Difference between revisions of "Team:Exeter/Parts"

 
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#title{
 
#title{
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/*Makes side pictures on banner invisible on small screens*/
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     <div>
 
     <div>
 
         <div class="collapse navbar-collapse" id="myNavbar">
 
         <div class="collapse navbar-collapse" id="myNavbar">
−
<ul class="nav navbar-nav">
+
<ul class="nav navbar-nav">
−
<li><div id="links" style="margin:0;" class="dropdown">
+
<li><div id="links_drop" class="dropdown">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 
     <span style="margin-bottom:4px;">Lab</span>
 
     <span style="margin-bottom:4px;">Lab</span>
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<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Project">Lab Project</a></li>
 
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Project">Lab Project</a></li>
 
     <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Labbook">Lab Book</a></li>
 
     <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Labbook">Lab Book</a></li>
 +
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Safety">Safety</a></li>
  
 
   </ul>
 
   </ul>
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<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Parts">Parts</a></li>
 
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Parts">Parts</a></li>
 
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Team">Team</a></li>
 
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Team">Team</a></li>
−
<li ><a id="links" href="https://2016.igem.org/Team:Exeter/Interlab">InterLab</a></li>
+
<li><a id="links" href="https://2016.igem.org/Team:Exeter/Interlab">InterLab</a></li>
−
<li><div id="links" style="margin:0;" class="dropdown">
+
<li><div id="links_drop" class="dropdown">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 
     <span style="margin-bottom:4px;">Human Practices</span>
 
     <span style="margin-bottom:4px;">Human Practices</span>
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   </button>
 
   </button>
 
   <ul class="dropdown-menu" style="background:#e8e8e8;margin-left:25px;" aria-labelledby="dropdownMenu1">   
 
   <ul class="dropdown-menu" style="background:#e8e8e8;margin-left:25px;" aria-labelledby="dropdownMenu1">   
−
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Human_Practices">Human Practices Homepage</a></li>
+
    <li><a id="links" style="margin:10px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Integrated_Practices">Integrated</a></li>
−
    <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Integrated_Practices">Integrated</a></li>
+
<li><a id="links" style="background:none;line-height:0.7vh;margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Engagement">Public Engagement<br /><br /><br /> & Education</a></li><li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Log">Log</a></li>
−
<li><a id="links" style="background:none;line-height:0.7vh;margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Engagement">Public Engagement<br /><br /><br /> & Education</a></li>
+
−
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Log">Log</a></li>
+
  
 
   </ul>
 
   </ul>
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<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Attributions">Attributions</a></li>
 
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Attributions">Attributions</a></li>
  
−
<li><div id="links" style="margin:0;" class="dropdown">
+
<li><div id="links_drop" class="dropdown">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 
     <span style="margin-bottom:4px;">Awards</span>
 
     <span style="margin-bottom:4px;">Awards</span>
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   <ul class="dropdown-menu" style="background:#e8e8e8;margin-left:25px;" aria-labelledby="dropdownMenu1">
 
   <ul class="dropdown-menu" style="background:#e8e8e8;margin-left:25px;" aria-labelledby="dropdownMenu1">
 
    
 
    
−
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Awards">Medals</a></li>
+
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Awards">Awards</a></li>
−
<li><span style="margin:10px 0 30px 2px;padding:0;">Special pages</span></li>
+
<li><span style="margin:10px 0 30px 2px;padding:0;"><u>Special pages</u></span></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Silver">HP Silver</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Silver">HP Silver</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Gold">HP Gold</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Gold">HP Gold</a></li>
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<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Model">Models</a></li>
 
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Model">Models</a></li>
 
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Collaborations">Collaborations</a></li>
 
<li ><a id="links"href="https://2016.igem.org/Team:Exeter/Collaborations">Collaborations</a></li>
 +
 +
 +
 
</ul>
 
</ul>
 
<ul class="nav navbar-nav navbar-right">
 
<ul class="nav navbar-nav navbar-right">
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<!--Contains all information of banner in the middle-->
 
<!--Contains all information of banner in the middle-->
 
<!--of the two outer images-->
 
<!--of the two outer images-->
−
<div class="col-xs-12 col-sm-8 subdiv_banner middle">
+
<div class="col-xs-12 col-sm-8 subdiv_banner middle">
 
<!--Use bootstrap to add links using all 12 columns-->
 
<!--Use bootstrap to add links using all 12 columns-->
 
<!--For each link please give double the coloumns for when-->
 
<!--For each link please give double the coloumns for when-->
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<!--Give span class "oneline" or "twoline" depending on how llong the section text is-->
 
<!--Give span class "oneline" or "twoline" depending on how llong the section text is-->
−
<a href="#section_1" class="banner_link col-xs-6 col-sm-3"><span class="oneline">Description</span></a>
+
<a href="#section_1" class="banner_link col-xs-6 col-sm-3"><span class="oneline">KillerRed</span></a>
−
<a href="#section_2" class="banner_link col-xs-6 col-sm-3"><span class="oneline">KillerRed</span></a>
+
<a href="#section_2" class="banner_link col-xs-6 col-sm-3"><span class="oneline">KillerOrange</span></a>
−
<a href="#section_3" class="banner_link col-xs-6 col-sm-3"><span class="oneline">KillerOrange</span></a>
+
<a href="#section_3" class="banner_link col-xs-6 col-sm-3"><span class="oneline">Lysozyme</span></a>
−
<a href="#section_4" class="banner_link col-xs-6 col-sm-3"><span class="oneline">Lysozyme</span></a>
+
                                <a href="#section_4" class="banner_link col-xs-6 col-sm-3"><span class="oneline">DNase</span></a>
−
</div>
+
</div>
 +
 
<!--Left picture (the teal line on left)-->
 
<!--Left picture (the teal line on left)-->
 
<div class="col-sm-2 subdiv_banner right">
 
<div class="col-sm-2 subdiv_banner right">
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</div>
 
</div>
 
<div>
 
<div>
−
<a id="Section_link" href="#section_1" style="display:block;margin:34vh auto 0 auto;width:14px;"><span style="color:#47BCC2;font-size: 25px;" class="glyphicon glyphicon-menu-down" aria-hidden="true"></span></a>
+
 
</div>
 
</div>
 
</div>
 
</div>
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<div id="section_1" class="link_fix"></div>
 
<div id="section_1" class="link_fix"></div>
 
<div id="contentTitle">
 
<div id="contentTitle">
−
Description
+
KillerRed
−
 
+
 
</div>
 
</div>
  
−
                 <p id="pp">The aim of our project was to extensively test the viability of different of kill switches across an array of variables. We focused on the ones that have already been used by previous teams (Pekin 2014, Oxford 2015) as well as a KillerRed homologue KillerOrange. This will allow future teams to more accurately chose a kill switch that suits their project the best as we have providing better understanding on the durability of the different kill switches as well as their efficiency. </p>
+
                <h6>Name</h6>
−
                 <p id="pp">We submitted a total of X new parts all of which are BioBrick compatible and codon optimised for E. coli strain K12. See list below for more details. </p>
+
 
 +
                 <p id="pp">pT7- <i>E. coli</i> optimised - KillerRed (EOKR)</p>
 +
 
 +
                <h6>Description</h6>
 +
 
 +
                <p id="pp">KillerRed is a red fluorescent protein that generates reactive oxygen species after illumination with light between the wavelengths of 540-580 nm[1].</p>
 +
 
 +
                <p id="pp">We further characterised this kill switch by illuminating induced cultures 24 hours after induction with IPTG as well as uninduced cultures. After 6 hours in the light box CFU’s were counted to determine if the kill switch was successful. This was also reproduced on cultures grown in a ministat for 120 and 168 hours to test how long the kill switch remains functional. The full protocol can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_4">here</a> and the results can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a>. </p>
 +
 
 +
                 <p id="pp">The mechanism by which KillerRed kills cells isn’t fully understood yet</p>
 +
 
 +
                <p id="pp">Here we are submitting KillerRed as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
 +
 
 +
                <p id="pp">The sequence for KillerRed protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914002">(BBa_K1141002)</a></p>
 +
 
 +
                <h6>Biobrick Code</h6>
 +
 
 +
                <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914003">BBa_K1914003</a></p>
 +
 
 +
<br>
 +
<br>
 +
 
 +
                <div class="col-xs-12" style="padding:0;margin:0;">
 +
                    <img src="https://static.igem.org/mediawiki/2016/1/1a/T--Exeter--parts-KR_png.png" style="max-width:50%;margin:auto;display:block;">
 +
<br>
 +
<br>
  
 
<div>
 
<div>
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<a id="Section_link" href="#section_2" style="display:block;margin:20px auto 0 auto;width:14px;"><span style="color:#47BCC2;font-size: 25px;" class="glyphicon glyphicon-menu-down" aria-hidden="true"></span></a>
 
<a id="Section_link" href="#section_2" style="display:block;margin:20px auto 0 auto;width:14px;"><span style="color:#47BCC2;font-size: 25px;" class="glyphicon glyphicon-menu-down" aria-hidden="true"></span></a>
 
</div>
 
</div>
−
</div>
+
 
<div class="col-xs-12 div_content">
 
<div class="col-xs-12 div_content">
 
<div id="section_2" class="link_fix"></div>
 
<div id="section_2" class="link_fix"></div>
 
<div id="contentTitle">
 
<div id="contentTitle">
−
KillerRed
+
KillerOrange
 
</div>
 
</div>
 +
<h6>Name:</h6>
  
−
                 <h6>Name</h6>
+
                 <p id="pp">pT7- <i>E. coli</i> optimised - KillerOrange (EOKO)</p>
  
−
                 <p id="pp">pT7- E. coli optimised - KillerRed (EOKR)</p>
+
                 <h6>Description:</h6>
  
−
                 <h6>Description</h6>
+
                 <p id="pp">KillerOrange is a mutant of the fluorescent protein KillerRed (BBa_K1141002, BBa_K1491015) activated by blue and green light. It carries a tryptophan-based chromophore that is novel for photosensitizers [2]. </p>
  
−
                 <p id="pp">KillerRed is a red fluorescent protein that generates reactive oxygen species after illumination with light between the wavelengths of 540-580nm[1].</p>
+
                 <p id="pp">KillerOrange has an excitation maximum of 512 nm and emission maximum at 555 nm and its absorbance spectrum has two peaks, at 455 and 514 nm. <p>
  
−
                 <p id="pp">We further characterised this kill switch by illuminating induced cultures 24 hours after induction with IPTG as well as uninduced cultures. After 6 hours in the light box CFU’s were counted to determine if the kill switch was successful. This was also reproduced on cultures grown in a ministat for 120 and 168 hours to test how long the kill switch remains functional. The full protocol can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_5">here</a> and the results can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a>. </p>
+
                 <p id="pp">The mechanism by which KillerOrange kills cells isn’t fully understood yet. However, it is believed that KillerOrange's ability to generate reactive oxygen species depends on a water-filler channel reaching the chromophore area from the end cap of the ß-barrel [3].</p>
  
−
                 <p id="pp">The mechanism by which KillerRed kills cells isn’t fully understood yet</p>
+
               
 +
                 <p id="pp">We characterised this part in the same way as KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_4">protocol</a>), and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p>
  
−
                 <p id="pp">Here we are submitting KillerRed as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
+
                 <p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
  
−
                 <p id="pp">The sequence for KillerRed protein coding region can be found here (BBa_K1141002)</p>
+
                 <p id="pp">The sequence for KillerOrange protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914000">(BBa_K1914000)</a></p>
  
−
                 <h6>Biobrick Code</h6>
+
                 <h6> Biobrick Code </h6>
 +
                <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914001">BBa_K1914001</a></p>
 +
 
 +
<br>
 +
<br>
 +
 
 +
                <div class="col-xs-12" style="padding:0;margin:0;">
 +
                    <img src="https://static.igem.org/mediawiki/2016/2/21/T--Exeter--parts-KO_png.png" style="max-width:50%;margin:auto;display:block;">
 +
<br>
 +
<br>
 +
               
 +
                </div>
  
−
                <p id="pp">BBa_K1914003</p>
 
  
 
<div>
 
<div>
 
<a id="Section_link" href="#section_3" style="display:block;margin:20px auto 0 auto;width:14px;"><span style="color:#47BCC2;font-size: 25px;" class="glyphicon glyphicon-menu-down" aria-hidden="true"></span></a>
 
<a id="Section_link" href="#section_3" style="display:block;margin:20px auto 0 auto;width:14px;"><span style="color:#47BCC2;font-size: 25px;" class="glyphicon glyphicon-menu-down" aria-hidden="true"></span></a>
 
</div>
 
</div>
−
</div>
+
 
<div class="col-xs-12 div_content">
 
<div class="col-xs-12 div_content">
 
<div id="section_3" class="link_fix"></div>
 
<div id="section_3" class="link_fix"></div>
 
<div id="contentTitle">
 
<div id="contentTitle">
−
KillerOrange
+
Lysozyme
 
</div>
 
</div>
 +
<h6>Name:</h6>
  
−
                <h6>Name:</h6>
+
                 <p id="pp">pT7 Lysozyme  <i>E. coli</i> codon optimised, signal peptide, flag tag</p>
−
 
+
−
                 <p id="pp">pT7- E. coli optimised - KillerOrange (EOKO)</p>
+
  
 
                 <h6>Description:</h6>
 
                 <h6>Description:</h6>
  
−
                 <p id="pp">KillerOrange is a mutant of the fluorescent protein KillerRed (BBa_K1141002, BBa_K1491015) activated by blue and green light. It carries a tryptophan-based chromophore that is novel for photosensitizers [2]. </p>
+
                 <p id="pp">Lysozyme  from chicken egg white, attacks the cell wall in bacteria by hydrolysing the ß-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine of peptidoglycan [4].</p>
  
−
                 <p id="pp">KillerOrange has an excitation maximum of 512 nm and emission maximum at 555 nm and its absorbance spectrum has two peaks, at 455 and 514 nm. <p>
+
                 <p id="pp"> </p>
 +
                <p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_4">protocol</a>)and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p>
  
−
                 <p id="pp">It is believed that was confers KillerOrange's ability to generate reactive oxygen species depends on a water-filler channel reaching the chromophore area from the end cap of the β-barrel [3].</p>
+
                 <p id="pp">Here we are submitting lysozyme as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
  
−
                 <p id="pp">The mechanism by which KillerOrange kills cells isn’t fully understood yet[3]. </p>
+
                 <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and exchanged the native signal peptide for one which is <i>E. coli</i> specific and directs Lysozyme to the periplasm [5]. The sequence for Lysozyme protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914004">(BBa_K1914004)</a></p>
  
−
                 <p id="pp">We characterised this part in the same way as KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_5">protocol</a>), and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p>
+
                 <h6> Biobrick Code </h6>
  
−
                 <p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
+
                 <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914005">BBa_K1914005</a></p>
  
−
                <p id="pp">The sequence for KillerOrange protein coding region can be found here (BBa_K1914000)</p>
+
<br>
 +
<br>
  
−
                <h6> Biobrick Code </h6>
+
<div class="col-xs-12" style="padding:0;margin:0;">
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                <p id="pp">BBa_K1914001</p>
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                    <img src="https://static.igem.org/mediawiki/2016/c/c5/T--Exeter--parts-LYSO_png.png" style="max-width:50%;margin:auto;display:block;">
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<div id="contentTitle">
 
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LysozymeC
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DNase
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<h6>Name:</h6>
  
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                <h6>Name:</h6>
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                 <p id="pp">DNase</p>
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                 <p id="pp">LysozymeC</p>
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                 <h6>Description:</h6>
 
                 <h6>Description:</h6>
  
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                 <p id="pp">Lysozyme C from chicken egg white, attacks the cell wall in bacteria by hydrolysing the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine of peptidoglycan [4].</p>
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                 <p id="pp"> DNase was designed [6] as a DNA degrading kill switch aiming to prevent cross contamination of DNA</p>
  
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                 <p id="pp"> We are submitting this Lysozyme C as a composite part under a T7 promoter, an Elowitz ribosome binding site (biobrick code) and a double terminator (BBa_B0015). We have codon optimised the protein coding region, added a FLAG tag and signal peptide directing Lysozyme to the periplasm. The sequence for just Lysozyme C can be found here (biobrick code).</p>
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                <p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_5">protocol</a>)and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p>
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                 <p id="pp"> A composite part containing DNase could not be created. This could be due to the need for a tightly regulating promoter to prevent DNase protein being produced before induction and destroying DNA.</p>
  
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                <p id="pp">Here we are submitting lysozymeC as a composite part under a T7 promoter (code), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
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                <p id="pp">The sequence for LysozymeC protein coding region can be found here (BBa_K1914004)</p>
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                    <img src="https://static.igem.org/mediawiki/2016/0/06/T--Exeter--parts-DNase_png.png" style="max-width:50%;margin:auto;display:block;">
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                 <h6> Biobrick Code </h6>
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<style>
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ul{
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list-style-image:none;
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}
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.wrap ul{
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padding-top:20px;
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padding-right:40px;
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padding-left:80px;
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font-size:150%;
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}
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.wrap ul li {
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    /* Text color */
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    color: #333;
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    list-style-type: none;
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}
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.wrap ul li:before {
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    /* Unicode bullet symbol */
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    content: '\25AA';
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    /* Bullet color */
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    color: #47BCC2;
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    padding-right: 0.5em;
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}
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ol{
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padding-top:20px;
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padding-right:40px;
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padding-left:80px;
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font-size:150%;
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}
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ol li {
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    /* Text color */
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    color: #333;
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    list-style-type: none;
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}
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ol li {
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    list-style-type: none;
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    counter-increment: list;
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    position: relative;
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}
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ol li:before {
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    content: counter(list) ".";
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    position: absolute;
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    left: -2.5em;
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    width: 2em;
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    text-align: right;
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    color: #47BCC2;
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}
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</style>
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                 <h5>References</h5>
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<ol style="font-size:100%;">
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                    <li>Takemoto, K., Matsuda, T., Sakai, N., Fu, D., Noda, M., Uchiyama, S., Kotera, I., Arai, Y., Horiuchi, M., Fukui, K. and Ayabe, T., 2013. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Scientific reports, 3.
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</li>
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                    <li>Sarkisyan, K.S., Zlobovskaya, O.A., Gorbachev, D.A., Bozhanova, N.G., Sharonov, G.V., Staroverov, D.B., Egorov, E.S., Ryabova, A.V., Solntsev, K.M., Mishin, A.S. and Lukyanov, K.A., 2015. KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. PloS one,10(12), p.e0145287.
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</li>
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                    <li>Pletnev, S., Gurskaya, N.G., Pletneva, N.V., Lukyanov, K.A., Chudakov, D.M., Martynov, V.I., Popov, V.O., Kovalchuk, M.V., Wlodawer, A., Dauter, Z. and Pletnev, V., 2009. Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed. Journal of Biological Chemistry, 284(46), pp.32028-32039.
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</li>
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                    <li>Blake, C.C.F., Koenig, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., 1965. Structure of hen egg-white lysozyme: a three-dimensional Fourier synthesis at 2 Å resolution. Nature, 206(4986), pp.757-761.
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</li>
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                    <li>Fischer, B., Perry, B., Phillips, G., Sumner, I. and Goodenough, P., 1993. Physiological consequence of expression of soluble and active hen egg white lysozyme in Escherichia coli. Applied Microbiology and Biotechnology, 39, pp.537–540.
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</li>
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                    <li>Chen, C.Y., Lu, S.C. and Liao, T.H.,1998. Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli: purification and characterization of the recombinant enzyme. Gene, 206, pp.181–184.
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</li>
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                </ol>
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                <p id="pp">BBa_K1914005</p>
 
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Latest revision as of 20:24, 19 October 2016