| − | + | <h2 id="september" class="offset">Sep</h2> | |
| − | + | </div> | |
| − | + | <div class="ss-right offset"> | |
| − | + | <h2>2016</h2> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | <div class="ss-row ss-large"> | + | <div class="ss-row ss-large"> |
| − | + | <div class="ss-left"> | |
| − | + | <h3> | |
| − | + | <span>19 Sep, 2016</span> | |
| − | + | <a>Milestone 8: Testing the assembled pConst-LuxR-pLuxR-LLO construct</a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | + | <div class="ss-right ha-waypoint" data-animate-down="ha-header-rotate" data-animate-up="ha-header-rotateBack"> | |
| − | + | <div class="log-content ss-circle-deco"> | |
| − | <p><img src="https://static.igem.org/mediawiki/2016/b/bf/T--NUS_Singapore--log3_20.png" style="width: 100%;"></p> | + | <p><img src="https://static.igem.org/mediawiki/2016/b/bf/T--NUS_Singapore--log3_20.png" style="width: 100%;"></p> |
| − | + | </div> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| − | + | <div class="ss-row"> | |
| − | + | <div class="ss-left"> | |
| − | + | <h2 id="october" class="offset">Oct</h2> | |
| − | + | </div> | |
| − | + | <div class="ss-right offset"> | |
| − | + | <h2>2016</h2> | |
| − | + | </div> | |
| − | + | </div> | |
| − | <div class="ss-row ss-large"> | + | <div class="ss-row ss-large"> |
| − | + | <div class="ss-left"> | |
| − | + | <div class="log-content ss-circle-deco" style="margin-top: 20px;"> | |
| − | <p><img src="https://static.igem.org/mediawiki/2016/6/64/T--NUS_Singapore--log3_211.png" style="width: 100%;"></p> | + | <p><img src="https://static.igem.org/mediawiki/2016/6/64/T--NUS_Singapore--log3_211.png" style="width: 100%;"></p> |
| − | + | </div> | |
| − | + | </div> | |
| − | + | <div class="ss-right"> | |
| − | + | <h3> | |
| − | + | <span>10 Oct, 2016</span> | |
| − | + | <a>Milestone 9: Testing the assembled pConst-LuxR-pLuxR-GFP construct</a> | |
| − | + | </h3> | |
| − | + | </div> | |
| − | </div> | + | </div> |
| + | </div> | ||
| + | </div> | ||
</div> | </div> | ||
| − | |||
| − | |||
| Line 329: | Line 330: | ||
| − | <section class="content content--related"> | + | <section class="content content--related"> |
| − | + | <ul class="copyright"> | |
<li>© NUS_Singapore iGEM 2016</li> | <li>© NUS_Singapore iGEM 2016</li> | ||
| − | + | </ul> | |
| − | </section> | + | </section> |
| − | <a href="#" class="go-top">Go To Top</a> | + | <a href="#" class="go-top">Go To Top</a> |
| − | </div><!-- /st-pusher --> | + | </div><!-- /st-pusher --> |
</div><!-- /st-container --> | </div><!-- /st-container --> | ||
</main> | </main> | ||
<script> | <script> | ||
| − | + | $(function() { | |
| − | + | var $sidescroll = (function() { | |
// the row elements | // the row elements | ||
| Line 376: | Line 377: | ||
// if perspective add css | // if perspective add css | ||
if( perspective ) { | if( perspective ) { | ||
| − | + | $rows.css({ | |
| − | + | '-webkit-perspective' : 600, | |
| − | + | '-webkit-perspective-origin' : '50% 0%' | |
| − | + | }); | |
} | } | ||
// show the pointers for the inviewport rows | // show the pointers for the inviewport rows | ||
| Line 386: | Line 387: | ||
placeRows(); | placeRows(); | ||
| − | + | }, | |
// defines a selector that gathers the row elems that are initially visible. | // defines a selector that gathers the row elems that are initially visible. | ||
// the element is visible if its top is less than the window's height. | // the element is visible if its top is less than the window's height. | ||
| Line 392: | Line 393: | ||
defineViewport = function() { | defineViewport = function() { | ||
| − | + | $.extend( $.expr[':'], { | |
| − | + | inviewport : function ( el ) { | |
| − | + | if ( $(el).offset().top < winSize.height ) { | |
| − | + | return true; | |
| − | + | } | |
| − | + | return false; | |
| − | + | } | |
| − | + | }); | |
}, | }, | ||
| Line 407: | Line 408: | ||
setViewportRows = function() { | setViewportRows = function() { | ||
| − | + | $rowsViewport = $rows.filter(':inviewport'); | |
| − | + | $rowsOutViewport = $rows.not( $rowsViewport ) | |
| − | + | ||
}, | }, | ||
// get window sizes | // get window sizes | ||
getWinSize = function() { | getWinSize = function() { | ||
| − | + | winSize.width = $win.width(); | |
| − | + | winSize.height = $win.height(); | |
}, | }, | ||
| Line 427: | Line 428: | ||
// scroll to the element that has id = menu's href | // scroll to the element that has id = menu's href | ||
$('html, body').stop().animate({ | $('html, body').stop().animate({ | ||
| − | + | scrollTop: $( $(this).attr('href') ).offset().top | |
}, scollPageSpeed, scollPageEasing ); | }, scollPageSpeed, scollPageEasing ); | ||
return false; | return false; | ||
| − | + | }); | |
$(window).on({ | $(window).on({ | ||
| Line 447: | Line 448: | ||
$rowsViewport.each( function() { | $rowsViewport.each( function() { | ||
| − | + | $(this).find('div.ss-left') | |
| − | + | .css({ left : '0%' }) | |
| − | + | .end() | |
| − | + | .find('div.ss-right') | |
| − | + | .css({ right : '0%' }) | |
| − | + | .end() | |
| − | + | .find('a.ss-circle') | |
| − | + | .addClass('ss-circle-deco'); | |
}); | }); | ||
| − | + | }, | |
// when scrolling the page change the position of each row | // when scrolling the page change the position of each row | ||
'scroll.Scrolling' : function( event ) { | 'scroll.Scrolling' : function( event ) { | ||
| Line 468: | Line 469: | ||
setTimeout( function() { | setTimeout( function() { | ||
| − | + | placeRows(); | |
| − | + | anim = false; | |
| − | + | ||
}, 10 ); | }, 10 ); | ||
| − | + | } | |
| − | + | }); | |
| − | + | }, | |
// sets the position of the rows (left and right row elements). | // sets the position of the rows (left and right row elements). | ||
// Both of these elements will start with -50% for the left/right (not visible) | // Both of these elements will start with -50% for the left/right (not visible) | ||
| Line 491: | Line 492: | ||
$rowsOutViewport.each( function(i) { | $rowsOutViewport.each( function(i) { | ||
| − | + | var $row = $(this), | |
// the left side element | // the left side element | ||
$rowL = $row.find('div.ss-left'), | $rowL = $row.find('div.ss-left'), | ||
| Line 502: | Line 503: | ||
if( rowT > winSize.height + winscroll ) { | if( rowT > winSize.height + winscroll ) { | ||
| − | + | if( perspective ) { | |
| − | + | $rowL.css({ | |
| − | + | '-webkit-transform' : 'translate3d(-75%, 0, 0) rotateY(-90deg) translate3d(-75%, 0, 0)', | |
| − | + | 'opacity' : 0 | |
| − | + | }); | |
| − | + | $rowR.css({ | |
| − | + | '-webkit-transform' : 'translate3d(75%, 0, 0) rotateY(90deg) translate3d(75%, 0, 0)', | |
| − | + | 'opacity' : 0 | |
| − | + | }); | |
| − | + | } | |
| − | + | else { | |
| − | + | $rowL.css({ left : '-50%' }); | |
| − | + | $rowR.css({ right : '-50%' }); | |
| − | + | } | |
| − | + | ||
} | } | ||
// if not, the row should become visible (0% of left/right) as it gets closer to the center of the screen. | // if not, the row should become visible (0% of left/right) as it gets closer to the center of the screen. | ||
| Line 538: | Line 539: | ||
if( !$row.data('pointer') ) { | if( !$row.data('pointer') ) { | ||
| − | + | $row.data( 'pointer', true ); | |
| − | + | $row.find('.ss-circle').addClass('ss-circle-deco'); | |
} | } | ||
| − | + | } | |
| − | + | else { | |
// the pointer should not be shown | // the pointer should not be shown | ||
if( $row.data('pointer') ) { | if( $row.data('pointer') ) { | ||
| − | + | $row.data( 'pointer', false ); | |
| − | + | $row.find('.ss-circle').removeClass('ss-circle-deco'); | |
} | } | ||
| − | + | } | |
| − | + | ||
// set calculated values | // set calculated values | ||
if( perspective ) { | if( perspective ) { | ||
| − | + | var t = Math.max( factor * 75, 0 ), | |
| − | + | r = Math.max( factor * 90, 0 ), | |
| − | + | o = Math.min( Math.abs( factor - 1 ), 1 ); | |
| − | + | ||
| − | + | $rowL.css({ | |
| − | + | '-webkit-transform' : 'translate3d(-' + t + '%, 0, 0) rotateY(-' + r + 'deg) translate3d(-' + t + '%, 0, 0)', | |
| − | + | 'opacity' : o | |
| − | + | }); | |
| − | + | $rowR.css({ | |
| − | + | '-webkit-transform' : 'translate3d(' + t + '%, 0, 0) rotateY(' + r + 'deg) translate3d(' + t + '%, 0, 0)', | |
| − | + | 'opacity' : o | |
| − | + | }); | |
} | } | ||
else { | else { | ||
| − | + | $rowL.css({ left : - val + '%' }); | |
| − | + | $rowR.css({ right : - val + '%' }); | |
| − | + | ||
} | } | ||
| − | + | } | |
| − | + | }); | |
}; | }; | ||
Latest revision as of 17:44, 19 October 2016
<!DOCTYPE html>
Wet Lab Notebook: RIOT RESPONDER
Jun
2016
Overall Strategies
1 June, 2016 Milestone 1 : Overall cloning design
12 June, 2016 Milestone 2: Construction of pConst-LuxR-HA
Construct 1:
Fig 1. A. LuxR gene amplified from iGEM distribution 2016 with designed primers GEM002 and GEM005. The expected band size of LuxR with overhang is 795 bp. B.LuxR stitiched with terminater (BBa_B0015) with overlapping PCR. C. Constructed full-length RBS-pConstitutive-LuxR-HA-Terminator with expected size of 1034 bp.
July
2016
Construct 2:
Fig 2. A. LuxI gene is cloned from iGEM2016 distribution kit with primers GEM008 and GEM011. The observed band size of LuxI with overhangs is close to the expected band size of 629 bp. B.Terminator BBa_B0015 is amplified using GEM009 and 012 and stitiched with LuxI. C. Promoter-RBS fragment is synthesized and stitiched to LuxI-terminator by PCR reaction. The observed band size for full LuxI construct is close to the expected band size of 936 bp.
4 July, 2016 Milestone 3: : Construction of pLuxR-LuxI-HA
11 July, 2016 Milestone 4: Screening of pLuxR-invasin constructs
Aug
2016
1 Aug, 2016 Milestone 5: Screening of pLuxR-LLO construct
8 Aug, 2016 Milestone 6: Construction of pLuxR-GFP plasmid
