Difference between revisions of "Team:Exeter/Parts"

 
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     <div>
 
     <div>
 
         <div class="collapse navbar-collapse" id="myNavbar">
 
         <div class="collapse navbar-collapse" id="myNavbar">
−
<ul class="nav navbar-nav">
+
<ul class="nav navbar-nav">
 
<li><div id="links_drop" class="dropdown">
 
<li><div id="links_drop" class="dropdown">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
 
   <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true">
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<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Project">Lab Project</a></li>
 
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Project">Lab Project</a></li>
 
     <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Labbook">Lab Book</a></li>
 
     <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Labbook">Lab Book</a></li>
 +
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Safety">Safety</a></li>
  
 
   </ul>
 
   </ul>
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<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Awards">Awards</a></li>
 
<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Awards">Awards</a></li>
−
<li><span style="margin:10px 0 30px 2px;padding:0;">Special pages</span></li>
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<li><span style="margin:10px 0 30px 2px;padding:0;"><u>Special pages</u></span></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Silver">HP Silver</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Silver">HP Silver</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Gold">HP Gold</a></li>
 
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Gold">HP Gold</a></li>
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<!--Contains all information of banner in the middle-->
 
<!--Contains all information of banner in the middle-->
 
<!--of the two outer images-->
 
<!--of the two outer images-->
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<div class="col-xs-12  col-sm-9 subdiv_banner middle">
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<div class="col-xs-12  col-sm-8 subdiv_banner middle">
 
<!--Use bootstrap to add links using all 12 columns-->
 
<!--Use bootstrap to add links using all 12 columns-->
 
<!--For each link please give double the coloumns for when-->
 
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<a href="#section_2" class="banner_link col-xs-6 col-sm-3"><span class="oneline">KillerOrange</span></a>
 
<a href="#section_2" class="banner_link col-xs-6 col-sm-3"><span class="oneline">KillerOrange</span></a>
 
<a href="#section_3" class="banner_link col-xs-6 col-sm-3"><span class="oneline">Lysozyme</span></a>
 
<a href="#section_3" class="banner_link col-xs-6 col-sm-3"><span class="oneline">Lysozyme</span></a>
 +
                                <a href="#section_4" class="banner_link col-xs-6 col-sm-3"><span class="oneline">DNase</span></a>
 +
</div>
 
 
 
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<!--Left picture (the teal line on left)-->
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                 <h6>Name</h6>
 
                 <h6>Name</h6>
  
−
                 <p id="pp">pT7- E. coli optimised - KillerRed (EOKR)</p>
+
                 <p id="pp">pT7- <i>E. coli</i> optimised - KillerRed (EOKR)</p>
  
 
                 <h6>Description</h6>
 
                 <h6>Description</h6>
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                 <p id="pp">Here we are submitting KillerRed as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
 
                 <p id="pp">Here we are submitting KillerRed as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
  
−
                 <p id="pp">The sequence for KillerRed protein coding region can be found here (BBa_K1141002)</p>
+
                 <p id="pp">The sequence for KillerRed protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914002">(BBa_K1141002)</a></p>
  
 
                 <h6>Biobrick Code</h6>
 
                 <h6>Biobrick Code</h6>
  
−
                 <p id="pp">BBa_K1914003</p>
+
                 <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914003">BBa_K1914003</a></p>
 +
 
 +
<br>
 +
<br>
  
 
                 <div class="col-xs-12" style="padding:0;margin:0;">
 
                 <div class="col-xs-12" style="padding:0;margin:0;">
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<h6>Name:</h6>
 
<h6>Name:</h6>
  
−
                 <p id="pp">pT7- E. coli optimised - KillerOrange (EOKO)</p>
+
                 <p id="pp">pT7- <i>E. coli</i> optimised - KillerOrange (EOKO)</p>
  
 
                 <h6>Description:</h6>
 
                 <h6>Description:</h6>
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                 <p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
 
                 <p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
  
−
                 <p id="pp">The sequence for KillerOrange protein coding region can be found here (BBa_K1914000)</p>
+
                 <p id="pp">The sequence for KillerOrange protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914000">(BBa_K1914000)</a></p>
  
 
                 <h6> Biobrick Code </h6>
 
                 <h6> Biobrick Code </h6>
−
                 <p id="pp">BBa_K1914001</p>
+
                 <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914001">BBa_K1914001</a></p>
 +
 
 +
<br>
 +
<br>
  
 
                 <div class="col-xs-12" style="padding:0;margin:0;">
 
                 <div class="col-xs-12" style="padding:0;margin:0;">
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<h6>Name:</h6>
 
<h6>Name:</h6>
  
−
                 <p id="pp">pT7 Lysozyme  E.coli codon optimised, signal peptide, flag tag</p>
+
                 <p id="pp">pT7 Lysozyme  <i>E. coli</i> codon optimised, signal peptide, flag tag</p>
  
 
                 <h6>Description:</h6>
 
                 <h6>Description:</h6>
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                 <p id="pp">Here we are submitting lysozyme as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
 
                 <p id="pp">Here we are submitting lysozyme as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p>
  
−
                 <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and exchanged the native signal peptide for one which is E. coli specific and directs Lysozyme to the periplasm. The sequence for Lysozyme protein coding region can be found here (BBa_K1914004)</p>
+
                 <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and exchanged the native signal peptide for one which is <i>E. coli</i> specific and directs Lysozyme to the periplasm [5]. The sequence for Lysozyme protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914004">(BBa_K1914004)</a></p>
  
 
                 <h6> Biobrick Code </h6>
 
                 <h6> Biobrick Code </h6>
  
−
                 <p id="pp">BBa_K1914005</p>
+
                 <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914005">BBa_K1914005</a></p>
 +
 
 +
<br>
 +
<br>
  
 
<div class="col-xs-12" style="padding:0;margin:0;">
 
<div class="col-xs-12" style="padding:0;margin:0;">
 
                     <img src="https://static.igem.org/mediawiki/2016/c/c5/T--Exeter--parts-LYSO_png.png" style="max-width:50%;margin:auto;display:block;">
 
                     <img src="https://static.igem.org/mediawiki/2016/c/c5/T--Exeter--parts-LYSO_png.png" style="max-width:50%;margin:auto;display:block;">
 +
 +
 
<br>
 
<br>
 
<br>
 
<br>
 +
 +
<div>
 +
 +
<a id="Section_link" href="#section_4" style="display:block;margin:20px auto 0 auto;width:14px;"><span style="color:#47BCC2;font-size: 25px;" class="glyphicon glyphicon-menu-down" aria-hidden="true"></span></a>
 +
</div>
 +
 +
<div class="col-xs-12 div_content">
 +
<div id="section_4" class="link_fix"></div>
 +
<div id="contentTitle">
 +
DNase
 +
</div>
 +
<h6>Name:</h6>
 +
 +
                <p id="pp">DNase</p>
 +
 +
                <h6>Description:</h6>
 +
 +
                <p id="pp"> DNase was designed [6] as a DNA degrading kill switch aiming to prevent cross contamination of DNA</p>
 +
 +
               
 +
                <p id="pp"> A composite part containing DNase could not be created. This could be due to the need for a tightly regulating promoter to prevent DNase protein being produced before induction and destroying DNA.</p>
 +
 +
<br>
 +
<br>
 +
 +
<div class="col-xs-12" style="padding:0;margin:0;">
 +
                    <img src="https://static.igem.org/mediawiki/2016/0/06/T--Exeter--parts-DNase_png.png" style="max-width:50%;margin:auto;display:block;">
 +
<br>
 +
<br>
 +
 
<style>
 
<style>
 
ul{
 
ul{
Line 809: Line 853:
 
</li>
 
</li>
 
                     <li>Blake, C.C.F., Koenig, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., 1965. Structure of hen egg-white lysozyme: a three-dimensional Fourier synthesis at 2 Å resolution. Nature, 206(4986), pp.757-761.
 
                     <li>Blake, C.C.F., Koenig, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., 1965. Structure of hen egg-white lysozyme: a three-dimensional Fourier synthesis at 2 Å resolution. Nature, 206(4986), pp.757-761.
 +
</li>
 +
                    <li>Fischer, B., Perry, B., Phillips, G., Sumner, I. and Goodenough, P., 1993. Physiological consequence of expression of soluble and active hen egg white lysozyme in Escherichia coli. Applied Microbiology and Biotechnology, 39, pp.537–540.
 +
</li>
 +
                    <li>Chen, C.Y., Lu, S.C. and Liao, T.H.,1998. Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli: purification and characterization of the recombinant enzyme. Gene, 206, pp.181–184.
 
</li>
 
</li>
 
                 </ol>
 
                 </ol>

Latest revision as of 20:24, 19 October 2016