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− | + | <html lang="en-US"> | |
− | <html> | + | <head> |
+ | <title>IGEM-IIT Kharagpur- Part</title> | ||
+ | <meta charset="utf-8"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/part_bootstrap?action=raw&ctype=text/css" /> | ||
+ | <link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/part_font?action=raw&ctype=text/css" /> | ||
+ | <link href="https://fonts.googleapis.com/css?family=Ubuntu" rel="stylesheet"> | ||
+ | <link href="https://fonts.googleapis.com/css?family=Droid+Sans" rel="stylesheet"> | ||
+ | |||
+ | |||
+ | |||
+ | <!--=== Other CSS files ===--> | ||
+ | |||
+ | <link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/part_vegas?action=raw&ctype=text/css" /> | ||
+ | |||
+ | <link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/part_bxslider?action=raw&ctype=text/css" /> | ||
+ | |||
+ | <!--=== Main Stylesheets ===--> | ||
+ | <link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/part_style?action=raw&ctype=text/css" /> | ||
+ | <link rel="stylesheet" href="https://2016.igem.org/Template:IIT_Kharagpur/part_responsive?action=raw&ctype=text/css" /> | ||
+ | |||
+ | <!--=== Color Scheme, three colors are available red.css, orange.css and gray.css ===--> | ||
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+ | padding-top: 15px; | ||
− | |||
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− | |||
− | |||
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+ | body, html { | ||
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− | |||
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+ | .dropdown-content a:hover {background-color: #b04545} | ||
+ | |||
+ | .dropdown:hover .dropdown-content { | ||
+ | display: block; | ||
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+ | |||
+ | .dropdown:hover .dropbtn { | ||
+ | background-color: #3e8e41; | ||
+ | } | ||
+ | |||
+ | .completefooter{ | ||
+ | padding-top: 5px; | ||
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+ | .list-group-item { | ||
+ | |||
+ | border: 1px solid #344560; | ||
+ | } | ||
+ | .test-images{ | ||
+ | width: 100%; | ||
+ | background-color: white; | ||
+ | padding-top: 10px; | ||
+ | } | ||
+ | .igem-logo-top{ | ||
+ | width: 55px; | ||
+ | height: auto; | ||
+ | |||
+ | float: left; | ||
+ | position: relative; | ||
+ | right: 12px; | ||
+ | } | ||
+ | .center_icon{ | ||
+ | width: 200px; | ||
+ | height: auto; | ||
+ | |||
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+ | margin-left:-7.5em; | ||
+ | margin-top: -1%; | ||
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+ | |||
+ | </style> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </head> | ||
+ | <body > | ||
+ | <!--=== Preloader section starts ===--> | ||
+ | <section id="preloader"> | ||
+ | <div class="loading-circle fa-spin"></div> | ||
+ | </section> | ||
+ | <!--=== Preloader section Ends ===--> | ||
+ | |||
+ | <!--=== Header section Starts ===--> | ||
+ | <div id="header" class="header-section"> | ||
+ | <!-- sticky-bar Starts--> | ||
+ | <div class="sticky-bar-wrap"> | ||
+ | <div class="sticky-section"> | ||
+ | <div id="topbar-hold" class="nav-hold container"><a href="https://2016.igem.org/Team:IIT_Kharagpur"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/thumb/8/86/T--IIT_Kharagpur--logo.png/180px-T--IIT_Kharagpur--logo.png" class="igem-logo-top"></a> | ||
+ | <nav class="navbar" role="navigation"> | ||
+ | <div class="navbar-header"> | ||
+ | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target=".navbar-responsive-collapse"> | ||
+ | <span class="sr-only">Toggle navigation</span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | <!--=== Site Name ===--> | ||
+ | <a class="site-name navbar-brand" href="https://2016.igem.org/Team:IIT_Kharagpur"><span>IIT</span>Kharagpur</a> | ||
+ | </div> | ||
+ | |||
+ | <!-- Main Navigation menu Starts --> | ||
+ | <div class="collapse navbar-collapse navbar-responsive-collapse"> | ||
+ | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li class="current dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur"><img src="https://static.igem.org/mediawiki/2016/1/13/T--IIT_Kharagpur--home_icon.png" class="icon"> Home</a> | ||
+ | |||
+ | </li> | ||
+ | <li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Project"><img src="https://static.igem.org/mediawiki/2016/5/55/T--IIT_Kharagpur--project_icon.png" class="icon">Project</a> | ||
+ | <div class="dropdown-content"> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Notebook" class="submenuhover">Notebook</a> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Protocol" class="submenuhover">Protocol</a> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Data_page" class="submenuhover">Data Page</a> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Safety" class="submenuhover">Safety</a> | ||
+ | </div> | ||
+ | |||
+ | </li> | ||
+ | <li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Team"><img src="https://static.igem.org/mediawiki/2016/c/c1/T--IIT_Kharagpur--team_icon.png" class="icon">Team</a> | ||
+ | <div class="dropdown-content"> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Team" class="submenuhover">Members</a> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Collaborations" class="submenuhover">Collaboration</a> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Attributions" class="submenuhover">Attribution</a> | ||
+ | |||
+ | </div> | ||
+ | </li > | ||
+ | <li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/9/9f/T--IIT_Kharagpur--practice_icon.png" class="icon">Practices</a> | ||
+ | <div class="dropdown-content"> | ||
+ | <a href="#" class="submenuhover">Survey</a> | ||
+ | <a href="#" class="submenuhover">Workshop</a> | ||
+ | <a href="#" class="submenuhover">Interview</a> | ||
+ | <a href="#" class="submenuhover">Meet ups</a> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </li> | ||
+ | <li class="dropdown"><a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts"><img src="https://static.igem.org/mediawiki/2016/7/76/T--IIT_Kharagpur--part_icon.png" class="icon">Part</a> | ||
+ | <div class="dropdown-content"> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts" class="submenuhover">Composite parts</a> | ||
+ | <a href="https://2016.igem.org/Team:IIT_Kharagpur/Parts" class="submenuhover">Part collection </a> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | <li><a href="https://2016.igem.org/Team:IIT_Kharagpur/Interlab_Study"><img src="https://static.igem.org/mediawiki/2016/d/df/T--IIT_Kharagpur--interlab_icon.png" class="icon">Interlab</a></li> | ||
+ | <li><a href="blog.html"><img src="https://static.igem.org/mediawiki/2016/5/5e/T--IIT_Kharagpur--blog_icon.png" class="icon">Blog</a></li> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | <!-- Main Navigation menu ends--> | ||
+ | |||
+ | |||
+ | </nav> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- sticky-bar Ends--> | ||
+ | <!--=== Header section Ends ===--> | ||
+ | |||
+ | <!--=== Home Section Starts ===--> | ||
+ | <div id="section-home" class="home-section-wrap center"> | ||
+ | <div class="section-overlay"></div> | ||
+ | <div class="topcontainer home"> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="home_content"> | ||
+ | |||
+ | <h1 class="well-come" style="font-family:Dosis;">PARTS</h1> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/7/76/T--IIT_Kharagpur--part_icon.png" class="center_icon"> | ||
+ | |||
+ | <!-- <div class="col-md-8 col-md-offset-2 tabledown"> | ||
+ | <p class="intro-message" style="font-family:Dosis; font-size:20px; text-align:center;">HARD WORK JUST LIKE JUST MA AND PA TAUGHT US</p> | ||
+ | </div> --> | ||
+ | <div class="home-buttons"> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!--=== Home Section Ends ===--> | ||
+ | </div> | ||
+ | <div class="col-md-12 bodysection"> | ||
+ | <div class="col-md-10 col-md-offset-1 center section-titler section-title"> | ||
+ | <h3>Overview</h3> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-lg-7 col-md-7 col-sm-12 justify safety-content"> | ||
+ | <p style="font-family:Dosis; font-size:20px;"> | ||
+ | Integrating various aspects, iGEM, above all, is a struggle to turn your idea into reality. Ultimately, this implies creating the precious parts. The parts are created by joining biobricks. This year, we have used the standard assembly protocols in order to ligate the various necessary biobricks. | ||
+ | However, we could not achieve our vision, and this year we will be submitting two intermediate parts, and one composite part. | ||
+ | </p> | ||
+ | |||
+ | </div> | ||
+ | <div class="col-lg-4 col-md-4 col-sm-12 col-md-offset-1 justify safety-content" > | ||
+ | <img src="https://static.igem.org/mediawiki/2016/6/61/T--IIT_Kharagpur--globe.png"><br><br> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <div class="col-md-12 bodysection"> | ||
+ | <div class="col-md-12 col-md-offser-1"> | ||
+ | <table class="table table-bordered"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>SL.No.</th> | ||
+ | <th>Name/Part No.</th> | ||
+ | <th>Type</th> | ||
+ | <th>Design</th> | ||
+ | <th>Length(bp)</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tr> | ||
+ | <td>1.</td> | ||
+ | <td> | ||
+ | BBa_K2163000</td> | ||
+ | <td>Composite</td> | ||
+ | <td>CFP+HIV+YFP</td> | ||
+ | <td>1499</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>2.</td> | ||
+ | <td>BBa_S05365</td> | ||
+ | <td> | ||
+ | Intermediate | ||
+ | </td> | ||
+ | <td>CFP+HIV</td> | ||
+ | <td>773</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>3.</td> | ||
+ | <td>BBa_S05366</td> | ||
+ | <td> | ||
+ | Intermediate | ||
+ | </td> | ||
+ | <td>HIV+YFP</td> | ||
+ | <td>768</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div><br><br> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="col-md-12 bodysection"> | ||
+ | <div class="col-md-10 col-md-offset-1 center section-titler section-title"> | ||
+ | <h3>PARTS</h3> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-lg-7 col-md-7 col-sm-12 justify safety-content"> | ||
+ | <h4><b>1. BBa_K2163000 | ||
+ | </b></h4> | ||
+ | <p style="font-family:Dosis; font-size:20px;"> | ||
+ | This is a composite part, comprising of Cyan Fluorescent Protein (CFP), HIV Aspartyl Protease Specific Cleavage Site, and Yellow Fluorescent Protein (YFP). This part functions as the core of FRET detection system. As long as the cleavage site is intact, the FRET pair is active, while cleavage of the site leads to inactivation of the FRET pair. When attached with OmpA at the C terminal, the entire system is presented on the cell surface. | ||
+ | </p><br><br><br> | ||
+ | |||
+ | </div><br> | ||
+ | <div class="col-lg-5 col-md-5 col-sm-12 justify safety-content" > | ||
+ | <img src="https://static.igem.org/mediawiki/2016/7/78/T--IIT_Kharagpur--cfp_hiv_yfp.png"><br><br><br> | ||
+ | </div> | ||
+ | <div class="col-lg-7 col-md-7 col-sm-12 justify safety-content"> | ||
+ | <h4><b>2. BBa_S05365 | ||
+ | </b></h4> | ||
+ | <p style="font-family:Dosis; font-size:20px;"> | ||
+ | This is one of the two submitted intermediate parts. It consists of Cyan Fluorescent Protein (CFP) and HIV Aspartyl Protease Specific Cleavage Site. | ||
+ | </p> | ||
+ | <br><br><br> | ||
+ | |||
+ | </div><br> | ||
+ | <div class="col-lg-5 col-md-5 col-sm-12 justify safety-content" > | ||
+ | <img src="https://static.igem.org/mediawiki/2016/d/d9/T--IIT_Kharagpur--cfp_hiv.png"><br><br><br> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-lg-7 col-md-7 col-sm-12 justify safety-content"> | ||
+ | <h4><b>3. BBa_S05366 | ||
+ | </b></h4> | ||
+ | <p style="font-family:Dosis; font-size:20px;"> | ||
+ | This is the other intermediate part. It is made up of the biobricks coding for HIV Aspartyl Protease Specific Cleavage Site and Yellow Fluorescent Protein (YFP). | ||
+ | </p><br><br><br> | ||
+ | |||
+ | </div><br> | ||
+ | <div class="col-lg-5 col-md-5 col-sm-12 justify safety-content" > | ||
+ | <img src="https://static.igem.org/mediawiki/2016/4/43/T--IIT_Kharagpur--hiv_yfp.png"><br><br><br> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <!-- | ||
+ | <div class="col-md-12 bodysection"> | ||
+ | <div class="col-md-10 col-md-offset-1 center section-titler section-title"> | ||
+ | <h3>References</h3> | ||
+ | </div> | ||
+ | <div class="col-lg-12 col-md-12 col-sm-12 justify safety-content"> | ||
+ | <p style="font-family:Dosis; font-size:20px;"> | ||
+ | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum<br><br></div> | ||
+ | <div class="col-md-10 col-md-offset-1 center section-titler section-title"> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> --> | ||
+ | |||
+ | |||
+ | |||
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Latest revision as of 20:18, 19 October 2016
PARTS
![](https://static.igem.org/mediawiki/2016/7/76/T--IIT_Kharagpur--part_icon.png)
Overview
Integrating various aspects, iGEM, above all, is a struggle to turn your idea into reality. Ultimately, this implies creating the precious parts. The parts are created by joining biobricks. This year, we have used the standard assembly protocols in order to ligate the various necessary biobricks. However, we could not achieve our vision, and this year we will be submitting two intermediate parts, and one composite part.
![](https://static.igem.org/mediawiki/2016/6/61/T--IIT_Kharagpur--globe.png)
SL.No. | Name/Part No. | Type | Design | Length(bp) |
---|---|---|---|---|
1. | BBa_K2163000 | Composite | CFP+HIV+YFP | 1499 |
2. | BBa_S05365 | Intermediate | CFP+HIV | 773 |
3. | BBa_S05366 | Intermediate | HIV+YFP | 768 |
PARTS
1. BBa_K2163000
This is a composite part, comprising of Cyan Fluorescent Protein (CFP), HIV Aspartyl Protease Specific Cleavage Site, and Yellow Fluorescent Protein (YFP). This part functions as the core of FRET detection system. As long as the cleavage site is intact, the FRET pair is active, while cleavage of the site leads to inactivation of the FRET pair. When attached with OmpA at the C terminal, the entire system is presented on the cell surface.
![](https://static.igem.org/mediawiki/2016/7/78/T--IIT_Kharagpur--cfp_hiv_yfp.png)
2. BBa_S05365
This is one of the two submitted intermediate parts. It consists of Cyan Fluorescent Protein (CFP) and HIV Aspartyl Protease Specific Cleavage Site.
![](https://static.igem.org/mediawiki/2016/d/d9/T--IIT_Kharagpur--cfp_hiv.png)
3. BBa_S05366
This is the other intermediate part. It is made up of the biobricks coding for HIV Aspartyl Protease Specific Cleavage Site and Yellow Fluorescent Protein (YFP).
![](https://static.igem.org/mediawiki/2016/4/43/T--IIT_Kharagpur--hiv_yfp.png)