Difference between revisions of "Team:NRP-UEA-Norwich/Parts"

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All BioBricks consist of basic RBS parts ligated with the pSB1C3 iGEM vector. All BioBricks samples have been tested for accuracy by DNA sequencing. All gene parts in these BioBricks originated from the organism Shewanella oneidensis and were submitted to the iGEM registry on the xx/xx/16 (By Oct 19th DL)
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All BioBricks consist of coding sequences for <i>hydABC</i> and <i>hyaABC</i> subunits for the FeFe & NiFe hydrogenase enzymes from <i>Shewanella oneidensis</i> MR-1. These were ligated into the pSB1C3 iGEM vector. All BioBricks samples have been tested for accuracy by DNA sequencing. All gene parts in these BioBricks originated from the organism <i>Shewanella oneidensis</i> MR-1 and were submitted to the iGEM registry on the 19th of October.
 
</p>
 
</p>
  
 
<table class="tables" >
 
<table class="tables" >
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<thead>
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<tr>
 
<tr>
 
<th>Name in Registry</th>
 
<th>Name in Registry</th>
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<th>Length (bp)</th>
 
<th>Length (bp)</th>
 
</tr>
 
</tr>
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</thead>
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−
<tbody>
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<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957001 BBa_K1957001]</span></td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957001 BBa_K1957001]</span></td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
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<td>Hyd C (Fdh)</td>
+
<td><i>hydC</i> (<i>Fdh</i>)</td>
 
<td>FeFe hydrogenase outer periplasmic subunit</td>
 
<td>FeFe hydrogenase outer periplasmic subunit</td>
−
<td>764</td>
+
<td>705</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957002 BBa_K1957002] </span> </td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957002 BBa_K1957002] </span> </td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
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<td>Hyd B (SII C-terminus)</td>
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<td><i>hydB</i> (SII C-terminus)</td>
 
<td>FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus</td>
 
<td>FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus</td>
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<td>433</td>
+
<td>374</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957003 BBa_K1957003] </span> </td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957003 BBa_K1957003] </span> </td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
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<td>Hyd B (SII N-terminus)</td>
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<td> <i>hydB</i> (SII N-terminus)</td>
 
<td>FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus</td>
 
<td>FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus</td>
−
<td>433</td>
+
<td>374</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957004 BBa_K1957004] </span> </td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957004 BBa_K1957004] </span> </td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
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<td>Hyd A</td>
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<td><i>hydA</i></td>
−
<td>FeFe hydrogenase membrane bound catalytic subunit</td>
+
<td>FeFe hydrogenase membrane bound catalytic subunit</td>
−
<td>1325</td>
+
<td>1266</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957005 BBa_K1957005] </span></td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957005 BBa_K1957005] </span></td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
−
<td>Hya A</td>
+
<td><i>hyaA</i></td>
 
<td>NiFe hydrogenase central subunit</td>
 
<td>NiFe hydrogenase central subunit</td>
−
<td>1215</td>
+
<td>1156</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957006 BBa_K1957006] </span></td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957006 BBa_K1957006] </span></td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
−
<td>Hya C</td>
+
<td><i>hyaC</i></td>
 
<td>NiFe hydrogenase outer periplasmic subunit</td>
 
<td>NiFe hydrogenase outer periplasmic subunit</td>
−
<td>754</td>
+
<td>695</td>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957007 BBa_K1957007] </span></td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957007 BBa_K1957007] </span></td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
−
<td>Hya B (SII C-terminus)</td>
+
<td><i>hyaB</i> (SII C-terminus)</td>
 
<td>NiFe hydrogenase membrane bound catalytic subunit</td>
 
<td>NiFe hydrogenase membrane bound catalytic subunit</td>
−
<td>1830</td>
+
<td>1771</td>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957008 BBa_K1957008] </span></td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957008 BBa_K1957008] </span></td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
−
<td>Hya B (SII N-terminus)</td>
+
<td><i>hyaB</i> (SII N-terminus)</td>
 
<td>NiFe hydrogenase membrane bound catalytic subunit</td>
 
<td>NiFe hydrogenase membrane bound catalytic subunit</td>
−
<td>1830</td>
+
<td>1771</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957009 BBa_K1957009] </span></td>
 
<td><span class="plainlinks">[http://parts.igem.org/Part:BBa_K1957009 BBa_K1957009] </span></td>
 
<td>pSB1C3</td>
 
<td>pSB1C3</td>
−
<td>HydABC, FeFe Hydrogenase gene cluster (SII N-terminus)</td>
+
<td><i>hydABC</i>, FeFe Hydrogenase gene cluster (SII N-terminus)</td>
 
<td>FeFe hydrogenase gene cluster. Strep II tag incorporated onto the N-terminus</td>
 
<td>FeFe hydrogenase gene cluster. Strep II tag incorporated onto the N-terminus</td>
 
<td>2291</td>
 
<td>2291</td>
 
</tr>
 
</tr>
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</tbody>
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</table>
 
</table>
 
</div>
 
</div>
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 +
{{NRP-UEA-Norwich-footer}}

Latest revision as of 21:55, 19 October 2016

NRP-UEA-NORWICH iGEM

Parts

All BioBricks consist of coding sequences for hydABC and hyaABC subunits for the FeFe & NiFe hydrogenase enzymes from Shewanella oneidensis MR-1. These were ligated into the pSB1C3 iGEM vector. All BioBricks samples have been tested for accuracy by DNA sequencing. All gene parts in these BioBricks originated from the organism Shewanella oneidensis MR-1 and were submitted to the iGEM registry on the 19th of October.

Name in Registry Backbone Gene expressed Function Length (bp)
[http://parts.igem.org/Part:BBa_K1957001 BBa_K1957001] pSB1C3 hydC (Fdh) FeFe hydrogenase outer periplasmic subunit 705
[http://parts.igem.org/Part:BBa_K1957002 BBa_K1957002] pSB1C3 hydB (SII C-terminus) FeFe hydrogenase central subunit. Strep II tag incorporated onto C-terminus 374
[http://parts.igem.org/Part:BBa_K1957003 BBa_K1957003] pSB1C3 hydB (SII N-terminus) FeFe hydrogenase central subunit. Strep II tag incorporated onto N-terminus 374
[http://parts.igem.org/Part:BBa_K1957004 BBa_K1957004] pSB1C3 hydA FeFe hydrogenase membrane bound catalytic subunit 1266
[http://parts.igem.org/Part:BBa_K1957005 BBa_K1957005] pSB1C3 hyaA NiFe hydrogenase central subunit 1156
[http://parts.igem.org/Part:BBa_K1957006 BBa_K1957006] pSB1C3 hyaC NiFe hydrogenase outer periplasmic subunit 695
[http://parts.igem.org/Part:BBa_K1957007 BBa_K1957007] pSB1C3 hyaB (SII C-terminus) NiFe hydrogenase membrane bound catalytic subunit 1771
[http://parts.igem.org/Part:BBa_K1957008 BBa_K1957008] pSB1C3 hyaB (SII N-terminus) NiFe hydrogenase membrane bound catalytic subunit 1771
[http://parts.igem.org/Part:BBa_K1957009 BBa_K1957009] pSB1C3 hydABC, FeFe Hydrogenase gene cluster (SII N-terminus) FeFe hydrogenase gene cluster. Strep II tag incorporated onto the N-terminus 2291

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