Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pGFP C120 RFP"

 
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<style>
 
<style>
 +
@media screen and (min-width: 300px) and (max-width: 1000px){
 +
.imageimage1{background-image:url('http://s6.gigacircle.com/media/s6_53fd32d924bc7.gif'); width:100%; height:300px; border-radius:50px; margin-top:100px; overflow:hidden;}
 +
.wordword1{z-index:50; width:100%; height:300px; border-radius:50px; margin-top:130px;}
 +
.wordword1 p{font-size:240px; color:#FFFFFF; /*white-space:pre-wrap;*/  /*word-wrap:break-word;*/ word-spacing:-0.5px;}
 +
.wordword1 h1{font-size:36px; color:#FFFFFF;}
 +
.LBsatisfy{display:none;}
 +
.LBsatisfysp{width:100%; font-size:18px; font-family: 'Product Sans', Arial, sans-serif; margin-bottom:0px;}
 +
.LBsatisfysp h1{height:25px;}
 +
 
.expand{ /*header of 2nd demo*/
 
.expand{ /*header of 2nd demo*/
 
   border-bottom: solid #000000 2px;
 
   border-bottom: solid #000000 2px;
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   padding-bottom: -1000px;
 
   padding-bottom: -1000px;
 
   margin-top: 0px;
 
   margin-top: 0px;
−
   width:60%;
+
   width:80%;
 
   height:40px;
 
   height:40px;
 
   margin:auto;
 
   margin:auto;
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   padding-bottom: 6px !important;
 
   padding-bottom: 6px !important;
 
   margin-top: 29px !important;
 
   margin-top: 29px !important;
−
   width:60%;
+
   width:80%;
 
   margin:auto;
 
   margin:auto;
 
}
 
}
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   padding: 8px;
 
   padding: 8px;
 
   margin: 14px;
 
   margin: 14px;
−
   width: 60%;
+
   width: 80%;
 
   margin-left: auto;
 
   margin-left: auto;
 
   margin-right: auto;
 
   margin-right: auto;
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   text-decoration:none
 
   text-decoration:none
 
}
 
}
−
 
+
.fund a{color:#666; text-decoration:none;}
−
@media screen and (min-width: 300px) and (max-width: 1000px){
+
−
.imageimage1{background-image:url('http://s6.gigacircle.com/media/s6_53fd32d924bc7.gif'); width:100%; height:300px; border-radius:50px; margin-top:100px; overflow:hidden;}
+
−
.wordword1{z-index:50; width:100%; height:300px; border-radius:50px; margin-top:130px;}
+
−
.wordword1 p{font-size:240px; color:#FFFFFF; /*white-space:pre-wrap;*/  /*word-wrap:break-word;*/ word-spacing:-0.5px;}
+
−
.wordword1 h1{font-size:36px; color:#FFFFFF;}
+
−
.LBsatisfy{display:none;}
+
−
.LBsatisfysp{width:100%; font-size:18px; font-family: 'Product Sans', Arial, sans-serif; margin-bottom:0px;}
+
−
.LBsatisfysp h1{height:25px;}
+
 
}
 
}
  
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.LBsatisfy h1{height:35px;}.LBsatisfy h3{color:#FFF; text-indent: 100px;}.LBsatisfy a{color:#FFF; text-decoration:none;}
 
.LBsatisfy h1{height:35px;}.LBsatisfy h3{color:#FFF; text-indent: 100px;}.LBsatisfy a{color:#FFF; text-decoration:none;}
 
.LBsatisfysp{display:none;}
 
.LBsatisfysp{display:none;}
 +
 +
.expand{ /*header of 2nd demo*/
 +
  border-bottom: solid #000000 2px;
 +
  text-shadow: 0px 0px 1px #000000;
 +
  font-size: 24px;
 +
  padding-bottom: -1000px;
 +
  margin-top: 0px;
 +
  width:70%;
 +
  height:40px;
 +
  margin:auto;
 +
}
 +
.technology{ /*header of 2nd demo*/
 +
  cursor: pointer;
 +
  color: #666666 !important;
 +
  border-bottom: solid #000000 2px !important;
 +
  text-shadow: 0px 0px 1px #000000 !important;
 +
  font-size: 24px !important;
 +
  padding-bottom: 6px !important;
 +
  margin-top: 29px !important;
 +
  width:70%;
 +
  margin:auto;
 +
}
 +
div.thelanguage {
 +
  float: none;
 +
  margin-top:47px
 +
  border-radius: 3px;
 +
  padding: 8px;
 +
  margin: 14px;
 +
  width: 70%;
 +
  margin-left: auto;
 +
  margin-right: auto;
 +
  list-style-type: disc;
 +
}
 +
div.thelanguage img {
 +
  width: 50%;
 +
  border: solid rgb(30,123,148) 4px;
 +
  height: 50%;
 +
  margin-bottom: 4px;
 +
  display: block;
 +
}
 +
 +
.openlanguage{ /*class added to contents of 2nd demo when they are open*/
 +
color: #666666;
 +
}
 +
 +
.closedlanguage{ /*class added to contents of 2nd demo when they are closed*/
 +
color: #666666;
 +
}
 +
a.collapseLink {
 +
  color: #666666 !important;
 +
  text-shadow: 0px 0px 1px #000000 !important;
 +
  margin: 12px !important; 
 +
  font-size: 18px !important; 
 +
  text-decoration:none
 +
}
 +
.fund a{color:#666; text-decoration:none;}
 
}
 
}
  
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<body>
 
<body>
 +
 +
    <div class="prototypeprototypesp">
 +
        <div class="imageimage00">
 +
            <img src="https://static.igem.org/mediawiki/2016/1/1c/T--NYMU-Taipei--%E6%96%B0pGFP_C120_RFP.png" width="100%" height="100%" />
 +
        </div>
 +
    </div>
 +
 +
    <div class="prototypeprototype">
 +
        <div class="imageimage00">
 +
            <img src="https://static.igem.org/mediawiki/2016/1/1c/T--NYMU-Taipei--%E6%96%B0pGFP_C120_RFP.png" width="100%" height="100%" />
 +
        </div>
 +
    </div>
  
 
<div id="wrap">
 
<div id="wrap">
 +
<div class="fund">
 +
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-pBARGPE1">pBARGPE1</a></h3></li>
  
−
<div class="LBimageimage1">
+
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-GFP">GFP</a></h3></li>
−
<div class="LBwordword1">
+
  
−
<div class="LBsatisfy">
+
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-TtrpC">TtrpC</a></h3></li>
−
<h1>pGFP_C120_RFP</h1><hr />
+
−
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-pBARGPE1">pBARGPE1</a></h3>
+
−
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-GFP">GFP</a></h3>
+
−
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-TtrpC">TtrpC</a></h3>
+
−
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_C120_empty">C120_empty</a></h3>
+
−
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-minimal-PgpdA">minimal-PgpdA</a></h3>
+
−
<h3><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-RFP">RFP</a></h3>
+
−
</div>
+
−
<div class="LBsatisfysp">
+
−
<h1>pGFP_C120_RFP</h1><hr /></div>
+
  
−
</div></div><br /></div>
+
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-C120_empty">C120_empty</a></h3></li>
 +
 
 +
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-minimal-PgpdA">minimal-PgpdA</a></h3></li>
 +
 
 +
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-RFP">RFP</a></h3></li>
 +
 
 +
</div>
 +
<br />
 +
</div>
  
 
<div class="expand">
 
<div class="expand">
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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
 
style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
−
<div class="technology">8/5</div>
+
<div class="technology">9/23</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px; list-style-type: disc;">pBAR_PgpdA transformed into competent cell</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/6</div>
+
<div class="technology">9/24</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA containing cultures used in 2 in 1</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR</p></li>
 +
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/7</div>
+
<div class="technology">9/30</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA extracted and checked with plasmid PCR and restriction digestion</p></li>
+
<li><p style="font-size: 18px;">Received the junction primer of GFP-TtrpC</p></li>
 +
<li><p style="font-size: 18px;">GFP_TtrpC ran ligation PCR</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/14</div>
+
<div class="technology">10/1</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA plasmid stored and transformed cultures cryopreserved</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC ran ligation PCR</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/18</div>
+
<div class="technology">10/2</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with pVP-EL222 and TtrpC</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC digested for ligation with pBARGPE1</p></li>
 +
<li><p style="font-size: 18px;">Transformed pBAR_GFP_TtrpC into competent cells</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/19</div>
+
<div class="technology">10/3</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC containing cultures used in 2 in 1</p></li>
 +
<li><p style="font-size: 18px;">pExtracted plasmid pBAR_GFP_TtrpC</p></li>
 +
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC digestion check(failure)</p></li>
 +
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC PCR check(failure)</p></li>
 +
<li><p style="font-size: 18px;">Transformed pBAR_GFP_TtrpC into competent cells</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/21</div>
+
<div class="technology">10/4</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC extracted and checked with restriction digestion (failure)</p></li>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC containing cultures used in 2 in 1</p></li>
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC plasmid extraction</p></li>
 +
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC digestion check</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
−
</div>
 
−
 
−
<div class="technology">8/23</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/24</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/28</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------- NC had band</p></li>
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------ with remixed primer</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/29</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------ the concentration was not high enough after gel extraction</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/31</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/1</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/2</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222-TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with EL222_TtrpC</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/3</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with EL222_Ttrp</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 14); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/4</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC extracted from transformed cultures</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 15); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/5</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 16); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/6</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (failure)</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (we changed  enzymes)</p></li>
 
−
<li><p style="font-size: 18px;">EL222-TtrpC digested with restriction enzyme</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 17); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/9</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell( there was no E.coli on our first plate )</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 18); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/10</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 19); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/11</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing culture used in 3 in 1</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 20); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/12</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 
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<li><p style="font-size: 18px;">Successfully constructed the plasmid pBAR_PgpdA_EL222_TtrpC</p></li>
 
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<div class="technology">9/22</div>
 
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<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC digested for transforming into Metarhizium anisopliae</p></li>
 
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Latest revision as of 21:43, 19 October 2016

[Collapse all] | [Expand all]

9/23

  • GFP_TtrpC ligation PCR

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    9/24

  • GFP_TtrpC ligation PCR

  • GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)

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    9/30

  • Received the junction primer of GFP-TtrpC

  • GFP_TtrpC ran ligation PCR

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    10/1

  • GFP_TtrpC ran ligation PCR

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    10/2

  • GFP_TtrpC digested for ligation with pBARGPE1

  • Transformed pBAR_GFP_TtrpC into competent cells

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    10/3

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pExtracted plasmid pBAR_GFP_TtrpC

  • pBAR_GFP_TtrpC digestion check(failure)

  • pBAR_GFP_TtrpC PCR check(failure)

  • Transformed pBAR_GFP_TtrpC into competent cells

    • [Collapse]

    10/4

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pBAR_GFP_TtrpC plasmid extraction

  • pBAR_GFP_TtrpC digestion check

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