Difference between revisions of "Team:BroadRun-Baltimore/Parts"

 
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<br><br><br>
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<h1>Parts</h1>
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<hr>
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<br>
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<h2>New Parts Submitted to the Registry in 2016 </h2>
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<table style="width:100%">
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  <tr>
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    <th>Part Name</th>
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    <th>Part Number</th>
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  </tr>
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<tr>
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    <td>Penicillium alpha amylase w/ no promoter</td>
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    <td>BBa_K2185003</td>
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  </tr>
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<tr>
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    <td>B.cereus beta amylase w/ no promoter</td>
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    <td>BBa_K2185005</td>
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  </tr>
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<tr>
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    <td>S.ferax alpha amylase w/ cyc promoter</td>
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    <td>BBa_K2185007</td>
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<h3>Composite Part 1: BBa_K2185003</h3>
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<p1>Promoterless</p1>
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<p1>Kozak sequence (BBa_K165002)</p1>
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<p1>Mating Factor Secretion Tag (BBa_K792002)</p1>
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<p1>Alpha amylase coding sequence from the fungus Penicillium </p1>
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<p1>ADH1 Terminator (Part BBa_K392003)</p1>
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<h3>Composite Part 2: BBa_K2185005</h3>
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<p1>Promoterless</p1>
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<p1>Kozak sequence (Part BBa_K165002)</p1>
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<p1>Mating Factor Secretion Tag (BBa_K792002)</p1>
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<p1>Beta amylase coding sequence from Bacillus cereus</p1>
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<p1>ADH1 Terminator (Part BBa_K392003)</p1>
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<h3>Composite Part 3: BBa_K2185007</h3>
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<p1>pCyc medium promoter (BBa_I766555)</p1>
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<p1>Kozak sequence (Part BBa_K165002)</p1>
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<p1>Mating Factor Secretion Tag (BBa_K792002)</p1>
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<p1>Alpha amylase coding sequence from Saprolegnia ferax</p1>
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<p1>ADH1 Terminator (Part BBa_K392003)</p1>
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<br><br>
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<h4><u>Description of Basic Parts:</u> </h4>
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<p1><b>ADH1 Terminator</b> - This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the DNA sequence.</p1>
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<br><br>
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<p1><b>Kozak Sequence</b> - This part initiates translation from eukaryotic mRNA and is placed between a promoter and coding sequence to facilitate translation. </p1>
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<br><br>
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<p1>
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<b>pCyc medium promoter</b> - This is a medium expression level constitutive promoter. It directs the cell to transcribe the DNA sequence constantly. </p1>
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<br><br>
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<p1>
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<b>Mating Factor Alpha Secretion Sequence</b> - This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein. </p1>
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<br><br>
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<p1>
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<b>Penicillium Alpha Amylase</b> - Obtained from the fungus Penicillium, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.  </p1>
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<br><br>
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<p1>
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<b>S.ferax Alpha Amylase</b> - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.  </p1>
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<br><br>
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<p1>
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<b>B.cereus Beta Amylase</b> - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses beta bonds of large, beta-linked polysaccharides, such as cellulose and some starches, thus degrading the polysaccharides into monosaccharides and disaccharides. </p1>
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<br><br>
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<p1>In addition to the 3 new parts submitted this year, we also improved a previous part, from last year. To see how we improved the characterization of this part, please see <a href="http://parts.igem.org/Part:BBa_K1871002">here</a>. </p1>
 
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Latest revision as of 19:49, 19 October 2016

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Parts


New Parts Submitted to the Registry in 2016

Part Name Part Number
Penicillium alpha amylase w/ no promoter BBa_K2185003
B.cereus beta amylase w/ no promoter BBa_K2185005
S.ferax alpha amylase w/ cyc promoter BBa_K2185007

Composite Part 1: BBa_K2185003

Promoterless Kozak sequence (BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from the fungus Penicillium ADH1 Terminator (Part BBa_K392003)

Composite Part 2: BBa_K2185005

Promoterless Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Beta amylase coding sequence from Bacillus cereus ADH1 Terminator (Part BBa_K392003)

Composite Part 3: BBa_K2185007

pCyc medium promoter (BBa_I766555) Kozak sequence (Part BBa_K165002) Mating Factor Secretion Tag (BBa_K792002) Alpha amylase coding sequence from Saprolegnia ferax ADH1 Terminator (Part BBa_K392003)

Description of Basic Parts:

ADH1 Terminator - This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the DNA sequence.

Kozak Sequence - This part initiates translation from eukaryotic mRNA and is placed between a promoter and coding sequence to facilitate translation.

pCyc medium promoter - This is a medium expression level constitutive promoter. It directs the cell to transcribe the DNA sequence constantly.

Mating Factor Alpha Secretion Sequence - This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.

Penicillium Alpha Amylase - Obtained from the fungus Penicillium, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

S.ferax Alpha Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, thus degrading the polysaccharides into monosaccharides and disaccharides.

B.cereus Beta Amylase - Obtained from the fungus Saprolegnia ferax, α-Amylase is an enzyme that hydrolyses beta bonds of large, beta-linked polysaccharides, such as cellulose and some starches, thus degrading the polysaccharides into monosaccharides and disaccharides.

In addition to the 3 new parts submitted this year, we also improved a previous part, from last year. To see how we improved the characterization of this part, please see here.