Difference between revisions of "Team:Alverno CA/Basic Parts"

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{{Alverno_CA}}
 
{{Alverno_CA}}
 
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<script src="http://wayou.github.io/SlipHover/js/jquery.sliphover.min.js">
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    <style type="text/css">
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    #container{
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    width: 750px;
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                text-align:center;
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<head>
 
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<title>Alverno iGEM 2016</title>
 
<title>Alverno iGEM 2016</title>
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<center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
 
<center><img src="https://static.igem.org/mediawiki/2016/thumb/5/58/T--Alverno_CA--Alverno_iGEM_2016_Logo.png/600px-T--Alverno_CA--Alverno_iGEM_2016_Logo.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
 
<h2><center>Basic Parts</center></h2>
 
<h2><center>Basic Parts</center></h2>
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  <div class="demo" id="container">
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        <center>
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            <ul>
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                <li style="list-style: none"><br>
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                <br>
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                <li><img src=
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                "http://placehold.it/215.215"
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                style="height:215px;width:215px;" title=
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                Caption>
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      <li><img src=
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                "http://placehold.it/215.215"
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                Caption>
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                </li>
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                <li><img src=
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                "http://placehold.it/215.215"
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                style="height:215px;width:215px;" title=
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                Caption>
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                </li>
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<br><br><br><br><br><br><br><br><br><br>
  
 
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000:</a></h3></left>
 
<left><h3><a href="http://parts.igem.org/Part:BBa_K2145000">BBa_K2145000:</a></h3></left>
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        $("#menuContainer").css('z-index','99');
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        </script>
 
</html>
 
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Revision as of 20:33, 19 October 2016

Alverno iGEM 2016

Alverno iGEM Logo

Basic Parts













BBa_K2145000:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.

BBa_K2145001:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.

BBa_K2145104:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.

BBa_K2145105:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.

BBa_K2145107:
This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.

BBa_K2145100:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

BBa_K2145101:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

BBa_K2145102:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).

BBa_K2145103:
This plasmid contains a GFP and an RFP reporter separated by a 282-bp spacer containing three gRNA binding sites for dCas. The reporter is designed to demonstrate the effect, if any, of binding a dCas "clamp" between two genes (the idea being that such a clamp can limit propagation of supercoiling between the genes, functionally isolating them).