Revision as of 19:08, 19 October 2016

NOTEBOOK

Labjournal 2016

Lab Weeks

For half a year we were working in the wet lab , developed a pipetting robot and simulated the molecular dynamics of our proteins . As well we would like to highlight certain social events which helped us to further improve our team spirit.


Lab Week 1: April 18 - April 24		Hier könnte Ihr labjournal stehen!
		Meeting with the people interested in the technical part of the project, discussing the task and the possible ideas.
		modeling
		social


Lab Week 2: April 25 - Mai 1		Hier könnte Ihr labjournal stehen!
		Decision who will participate in the project and which direction we chose. 4 people forming the team and we do some research. Idea is to redesign a 3D printer
		modeling
		social


Lab Week 3: May 2 - May 8		Hier könnte Ihr labjournal stehen!
		Meeting and making a team commitment
		modeling
		social


Lab Week 4: May 9 - May 15		Hier könnte Ihr labjournal stehen!
		Brainstorm the possible ideas, discussing the found approaches. Need of more research
		modeling
		social


Lab Week 5: May 16 - May 22		Hier könnte Ihr labjournal stehen!
		Deciding the components and the programming language. Details still open.
		modeling
		social


Lab Week 6: May 23 - May 29		Hier könnte Ihr labjournal stehen!
		Made decision about the chassis, an Ultimaker2 clone. Pumping system will be a syringe pump from hackaday.io Two will look for the needed materials for the chassis, one the materials for the syringe pump and one will think about the detection system with an optic.
		modeling
		social


Lab Week 7: May 30 - June 5		Hier könnte Ihr labjournal stehen!
		robotics
		modeling
		social


Lab Week 8: June 6 - June 12		Hier könnte Ihr labjournal stehen!
		We start to design the required CADs and learn the CAD software to do so. One of us is still doing some research about the optical detection system and how to do it.
		modeling
		social


Lab Week 9: June 13 - June 19		Hier könnte Ihr labjournal stehen!
		robotics
		modeling
		social


Lab Week 10: June 20 - June 26		Hier könnte Ihr labjournal stehen!
		CADs are ready and the bill of materials is finished with the supplier information. We order the parts and talk to our workshop if they can make the needed adjustments. Our optics is chosen to be a Raspberry Pi NoIR Camera and we are using the OpenCV package to do the detection work.
		modeling
		social


Lab Week 11: June 27 - July 3		Hier könnte Ihr labjournal stehen!
		The ordered materials arrived and we started assembling the chassis. We talked to the workshop and they prepare some of the parts for us. One of our team members is preparing the OpenCV software and getting familiar with the camera
		modeling
		social


Lab Week 12: July 4 - July 10		Hier könnte Ihr labjournal stehen!
		We have some problems assembling the chassis. Some parts need to be adapted. We like to have as much active measuring area as possible. The first 3D printed parts for the syringe pump are ready and we need to redesign some parts. The optics require IR light from underneath. Next week we are discussing the details of the construction
		modeling
		social


Lab Week 13: July 11 - July 17		Hier könnte Ihr labjournal stehen!
		We will built a lightbox wit IR LEDs. To assure a homogeneous IR light we will use a diffuser plate illuminated from the sides. One team member prepares the technical drawing.
		modeling
		social


Lab Week 14: July 18 - July 24		Hier könnte Ihr labjournal stehen!
		We are working on the chassis, the syringe pump and the optics. We have first object detections from the camera and the main parts of the chassis are assembled.
		modeling
		social


Lab Week 15: July 25 - July 31		Hier könnte Ihr labjournal stehen!
		robotics
		modeling
		social


Lab Week 16: August 1 - August 7		Hier könnte Ihr labjournal stehen!
		We are waiting for the workshop to finish parts for the lightbox and the z-axis. We are working on the detection of rectangles and getting familiar with the Marlin software
		modeling
		social


Lab Week 17: August 8 - August 14		Reporter System BBa_K1976002: The mVenus-LVA-gene was synthesized by idt and amplified through PCR using the SP-amplify-fw-Rep1 (fw) and SP-amplify-rev-Rep2 (REV) oligonucleotides.
		We are playing with the stepper motors and waiting for some final parts for the x-y-axis.
		modeling
		social


Lab Week 18: August 15 - August 21		Reporter System BBa_K1976002: The PCR product was verified by agarose gel electrophoresis and subsequently purified. The mVenus amplicon was cut with EcoRI and PstI and ligated into a pSB1C3 vector using T4-ligase.
		x-y-axis is mounted but we still need to adjust everything so it is moving smoothly The z-axis need a holdering made out of aluminum blocks we have to order. In that time we working on the GUI and the Qt software
		modeling
		social


Lab Week 19: August 22 - August 28		Reporter System BBa_K1976002: The ligation product was transformed into E.coli Top 10 by heat shock transformation and the cells were spread out on a CMP agar plate.
		robotics
		modeling
		social


Lab Week 20: August 29 - September 4		Reporter System BBa_K1976002: Clones were screened with colony PCR using VF2 and VR oligonucleotides. Plasmid DNA was isolated from positive clones. Metabolic burden BBa_K1976001: The BioBrick BBa_I11020 was synthezised with a LVA‑tag and with B0034 as a ribosome binding site by IDT.
		robotics
		modeling
		social


Lab Week 21: September 5 - September 11		Reporter System BBa_K1976002: Isolated Plasmid DNA Sequence was verified by Sanger Sequencing. Metabolic burden BBa_K1976001: The LVA‑tag was deleted by PCR with the primer Del_LVA_fw and Del_LVA_rev.
		Mounted the z-axis and tuned the x-y axis. The optical software is nearly ready to use and we like to test it next week. We started connecting the electronics to an ATX power supply. The Raspberry Pi is set up and ready to use. We still have some problems with the wireless connection.
		modeling
		social


Lab Week 22: September 12 - September 18		Reporter System BBa_K1976003: The mVenus-LVA-pSB1C3 plasmid was cut with EcoRI and PstI and ligated into a T7-RBS-pSB1A3 plasmid which was cut with SpeI and PstI. Metabolic burden BBa_K1976000: The BioBrick BBa_E0240 was cut with the restriction enzymes XbaI and PstI. BBa_J61002 was cut with SpeI and PstI. The two restriction products were ligated by standard T4‑Ligation protocol. BBa_K1976001: B0034‑BBa_I11020 was cut with XbaI and PstI. BBa_J23101 was cut with SpeI and PstI. Both fragments were ligated using the T4‑Ligase by standard protocol.
		We installed the LEDs, relays and drivers for the LEDs. Sometimes they are not working properly but it seems to be a problem of the software, one is focusing on that task. X-y-Axis and z-axis movement works. We are installing the endstops to assure the steppers motors are stopping at the end of the movement.
		modeling
		social


Lab Week 23: September 19 - September 25		Reporter System BBa_K1976003: The ligation product was transformed into E.coli Top 10 by heat shock transformation and the cells were spread out on a AMP agar plate. Metabolic burden BBa_K1976000: After plasmid preparation, the plasmid was cut with XbaI and PstI. We had the attp‑site BBa_11023 flanked by the two additional bidirectional transcription terminators BBa_1001 synthesized by IDT. The attp‑site of BBa_11023 was not the original λ‑attp‑site. Instead the sequence corresponded to the attP2‑site used in the Gateway ® cloning system. The construct was cut with EcoRI and SpeI. The pSB1C3 backbone was cut with EcoRI and PstI. Together with the previously cut BBa_J61002‑BBa_E0240 construct the synthesized BBa_1001‑BBa_11023‑BBa_1001 construct was ligated in the cut pSB1C3. BBa_K1976001: The ligation product was transformed into E. coli Top 10 via heatshock.
		The GUI is on principle ready to use. Our RAMPS board is maybe broken and we buy another one. The syringe pump was tested and it works like expected.
		modeling
		social


Lab Week 24: September 26 - October 2		Reporter System BBa_K1976003: Clones were screened with colony PCR using VF2 and VR oligonucleotides. Plasmid DNA was isolated from positive clones . Metabolic burden BBa_K1976000: The attP2‑site was mutated to λ‑attp via Quick‑Change‑PCR, using the following primers: QC_attp_fw and QC_attp_rev (for more details see our primerlist). BBa_K1976001: To check whether BBa_I11020 was expressed properly a SDS‑Page was made.
		Munich sent us their syringe pump. We are now printing the additional parts and like to test it. Still working on the electronics and one LED strip needs to be soldered again. We installed our lightbox and we can clearly see the objects and have target vectors which we are using for our auto tracking later
		modeling
		social


Lab Week 25: October 3 - October 9		Reporter System BBa_K1976003: The isolated Plasmid DNA was verified by Sanger sequencing. Metabolic burden BBa_K1976000: The LVA‑tag was added by PCR with the overhang primer LVA_GFP_fw and LVA_GFP_rev.
		We assembled the Munich syringe pump and tested it. It works good and we have new ideas for our system. We gave them feedback for their design. Our robot is working but we need to fix some bugs. Also we have to adjust some parameters.
		modeling
		social


Lab Week 26: October 10 - October 16		Reporter System BBa_K1976003: Subsequently the psB1A3 vector containing brick was cut with EcoRI and PstI and ligated into a pSB1C3 vector using T4‑Ligase. Metabolic burden BBa_K1976000: For plasmid curing the final plasmid construct and the low copy backbone pSB4A5 were cut with EcoRI and PstI and then ligated. BBa_K1976001: For plasmid curing the final plasmid construct and the low copy vector pSB4K5 were cut with EcoRI and PstI and then ligated. BBa_K1976000 and BBa_K1976001 were cotransformed into E. coli Top 10. To check whether the genomic integration was successful a cPCR with attb_fw and VR primers was made.
		We are testing the functionality of the robot. Unfortunately we still have no mVenus to work with, however we can do some test with our light detection. We encapsulated the robot and can control it via network. We are finalizing the robot
		modeling
		social


Lab Week 27: October 17 - October 23		Metabolic burden Plasmid curing could not be performed due to lack of time.
		Time is running and we are working on the last details. We are exited for the Jamboree.
		modeling
		social

@@ Line 26: / Line 26: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>Meeting with the people interested in the technical part of the project, discussing the task and the possible ideas.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>Meeting with the people interested in the technical part of the project, discussing the task and the possible ideas.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 40: / Line 40: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>Decision who will participate in the project and which direction we chose.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>Decision who will participate in the project and which direction we chose.
 people forming the team and we do some research.
-Idea is to redesign a 3D printer</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+Idea is to redesign a 3D printer</p></td></tr>
-<td>robotics</td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 57: / Line 57: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>Meeting and making a team commitment</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>Meeting and making a team commitment</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 72: / Line 72: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>Brainstorm the possible ideas, discussing the found approaches.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-Need of more research</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>Brainstorm the possible ideas, discussing the found approaches.
-<td>robotics</td></tr>
+Need of more research</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 88: / Line 88: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>Deciding the components and the programming language. Details still open.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>Deciding the components and the programming language. Details still open.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 103: / Line 103: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>Made decision about the chassis, an Ultimaker2 clone. Pumping system will be a syringe pump from hackaday.io
+<tr><td>
-Two will look for the needed materials for the chassis, one the materials for the syringe pump and one will think about the detection system with an optic.</p>
 </td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>Made decision about the chassis, an Ultimaker2 clone. Pumping system will be a syringe pump from hackaday.io
+Two will look for the needed materials for the chassis, one the materials for the syringe pump and one will think about the detection system with an optic.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 135: / Line 135: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>We start to design the required CADs and learn the CAD software to do so. One of us is still doing some research about the optical detection system and how to do it.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>We start to design the required CADs and learn the CAD software to do so. One of us is still doing some research about the optical detection system and how to do it.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 165: / Line 165: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>CADs are ready and the bill of materials is finished with the supplier information. We order the parts and talk to our workshop if they can make the needed adjustments.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-Our optics is chosen to be a Raspberry Pi NoIR Camera and we are using the OpenCV package to do the detection work.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>CADs are ready and the bill of materials is finished with the supplier information. We order the parts and talk to our workshop if they can make the needed adjustments.
-<td>robotics</td></tr>
+Our optics is chosen to be a Raspberry Pi NoIR Camera and we are using the OpenCV package to do the detection work.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 181: / Line 181: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>The ordered materials arrived and we started assembling the chassis. We talked to the workshop and they prepare some of the parts for us.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-One of our team members is preparing the OpenCV software and getting familiar with the camera</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>The ordered materials arrived and we started assembling the chassis. We talked to the workshop and they prepare some of the parts for us.
-<td>robotics</td></tr>
+One of our team members is preparing the OpenCV software and getting familiar with the camera</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 197: / Line 197: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>We have some problems assembling the chassis. Some parts need to be adapted. We like to have as much active measuring area as possible.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>We have some problems assembling the chassis. Some parts need to be adapted. We like to have as much active measuring area as possible.
 The first 3D printed parts for the syringe pump are ready and we need to redesign some parts.
-The optics require IR light from underneath. Next week we are discussing the details of the construction</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+The optics require IR light from underneath. Next week we are discussing the details of the construction</p></td></tr>
-<td>robotics</td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 214: / Line 214: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>We will built a lightbox wit IR LEDs. To assure a homogeneous IR light we will use a diffuser plate illuminated from the sides.  One team member prepares the technical drawing.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>We will built a lightbox wit IR LEDs. To assure a homogeneous IR light we will use a diffuser plate illuminated from the sides.  One team member prepares the technical drawing.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 229: / Line 229: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>We are working on the chassis, the syringe pump and the optics.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-We have first object detections from the camera and the main parts of the chassis are assembled. </p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>We are working on the chassis, the syringe pump and the optics.
-<td>robotics</td></tr>
+We have first object detections from the camera and the main parts of the chassis are assembled. </p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 260: / Line 260: @@
 <td><p>Hier könnte Ihr labjournal stehen!</p>
 </td></tr>
-<tr><td><p>We are waiting for the workshop to finish parts for the lightbox and the z-axis.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-We are working on the detection of rectangles and getting familiar with the Marlin software</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>We are waiting for the workshop to finish parts for the lightbox and the z-axis.
-<td>robotics</td></tr>
+We are working on the detection of rectangles and getting familiar with the Marlin software</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 274: / Line 274: @@
 <div class="labWeek"><h5>Lab Week 17: <div class="week_date"> August 8 - August 14</div></h5></div></td>
 <td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/b/b5/T--TU_Darmstadt--iconLab3.png"/></td>
 <td><p><h6>Reporter System</h6>
 <b>BBa&#95;K1976002:</b><br>
@@ Line 279: / Line 280: @@
 </td></tr>
-<tr><td><p>We are playing with the stepper motors and waiting for some final parts for the x-y-axis.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>We are playing with the stepper motors and waiting for some final parts for the x-y-axis.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 297: / Line 298: @@
 </td></tr>
-<tr><td><p>x-y-axis is mounted but we still need to adjust everything so it is moving smoothly
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-The z-axis need a holdering made out of aluminum blocks we have to order. In that time we working on the GUI and the Qt software</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>x-y-axis is mounted but we still need to adjust everything so it is moving smoothly
-<td>robotics</td></tr>
+The z-axis need a holdering made out of aluminum blocks we have to order. In that time we working on the GUI and the Qt software</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 358: / Line 359: @@
 The LVA&#8209;tag was deleted by PCR with the primer Del&#95;LVA&#95;fw and Del&#95;LVA&#95;rev.</p>
 </td></tr>
-<tr><td><p>Mounted the z-axis and tuned the x-y axis. The optical software is nearly ready to use and we like to test it next week.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>Mounted the z-axis and tuned the x-y axis. The optical software is nearly ready to use and we like to test it next week.
 We started connecting the electronics to an ATX power supply.
-The Raspberry Pi is set up and ready to use. We still have some problems with the wireless connection.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+The Raspberry Pi is set up and ready to use. We still have some problems with the wireless connection.</p></td></tr>
-<td>robotics</td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 389: / Line 390: @@
 Both fragments were ligated using the T4&#8209;Ligase by standard protocol.</p>
 </td></tr>
-<tr><td><p>We installed the LEDs, relays and drivers for the LEDs. Sometimes they are not working properly but it seems to be a problem of the software, one is focusing on that task.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-X-y-Axis and z-axis movement works. We are installing the endstops to assure the steppers motors are stopping at the end of the movement. </p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>We installed the LEDs, relays and drivers for the LEDs. Sometimes they are not working properly but it seems to be a problem of the software, one is focusing on that task.
-<td>robotics</td></tr>
+X-y-Axis and z-axis movement works. We are installing the endstops to assure the steppers motors are stopping at the end of the movement. </p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 418: / Line 419: @@
 <b>BBa&#95;K1976001:</b><br>The ligation product was transformed into <i>E.&nbsp;coli</i> Top&nbsp;10 via heatshock. </p>
 </td></tr>
-<tr><td><p>The GUI is on principle ready to use. Our RAMPS board is maybe broken and we buy another one. The syringe pump was tested and it works like expected. </p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>The GUI is on principle ready to use. Our RAMPS board is maybe broken and we buy another one. The syringe pump was tested and it works like expected. </p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 444: / Line 445: @@
 To check whether BBa&#95;I11020 was expressed properly a SDS&#8209;Page was made.</p>
 </td></tr>
-<tr><td><p>Munich sent us their syringe pump. We are now printing the additional parts and like to test it.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>Munich sent us their syringe pump. We are now printing the additional parts and like to test it.
 Still working on the electronics and one LED strip needs to be soldered again.
-We installed our lightbox and we can clearly see the objects and have target vectors which we are using for our auto tracking later</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+We installed our lightbox and we can clearly see the objects and have target vectors which we are using for our auto tracking later</p></td></tr>
-<td>robotics</td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 467: / Line 468: @@
 The LVA&#8209;tag was added by PCR with the overhang primer LVA&#95;GFP&#95;fw and LVA&#95;GFP&#95;rev.</p>
 </td></tr>
-<tr><td><p>We assembled the Munich syringe pump and tested it. It works good and we have new ideas for our system.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>We assembled the Munich syringe pump and tested it. It works good and we have new ideas for our system.
 We gave them feedback for their design.
-Our robot is working but we need to fix some bugs. Also we have to adjust some parameters.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+Our robot is working but we need to fix some bugs. Also we have to adjust some parameters.</p></td></tr>
-<td>robotics</td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 496: / Line 497: @@
 To check whether the genomic integration was successful a cPCR with attb&#95;fw and VR primers was made.</p>
 </td></tr>
-<tr><td><p>We are testing the functionality of the robot. Unfortunately we still have no mVenus to work with, however we can do some test with our light detection.
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<td><p>We are testing the functionality of the robot. Unfortunately we still have no mVenus to work with, however we can do some test with our light detection.
 We encapsulated the robot and can control it via network.
-We are finalizing the robot</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+We are finalizing the robot</p></td></tr>
-<td>robotics</td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>
@@ Line 515: / Line 516: @@
 </p>
 </td></tr>
-<tr><td><p>Time is running and we are working on the last details. We are exited for the Jamboree.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
-<td>robotics</td></tr>
+<td><p>Time is running and we are working on the last details. We are exited for the Jamboree.</p></td></tr>
 <tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 <td>modeling</td></tr>

Difference between revisions of "Team:TU Darmstadt/Notebook"

Revision as of 19:08, 19 October 2016

NOTEBOOK

Labjournal 2016

Lab Week 1: April 18 - April 24

Lab Week 2: April 25 - Mai 1

Lab Week 3: May 2 - May 8

Lab Week 4: May 9 - May 15

Lab Week 5: May 16 - May 22

Lab Week 6: May 23 - May 29

Lab Week 7: May 30 - June 5

Lab Week 8: June 6 - June 12

Lab Week 9: June 13 - June 19

Lab Week 10: June 20 - June 26

Lab Week 11: June 27 - July 3

Lab Week 12: July 4 - July 10

Lab Week 13: July 11 - July 17

Lab Week 14: July 18 - July 24

Lab Week 15: July 25 - July 31

Lab Week 16: August 1 - August 7

Lab Week 17: August 8 - August 14

Reporter System

Lab Week 18: August 15 - August 21

Reporter System

Lab Week 19: August 22 - August 28

Reporter System

Lab Week 20: August 29 - September 4

Reporter System

Metabolic burden

Lab Week 21: September 5 - September 11

Reporter System

Metabolic burden

Lab Week 22: September 12 - September 18

Reporter System

Metabolic burden

Lab Week 23: September 19 - September 25

Reporter System

Metabolic burden

Lab Week 24: September 26 - October 2

Reporter System

Metabolic burden

Lab Week 25: October 3 - October 9

Reporter System

Metabolic burden

Lab Week 26: October 10 - October 16

Reporter System

Metabolic burden

Lab Week 27: October 17 - October 23

Metabolic burden

Lab Week 1:
April 18 - April 24

Lab Week 2:
April 25 - Mai 1

Lab Week 3:
May 2 - May 8

Lab Week 4:
May 9 - May 15

Lab Week 5:
May 16 - May 22

Lab Week 6:
May 23 - May 29

Lab Week 7:
May 30 - June 5

Lab Week 8:
June 6 - June 12

Lab Week 9:
June 13 - June 19

Lab Week 10:
June 20 - June 26

Lab Week 11:
June 27 - July 3

Lab Week 12:
July 4 - July 10

Lab Week 13:
July 11 - July 17

Lab Week 14:
July 18 - July 24

Lab Week 15:
July 25 - July 31

Lab Week 16:
August 1 - August 7

Lab Week 17:
August 8 - August 14

Lab Week 18:
August 15 - August 21

Lab Week 19:
August 22 - August 28

Lab Week 20:
August 29 - September 4

Lab Week 21:
September 5 - September 11

Lab Week 22:
September 12 - September 18

Lab Week 23:
September 19 - September 25

Lab Week 24:
September 26 - October 2

Lab Week 25:
October 3 - October 9

Lab Week 26:
October 10 - October 16

Lab Week 27:
October 17 - October 23