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<div class="collapse navbar-collapse" id="myNavbar"> | <div class="collapse navbar-collapse" id="myNavbar"> | ||
− | + | <ul class="nav navbar-nav"> | |
<li><div id="links_drop" class="dropdown"> | <li><div id="links_drop" class="dropdown"> | ||
<button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true"> | <button class="dropdown-toggle" type="button" id="dropdownMenu1" data-toggle="dropdown" aria-haspopup="true" aria-expanded="true"> | ||
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<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Project">Lab Project</a></li> | <li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Project">Lab Project</a></li> | ||
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Labbook">Lab Book</a></li> | <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Labbook">Lab Book</a></li> | ||
+ | <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
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<li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Awards">Awards</a></li> | <li><a id="links" style="margin:10px 0 30px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/Awards">Awards</a></li> | ||
− | <li><span style="margin:10px 0 30px 2px;padding:0;">Special pages</span></li> | + | <li><span style="margin:10px 0 30px 2px;padding:0;"><u>Special pages</u></span></li> |
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Silver">HP Silver</a></li> | <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Silver">HP Silver</a></li> | ||
<li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Gold">HP Gold</a></li> | <li><a id="links" style="margin:30px 0 10px 2px;padding:0;font-size:1.8vh;" href="https://2016.igem.org/Team:Exeter/HP/Gold">HP Gold</a></li> | ||
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<!--Contains all information of banner in the middle--> | <!--Contains all information of banner in the middle--> | ||
<!--of the two outer images--> | <!--of the two outer images--> | ||
− | <div class="col-xs-12 col-sm- | + | <div class="col-xs-12 col-sm-8 subdiv_banner middle"> |
<!--Use bootstrap to add links using all 12 columns--> | <!--Use bootstrap to add links using all 12 columns--> | ||
<!--For each link please give double the coloumns for when--> | <!--For each link please give double the coloumns for when--> | ||
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<p id="pp">Here we are submitting KillerRed as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | <p id="pp">Here we are submitting KillerRed as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | ||
− | <p id="pp">The sequence for KillerRed protein coding region can be found here (BBa_K1141002)</p> | + | <p id="pp">The sequence for KillerRed protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914002">(BBa_K1141002)</a></p> |
<h6>Biobrick Code</h6> | <h6>Biobrick Code</h6> | ||
− | <p id="pp">BBa_K1914003</p> | + | <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914003">BBa_K1914003</a></p> |
+ | |||
+ | <br> | ||
+ | <br> | ||
<div class="col-xs-12" style="padding:0;margin:0;"> | <div class="col-xs-12" style="padding:0;margin:0;"> | ||
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<p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | <p id="pp">Here we are submitting KillerOrange as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | ||
− | <p id="pp">The sequence for KillerOrange protein coding region can be found here (BBa_K1914000)</p> | + | <p id="pp">The sequence for KillerOrange protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914000">(BBa_K1914000)</a></p> |
<h6> Biobrick Code </h6> | <h6> Biobrick Code </h6> | ||
− | <p id="pp">BBa_K1914001</p> | + | <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914001">BBa_K1914001</a></p> |
+ | |||
+ | <br> | ||
+ | <br> | ||
<div class="col-xs-12" style="padding:0;margin:0;"> | <div class="col-xs-12" style="padding:0;margin:0;"> | ||
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<p id="pp">Here we are submitting lysozyme as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | <p id="pp">Here we are submitting lysozyme as a composite part under a T7 promoter (BBa_I712074), an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015).</p> | ||
− | <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and exchanged the native signal peptide for one which is <i>E. coli</i> specific and directs Lysozyme to the periplasm. The sequence for Lysozyme protein coding region can be found here (BBa_K1914004)</p> | + | <p id="pp">We have codon optimised the protein coding region, added a FLAG tag and exchanged the native signal peptide for one which is <i>E. coli</i> specific and directs Lysozyme to the periplasm [5]. The sequence for Lysozyme protein coding region can be found here <a href="http://parts.igem.org/Part:BBa_K1914004">(BBa_K1914004)</a></p> |
<h6> Biobrick Code </h6> | <h6> Biobrick Code </h6> | ||
− | <p id="pp">BBa_K1914005</p> | + | <p id="pp"><a href="http://parts.igem.org/Part:BBa_K1914005">BBa_K1914005</a></p> |
+ | |||
+ | <br> | ||
+ | <br> | ||
<div class="col-xs-12" style="padding:0;margin:0;"> | <div class="col-xs-12" style="padding:0;margin:0;"> | ||
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<h6>Description:</h6> | <h6>Description:</h6> | ||
− | <p id="pp"> DNase was designed as a DNA degrading kill switch aiming to prevent cross contamination of DNA</p> | + | <p id="pp"> DNase was designed [6] as a DNA degrading kill switch aiming to prevent cross contamination of DNA</p> |
− | <p id="pp"> | + | <p id="pp"> A composite part containing DNase could not be created. This could be due to the need for a tightly regulating promoter to prevent DNase protein being produced before induction and destroying DNA.</p> |
+ | |||
+ | <br> | ||
+ | <br> | ||
<div class="col-xs-12" style="padding:0;margin:0;"> | <div class="col-xs-12" style="padding:0;margin:0;"> | ||
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</li> | </li> | ||
<li>Blake, C.C.F., Koenig, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., 1965. Structure of hen egg-white lysozyme: a three-dimensional Fourier synthesis at 2 Å resolution. Nature, 206(4986), pp.757-761. | <li>Blake, C.C.F., Koenig, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., 1965. Structure of hen egg-white lysozyme: a three-dimensional Fourier synthesis at 2 Å resolution. Nature, 206(4986), pp.757-761. | ||
+ | </li> | ||
+ | <li>Fischer, B., Perry, B., Phillips, G., Sumner, I. and Goodenough, P., 1993. Physiological consequence of expression of soluble and active hen egg white lysozyme in Escherichia coli. Applied Microbiology and Biotechnology, 39, pp.537–540. | ||
+ | </li> | ||
+ | <li>Chen, C.Y., Lu, S.C. and Liao, T.H.,1998. Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli: purification and characterization of the recombinant enzyme. Gene, 206, pp.181–184. | ||
</li> | </li> | ||
</ol> | </ol> |
Latest revision as of 20:24, 19 October 2016