Difference between revisions of "Team:NUS Singapore/Notebook/Group3"
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| − | + | <nav class="st-menu st-effect-1 list-group panel" id="menu-1" style="overflow: auto; z-index: 100; background-color: #E59500;"> | |
| − | + | <span><img src="https://static.igem.org/mediawiki/2016/1/1e/T--NUS_Singapore--practice.png" style="width: 30px; height: auto; top: 35px; left: 35px; position: absolute;"><h4 style=" left: 75px; top: 25px; position: absolute; font-size: 17px; color: white;">NUS_SINGAPORE</h4></span> | |
| − | + | <ul> | |
| − | + | <li><a class="icon icon-home" id="menu-item-1" href="https://2016.igem.org/Team:NUS_Singapore">Home</a></li> | |
| − | + | <li><a class="icon icon-project" onclick="growDiv('2')" id="menu-item-2" href="https://2016.igem.org/Team:NUS_Singapore/Project">Project | |
| − | + | <span><img id="sidebar-arrow-2" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | |
| − | + | <div class="grow" id="grow-2"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Project/Wet_Lab" class="list-group-item" style="color: rgb(179, 179, 179);">Overview</a> | |
| − | + | ||
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Model" class="list-group-item" style="color: rgb(179, 179, 179);">Modelling</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li><a class="icon icon-notebook" id="menu-item-9" onclick="growDiv('9')" href="https://2016.igem.org/Team:NUS_Singapore/Notebook">RIOT Sensor | |
| − | + | <span><img id="sidebar-arrow-9" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | |
| − | + | <div class="grow" id="grow-9"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Description" class="list-group-item" style="color: rgb(179, 179, 179);">Description</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Proof" class="list-group-item" style="color: rgb(179, 179, 179);">Proof of Concept</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Demonstrate" class="list-group-item" style="color: rgb(179, 179, 179);">Demonstration</a> | |
| − | + | ||
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTResponder">RIOT Responder</a></li> | |
| − | + | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/RIOTInvader">RIOT Invader</a></li> | |
| − | + | <li><a class="icon icon-practice" onclick="growDiv('3')" id="menu-item-3" href="#">Human Practices | |
| − | + | <span><img id="sidebar-arrow-3" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"><span></a> | |
| − | + | <div class="grow" id="grow-3"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Silver" class="list-group-item" style="color: rgb(179, 179, 179);">Survey</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/HP/Gold" class="list-group-item" style="color: rgb(179, 179, 179);">Business Plan</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Integrated_Practices" class="list-group-item" style="color: rgb(179, 179, 179);">Integration</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li><a class="icon icon-collaboration" onclick="growDiv('4')" id="menu-item-4" href="#">Collaborations | |
| − | + | <span><img id="sidebar-arrow-4" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | |
| − | + | <div class="grow" id="grow-4"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#MELBOURNE" class="list-group-item" style="color: rgb(179, 179, 179);">MELBOURNE</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKUST" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKUST</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Collaborations#HONG_KONG_HKU" class="list-group-item" style="color: rgb(179, 179, 179);">HONG_KONG_HKU</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li><a class="icon icon-notebook" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Parts">Parts Registry</a></li> | |
| − | + | <li><a class="icon icon-safety" id="menu-item-5" href="https://2016.igem.org/Team:NUS_Singapore/Safety">Safety</a></li> | |
| − | + | <li><a class="icon icon-team" id="menu-item-6" href="https://2016.igem.org/Team:NUS_Singapore/Team">Team</a></li> | |
| − | + | <li><a class="icon icon-notebook" id="menu-item-7" onclick="growDiv('7')" href="https://2016.igem.org/Team:NUS_Singapore/Notebook">Notebook | |
| − | + | <span><img id="sidebar-arrow-7" src="https://static.igem.org/mediawiki/2016/c/ce/T--NUS_Singapore--scroll-down-arrow.png" style="position: relative; height: 20px; float: right;"></span></a> | |
| − | + | <div class="grow" id="grow-7"> | |
| − | + | <div class='measuringWrapper'> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group1" class="list-group-item" style="color: rgb(179, 179, 179);">I. RIOT Sensor</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group2" class="list-group-item" style="color: rgb(179, 179, 179);">II. RIOT Responder</a> | |
| − | + | <a href="https://2016.igem.org/Team:NUS_Singapore/Notebook/Group3" class="list-group-item" style="color: rgb(179, 179, 179);">III. RIOT Invader</a> | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </li> | |
| − | + | <li><a class="icon icon-acknowledgement" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Attributions">Attributions</a></li> | |
| − | + | <li><a class="icon icon-cup" id="menu-item-8" href="https://2016.igem.org/Team:NUS_Singapore/Medals">Medals</a></li> | |
| − | + | </ul> | |
| − | + | </nav> | |
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Latest revision as of 17:44, 19 October 2016
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Wet Lab Notebook: RIOT RESPONDER
Jun
2016
Overall Strategies
1 June, 2016 Milestone 1 : Overall cloning design
12 June, 2016 Milestone 2: Construction of pConst-LuxR-HA
Construct 1:
Fig 1. A. LuxR gene amplified from iGEM distribution 2016 with designed primers GEM002 and GEM005. The expected band size of LuxR with overhang is 795 bp. B.LuxR stitiched with terminater (BBa_B0015) with overlapping PCR. C. Constructed full-length RBS-pConstitutive-LuxR-HA-Terminator with expected size of 1034 bp.
July
2016
Construct 2:
Fig 2. A. LuxI gene is cloned from iGEM2016 distribution kit with primers GEM008 and GEM011. The observed band size of LuxI with overhangs is close to the expected band size of 629 bp. B.Terminator BBa_B0015 is amplified using GEM009 and 012 and stitiched with LuxI. C. Promoter-RBS fragment is synthesized and stitiched to LuxI-terminator by PCR reaction. The observed band size for full LuxI construct is close to the expected band size of 936 bp.
4 July, 2016 Milestone 3: : Construction of pLuxR-LuxI-HA
11 July, 2016 Milestone 4: Screening of pLuxR-invasin constructs
Aug
2016
1 Aug, 2016 Milestone 5: Screening of pLuxR-LLO construct
8 Aug, 2016 Milestone 6: Construction of pLuxR-GFP plasmid
