Difference between revisions of "Team:BroadRun-Baltimore/Parts"

 
(15 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<html>
 
<html>
  
 +
<style>
 +
table, th, td {
 +
    border: 1px solid black;
 +
    border-collapse: collapse;
 +
}
  
 +
 +
th, td {
 +
    padding: 15px;
 +
}
 +
 +
th {
 +
    text-align: left;
 +
    font-size: 20px;
 +
}
 +
 +
td
 +
{
 +
text-align: left;
 +
font-size: 17px;
 +
 +
</style>
  
  
Line 9: Line 30:
 
<div class="column full_size">
 
<div class="column full_size">
  
−
<br><br><br><br><br><br><br><br><br><br><br>
+
<br><br><br>
−
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
<h1>Parts</h1>
 +
 
 +
<hr>
 +
<br>
 +
<h2>New Parts Submitted to the Registry in 2016 </h2>
 +
 
 +
<table style="width:100%">
 +
 
 +
  <tr>
 +
    <th>Part Name</th>
 +
    <th>Part Number</th>
 +
  </tr>
 +
 +
<tr>
 +
    <td>Penicillium alpha amylase w/ no promoter</td>
 +
    <td>BBa_K2185003</td>
 +
  </tr>
 +
 
 +
<tr>
 +
    <td>B.cereus beta amylase w/ no promoter</td>
 +
    <td>BBa_K2185005</td>
 +
  </tr>
 +
 
 +
<tr>
 +
    <td>S.ferax alpha amylase w/ cyc promoter</td>
 +
    <td>BBa_K2185007</td>
 +
  </tr>
 +
</table>
 +
<br>
 +
 
 +
<h3>Composite Part 1: BBa_K2185003</h3>
 +
<p1>Promoterless</p1>
 +
<p1>Kozak sequence (BBa_K165002)</p1>
 +
<p1>Mating Factor Secretion Tag (BBa_K792002)</p1>
 +
<p1>Alpha amylase coding sequence from the fungus Penicillium </p1>
 +
<p1>ADH1 Terminator (Part BBa_K392003)</p1>
 +
 
 +
<h3>Composite Part 2: BBa_K2185005</h3>
 +
<p1>Promoterless</p1>
 +
<p1>Kozak sequence (Part BBa_K165002)</p1>
 +
<p1>Mating Factor Secretion Tag (BBa_K792002)</p1>
 +
<p1>Beta amylase coding sequence from Bacillus cereus</p1>
 +
<p1>ADH1 Terminator (Part BBa_K392003)</p1>
 +
 
 +
 
 +
<h3>Composite Part 3: BBa_K2185007</h3>
 +
<p1>pCyc medium promoter (BBa_I766555)</p1>
 +
<p1>Kozak sequence (Part BBa_K165002)</p1>
 +
<p1>Mating Factor Secretion Tag (BBa_K792002)</p1>
 +
<p1>Alpha amylase coding sequence from Saprolegnia ferax</p1>
 +
<p1>ADH1 Terminator (Part BBa_K392003)</p1>
 +
<br><br>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<h4><u>Description of Basic Parts:</u> </h4>
 +
 
 +
<p1><b>ADH1 Terminator</b> - This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the DNA sequence.</p1>
 +
 
 +
 
 +
<br><br>
 +
<p1><b>Kozak Sequence</b> - This part initiates translation from eukaryotic mRNA and is placed between a promoter and coding sequence to facilitate translation. </p1>
 +
<br><br>
 +
 
 +
 
 +
<p1>
 +
<b>pCyc medium promoter</b> - This is a medium expression level constitutive promoter. It directs the cell to transcribe the DNA sequence constantly. </p1>
 +
<br><br>
 +
 
 +
<p1>
 +
<b>Mating Factor Alpha Secretion Sequence</b> - This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein. </p1>
 +
<br><br>
 +
 
 +
<p1>
 +
<b>Penicillium Alpha Amylase</b> - Obtained from the fungus Penicillium, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides. </p1>
 +
<br><br>
 +
 
 +
<p1>
 +
<b>S.ferax Alpha Amylase</b> - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides. </p1>
 +
<br><br>
  
 +
<p1>
 +
<b>B.cereus Beta Amylase</b> - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses beta bonds of large, beta-linked polysaccharides, such as cellulose and some starches, thus degrading the polysaccharides into monosaccharides and disaccharides. </p1>
 +
<br><br>
  
−
</body>
 
  
 +
<p1>In addition to the 3 new parts submitted this year, we also improved a previous part, from last year. To see how we improved the characterization of this part, please see <a href="http://parts.igem.org/Part:BBa_K1871002">here</a>. </p1>
 
</html>
 
</html>

Latest revision as of 19:49, 19 October 2016

.MathJax nobr>span.math>span{border-left-width:0 !important};








Parts


New Parts Submitted to the Registry in 2016

Part Name Part Number
Penicillium alpha amylase w/ no promoter BBa_K2185003
B.cereus beta amylase w/ no promoter BBa_K2185005
S.ferax alpha amylase w/ cyc promoter BBa_K2185007

Composite Part 1: BBa_K2185003

Promoterless Kozak sequence (BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from the fungus Penicillium ADH1 Terminator (Part BBa_K392003)

Composite Part 2: BBa_K2185005

Promoterless Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Beta amylase coding sequence from Bacillus cereus ADH1 Terminator (Part BBa_K392003)

Composite Part 3: BBa_K2185007

pCyc medium promoter (BBa_I766555) Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from Saprolegnia ferax ADH1 Terminator (Part BBa_K392003)

Description of Basic Parts:

ADH1 Terminator - This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the DNA sequence.

Kozak Sequence - This part initiates translation from eukaryotic mRNA and is placed between a promoter and coding sequence to facilitate translation.

pCyc medium promoter - This is a medium expression level constitutive promoter. It directs the cell to transcribe the DNA sequence constantly.

Mating Factor Alpha Secretion Sequence - This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.

Penicillium Alpha Amylase - Obtained from the fungus Penicillium, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

S.ferax Alpha Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

B.cereus Beta Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses beta bonds of large, beta-linked polysaccharides, such as cellulose and some starches, thus degrading the polysaccharides into monosaccharides and disaccharides.

In addition to the 3 new parts submitted this year, we also improved a previous part, from last year. To see how we improved the characterization of this part, please see here.