<p id="pp"> The protocol was improved by using a latin rectangle arrangement in another 96 well plate. A lab robot was used to pipette out the corresponding cultures to their wells according to our preset programme linked to a descrambling spreadsheet. The Latin rectangle is designed so that no sample gets pipetted more than once in any row or column. The order in which the samples get arranged was worked out using the random number function of Microsoft Excel. Sterile LB was loaded into peripheral wells to lessen edge effects which can skew results due to high evaporation at the periphery. These adaptations aim to provide more accurate data by reducing plate effects, samples are spread randomly around the plate so as to minimise differences in results due to irregularities in plate temperature.</p>

<p id="pp"> The protocol was improved by using a latin rectangle arrangement in another 96 well plate. A lab robot was used to pipette out the corresponding cultures to their wells according to our preset programme linked to a descrambling spreadsheet. The Latin rectangle is designed so that no sample gets pipetted more than once in any row or column. The order in which the samples get arranged was worked out using the random number function of Microsoft Excel. Sterile LB was loaded into peripheral wells to lessen edge effects which can skew results due to high evaporation at the periphery. These adaptations aim to provide more accurate data by reducing plate effects, samples are spread randomly around the plate so as to minimise differences in results due to irregularities in plate temperature.</p>

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<div class="col-xs-6"><span class="caption" style="padding: 5px 30% 5px 30%;">Fig. 3 Latin rectangle, schematic representation of a 10 sample culture layout on a 96 well-plate. The different colours and patterns represent the aliquots that were measured. The peripheral wells in red represent sterile LB media in order to reduce edge effects.</span></div>

<div class="col-xs-6"><span class="caption" style="padding: 5px 30% 5px 30%;">Fig. 3 Latin rectangle, schematic representation of a 10 sample culture layout on a 96 well-plate. The different colours and patterns represent the aliquots that were measured. The peripheral wells in red represent sterile LB media in order to reduce edge effects.</span></div>

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<p id="pp">The measurements were taken using the same calibration settings as before on the Tecan plate reader every hour for 6 hours. The plate was placed into mini shaking incubator at 37°C between plate readings. A scratch was noticed on the plate lid and was replaced. This lid was cold compared to the incubated plate and so condensation formed this could have affected the results. Placed in incubator briefly to warm up. The results were compiled into graphs using the iGEM Interlab excel document (Fig. 4 and Fig. 5).</p>

<p id="pp">The measurements were taken using the same calibration settings as before on the Tecan plate reader every hour for 6 hours. The plate was placed into mini shaking incubator at 37°C between plate readings. A scratch was noticed on the plate lid and was replaced. This lid was cold compared to the incubated plate and so condensation formed this could have affected the results. Placed in incubator briefly to warm up. The results were compiled into graphs using the iGEM Interlab excel document (Fig. 4 and Fig. 5).</p>

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