Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pVP-EL222P"
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<div class="thelanguage"> | <div class="thelanguage"> | ||
<li><p style="font-size: 18px;">pVP-EL222 checked with KOD polymerase plasmid PCR with remixed primers</p></li> | <li><p style="font-size: 18px;">pVP-EL222 checked with KOD polymerase plasmid PCR with remixed primers</p></li> | ||
| − | <li><p style="font-size: 18px;">pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and | + | <li><p style="font-size: 18px;">pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p> | ||
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<div class="technology">9/7</div> | <div class="technology">9/7</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and | + | <li><p style="font-size: 18px;">pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p> | ||
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<div class="technology">9/8</div> | <div class="technology">9/8</div> | ||
<div class="thelanguage"> | <div class="thelanguage"> | ||
| − | <li><p style="font-size: 18px;">pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and | + | <li><p style="font-size: 18px;">pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)</p></li> |
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 14); return false">[Collapse]</a></p> | <p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 14); return false">[Collapse]</a></p> | ||
Revision as of 19:21, 19 October 2016
pVP-EL222P
pVP-EL222P/h1>
Received plasmid pVP-EL222 from University of Texas Southwestern
pVP-EL222 resuspended (sample from University of Texas Southwestern)
pVP-EL222 transformed into competent cells
pVP-EL222 did 3 in 1
pVP-EL222 extracted from transformed cultures and checked with restriction digestion (EcoRI andXhoI)
pVP-EL222 plasmid stored and transformed cultures cryopreserved
pVP-EL222 amplified with plasmid PCR (contaminated NC)
pVP-EL222 checked with plasmid PCR with brand new reagents (success, no contamination detected)
pVP-EL222 amplified with plasmid PCR
pVP-EL222 digested with SpeI and BamHI)
pVP-EL222 amplified with PCR
pVP-EL222 amplified with PCR than digested
pVP-EL222 checked separately with plasmid PCR with redesigned primers
pVP-EL222 checked with plasmid PCR due to multiple failed ligations
pVP-EL222 checked with restriction digestion due to multiple failed ligations
pVP-EL222 checked with Taq polymerase plasmid PCR with remixed primers
pVP-EL222 checked with KOD polymerase plasmid PCR with remixed primers
pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)
pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)
pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)
