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+ | <div class="column full_size"> | ||
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+ | <br><br><br> | ||
+ | <h1>Parts</h1> | ||
+ | |||
+ | <hr> | ||
+ | <br> | ||
+ | <h2>New Parts Submitted to the Registry in 2016 </h2> | ||
+ | |||
+ | <table style="width:100%"> | ||
+ | |||
+ | <tr> | ||
+ | <th>Part Name</th> | ||
+ | <th>Part Number</th> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Penicillium alpha amylase w/ no promoter</td> | ||
+ | <td>BBa_K2185003</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>B.cereus beta amylase w/ no promoter</td> | ||
+ | <td>BBa_K2185005</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>S.ferax alpha amylase w/ cyc promoter</td> | ||
+ | <td>BBa_K2185007</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <br> | ||
+ | |||
+ | <h3>Composite Part 1: BBa_K2185003</h3> | ||
+ | <p1>Promoterless</p1> | ||
+ | <p1>Kozak sequence (BBa_K165002)</p1> | ||
+ | <p1>Mating Factor Secretion Tag (BBa_K792002)</p1> | ||
+ | <p1>Alpha amylase coding sequence from the fungus Penicillium </p1> | ||
+ | <p1>ADH1 Terminator (Part BBa_K392003)</p1> | ||
+ | |||
+ | <h3>Composite Part 2: BBa_K2185005</h3> | ||
+ | <p1>Promoterless</p1> | ||
+ | <p1>Kozak sequence (Part BBa_K165002)</p1> | ||
+ | <p1>Mating Factor Secretion Tag (BBa_K792002)</p1> | ||
+ | <p1>Beta amylase coding sequence from Bacillus cereus</p1> | ||
+ | <p1>ADH1 Terminator (Part BBa_K392003)</p1> | ||
+ | |||
+ | |||
+ | <h3>Composite Part 3: BBa_K2185007</h3> | ||
+ | <p1>pCyc medium promoter (BBa_I766555)</p1> | ||
+ | <p1>Kozak sequence (Part BBa_K165002)</p1> | ||
+ | <p1>Mating Factor Secretion Tag (BBa_K792002)</p1> | ||
+ | <p1>Alpha amylase coding sequence from Saprolegnia ferax</p1> | ||
+ | <p1>ADH1 Terminator (Part BBa_K392003)</p1> | ||
+ | <br><br> | ||
+ | |||
+ | |||
+ | |||
+ | |||
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+ | |||
+ | <h4><u>Description of Basic Parts:</u> </h4> | ||
+ | |||
+ | <p1><b>ADH1 Terminator</b> - This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the DNA sequence.</p1> | ||
+ | |||
+ | |||
+ | <br><br> | ||
+ | <p1><b>Kozak Sequence</b> - This part initiates translation from eukaryotic mRNA and is placed between a promoter and coding sequence to facilitate translation. </p1> | ||
+ | <br><br> | ||
+ | <p1> | ||
+ | <b>pCyc medium promoter</b> - This is a medium expression level constitutive promoter. It directs the cell to transcribe the DNA sequence constantly. </p1> | ||
+ | <br><br> | ||
+ | <p1> | ||
+ | <b>Mating Factor Alpha Secretion Sequence</b> - This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein. </p1> | ||
+ | <br><br> | ||
+ | <p1> | ||
+ | <b>Penicillium Alpha Amylase</b> - Obtained from the fungus Penicillium, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides. </p1> | ||
+ | <br><br> | ||
− | < | + | <p1> |
− | + | <b>S.ferax Alpha Amylase</b> - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides. </p1> | |
+ | <br><br> | ||
− | < | + | <p1> |
+ | <b>B.cereus Beta Amylase</b> - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses beta bonds of large, beta-linked polysaccharides, such as cellulose and some starches, thus degrading the polysaccharides into monosaccharides and disaccharides. </p1> | ||
+ | <br><br> | ||
− | |||
+ | <p1>In addition to the 3 new parts submitted this year, we also improved a previous part, from last year. To see how we improved the characterization of this part, please see <a href="http://parts.igem.org/Part:BBa_K1871002">here</a>. </p1> | ||
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Latest revision as of 19:49, 19 October 2016
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Parts
New Parts Submitted to the Registry in 2016
Part Name | Part Number |
---|---|
Penicillium alpha amylase w/ no promoter | BBa_K2185003 |
B.cereus beta amylase w/ no promoter | BBa_K2185005 |
S.ferax alpha amylase w/ cyc promoter | BBa_K2185007 |