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<p>The inaugural year of Cardiff iGEM has been an excellent learning experience. There is no doubt that next year we will be in a much stronger position to succeed in all aspects of the our project. We have already set the wheels in motion for a Cardiff_Wales iGEM 2017. | <p>The inaugural year of Cardiff iGEM has been an excellent learning experience. There is no doubt that next year we will be in a much stronger position to succeed in all aspects of the our project. We have already set the wheels in motion for a Cardiff_Wales iGEM 2017. | ||
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<p> <i>Therefore what did we specifically learn from this years iGEM project?</i> | <p> <i>Therefore what did we specifically learn from this years iGEM project?</i> | ||
<li> We would likely use the different pSB1C3 antibiotic resistance variants instead of the three distinct plasmids we chose to use (pSB1C3, pET16B, pCOLA-DUET). This would improve the control of copy number and help maintain uniform expression of our constructs. We were unaware of the different pSB1C3 variants due to our focus on cloning biobricks into the registry-compliant chloramphenicol resistant pSB1C3.</li> | <li> We would likely use the different pSB1C3 antibiotic resistance variants instead of the three distinct plasmids we chose to use (pSB1C3, pET16B, pCOLA-DUET). This would improve the control of copy number and help maintain uniform expression of our constructs. We were unaware of the different pSB1C3 variants due to our focus on cloning biobricks into the registry-compliant chloramphenicol resistant pSB1C3.</li> |
Revision as of 21:59, 19 October 2016
Results
Projects
Cas-Find
Cas-Found
- IPTG inducible CRISPR-Cas9 guide RNA targeted to rRNA Reverse
- Investigated viable systems for future investigation
Can't-Find
- Use of vector (pET16B) with non-expected insert doomed 1/3 of our potential constructs
- Failure to clone Cas-LucC/N & into E.Coli
- As a result we have been unable to study the viablity of our proposed diagnostic tool
FUEL Project
Fuel Up
- We have successfully cloned and sequenced our arabinose-inducible pBAD:mKeima and pBAD:Lux Operon:mKeima constructs
- We have demonstrated that the LuxOperon portion of LUXoperon:mKeima of the fusion construct is functional
Running on Empty
- We have been unable to show a red light output from the mKeima constructs
- We have therefore been unable to observe any red shifting of the light output generated by the Lux Operon
The inaugural year of Cardiff iGEM has been an excellent learning experience. There is no doubt that next year we will be in a much stronger position to succeed in all aspects of the our project. We have already set the wheels in motion for a Cardiff_Wales iGEM 2017.
Therefore what did we specifically learn from this years iGEM project?
Collaborations
Unfortunately we were unable to practically collaborate with Sacley due to failures in our related dCas9. In future years we aim to increase our number of collaborations. We dedicated most of our lab time to cloning our own constructs, which meant there wasn't much time, or manpower left for collaborations.