Difference between revisions of "Team:UiOslo Norway/Proof"
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| + | <div class="row subsite"> | ||
| + | <div class="col-md-12"> | ||
| + | <div class="row"> | ||
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| + | <div class="col-md-6"> | ||
| + | <div id="proofofConcept"><h2 class="boxheader right"></h2></div> | ||
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| + | <img class="sidephoto" src="https://static.igem.org/mediawiki/2016/a/a8/T--UiOslo_Norway--3dev.jpg"/> | ||
| + | </div> | ||
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| + | <div class="col-md-6"> | ||
| + | <div id="proofofConcept"><h2 class="boxheader right">Proof Of Concept</h2></div> | ||
| + | <p class="boxnotes rightext"> | ||
| + | |||
| + | As a proof of concept, we have successfully demonstrated all the central aspects of our projects with functioning set-ups.</p> | ||
| + | <p class="boxnotes rightext"><b> | ||
| + | Lab: </b><br> | ||
| + | - Our colorimetric assay with nitrocefin is tested and validated, and our detection limits are within a clinically relevant range </p> | ||
| + | <p class="boxnotes rightext"> | ||
| + | - Using BBa_K1189031, a beta lactamase encoding biobrick designed by the Calgary team of 2013, we successfully showed that by using different combinations of inhibitors, we can pin-point the enzymatic class of the beta lactamase present in the samples. | ||
| + | </p> | ||
| + | <p class="boxnotes rightext"> | ||
| + | - By purifying the BBa_K1189031 protein, testing it with nitrocefin and inhibitors and creating and validating our own biobricks for class A, B and C beta lactamases, we have demonstrated safe positive controls to test for these three classes of ESBLs. </p> | ||
| + | <p class="boxnotes rightext"> | ||
| + | <b>Hardware: </b><br> | ||
| + | Our 3D-printed case successfully interfaces urine samples to the phone, illuminates the sample,places and holds the samples on a optimal distance for camera focus. </p> | ||
| + | <p class="boxnotes rightext"> | ||
| + | <b>Software: </b><br><p class="boxnotes rightext"> | ||
| + | We have successfully created an app with an user interface that accesses the camera, reads and analyze the color change induced by the cleavage of nitrocefin from a picture taken in our software. The app also includes an information page where users can read about guidelines regarding UTIs. </p> | ||
| + | <p class="boxnotes rightext"> | ||
| + | Thus, we present a functioning chassis for a mobile diagnostic platform that easily, and within minutes can identify the presence of ESBL producing bacteria in urine samples, without ever having to expose any bacteria to antibiotics. | ||
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| + | </p> | ||
| + | </div> | ||
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| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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| + | <!--- her starter footeren ---> | ||
| + | <div class="row footer"> | ||
| + | <div class="col-md-1 footerelement"></div> | ||
| + | <div class="col-md-7 footerelement"> | ||
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| + | <img src="https://uioslonorway.files.wordpress.com/2016/05/uio_merke_livsvitenskap_life_science_120.png?w=178&h=191" class="sponsoricon rundicon"> | ||
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| + | <img src="https://uioslonorway.files.wordpress.com/2016/05/logo-sarstedt-1.jpg?w=1000" class="sponsoricon"> | ||
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| + | <img src="https://uioslonorway.files.wordpress.com/2016/05/sdl_logo_eng.png?w=1000" class="sponsoricon"> | ||
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| + | <img src="https://uioslonorway.files.wordpress.com/2016/05/last-ned.png?w=417&h=178" class="sponsoricon"> | ||
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| + | <img src="https://uioslonorway.files.wordpress.com/2016/05/nbs.png?w=1000" class="sponsoricon"> | ||
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| + | <img src="https://static.igem.org/mediawiki/2016/a/aa/T--UiOslo_Norway--uio_logo.jpg" class="sponsoricon rundicon"> | ||
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| + | <img src="https://uioslonorway.files.wordpress.com/2016/05/image.png?w=1000" class="sponsoricon"> | ||
| + | |||
| + | |||
| + | </div> | ||
| + | <div class="col-md-4 footerelement"> | ||
| + | <h2 class="boxheader footerheader">Contact</h2> | ||
| + | <p class="boxnotes footercontact"><span class="glyphicon glyphicon-map-marker"></span> Blindernveien 31, 0371 Oslo</p> | ||
| + | <p class="boxnotes footercontact"><span class="glyphicon glyphicon-envelope"></span> uioslonorway@gmail.com</p> | ||
| + | |||
| + | <a class="linkcontact insta" target="_blank" href="https://www.instagram.com/uioslo_igem/"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/9/97/T--UiOslo_Norway--insta_logo.jpg" class="contacticon"> | ||
| + | </a> | ||
| + | |||
| + | <a class="linkcontact" target="_blank" href="https://www.facebook.com/uioslonorwayigem/"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/6/68/T--UiOslo_Norway--face_logo.jpg" class="contacticon"> | ||
| + | </a> | ||
| + | |||
| + | <a class="linkcontact" target="_blank" href="https://uioslonorway.wordpress.com/"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/d/d5/T--UiOslo_Norway--wordpress_logo.jpg" class="contacticon"> | ||
| + | </a> | ||
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| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <!-- Her slutter footeren --> | ||
</html> | </html> | ||
Revision as of 21:50, 19 October 2016
Proof Of Concept
As a proof of concept, we have successfully demonstrated all the central aspects of our projects with functioning set-ups.
Lab:
- Our colorimetric assay with nitrocefin is tested and validated, and our detection limits are within a clinically relevant range
- Using BBa_K1189031, a beta lactamase encoding biobrick designed by the Calgary team of 2013, we successfully showed that by using different combinations of inhibitors, we can pin-point the enzymatic class of the beta lactamase present in the samples.
- By purifying the BBa_K1189031 protein, testing it with nitrocefin and inhibitors and creating and validating our own biobricks for class A, B and C beta lactamases, we have demonstrated safe positive controls to test for these three classes of ESBLs.
Hardware:
Our 3D-printed case successfully interfaces urine samples to the phone, illuminates the sample,places and holds the samples on a optimal distance for camera focus.
Software:
We have successfully created an app with an user interface that accesses the camera, reads and analyze the color change induced by the cleavage of nitrocefin from a picture taken in our software. The app also includes an information page where users can read about guidelines regarding UTIs.
Thus, we present a functioning chassis for a mobile diagnostic platform that easily, and within minutes can identify the presence of ESBL producing bacteria in urine samples, without ever having to expose any bacteria to antibiotics.
Contact
