Difference between revisions of "Team:Aix-Marseille/Design"
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==General objective== | ==General objective== | ||
| − | Nowadays, platinum ressources are an issue because of their planned disappearance toward 2064. Moreover, any alternative has been found so far to counteract the disparition of this metal compared to its central importance in many industries (electronic, automobile, | + | Nowadays, platinum ressources are an issue because of their planned disappearance toward 2064. Moreover, any alternative has been found so far to counteract the disparition of this metal compared to its central importance in many industries (electronic, automobile, medical..) . |
Facing this problem, our goal here is to make a sustainable process, extracting platinum from an other source (road, sludge) than mines ressources, less pollutant, less expensive.. | Facing this problem, our goal here is to make a sustainable process, extracting platinum from an other source (road, sludge) than mines ressources, less pollutant, less expensive.. | ||
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This step aims to : | This step aims to : | ||
* adsorb ions on bacterial flagella protein | * adsorb ions on bacterial flagella protein | ||
| − | * | + | * reduce ions into reduced nanoparticles by the ambient reducer power |
All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinum based on <i>E. coli</i> and <i>Desulfovibrio vulgaris</i> flagellum and synthetic peptides. To this end, we analyze the flagella sequences and structural properties of the external part of the flagella. Then, on the part of the flagellin facing the external medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorption specificity and yield. In this way, peptide would be facing the external medium and being able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promoter enabling a high flagellin production. | All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinum based on <i>E. coli</i> and <i>Desulfovibrio vulgaris</i> flagellum and synthetic peptides. To this end, we analyze the flagella sequences and structural properties of the external part of the flagella. Then, on the part of the flagellin facing the external medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorption specificity and yield. In this way, peptide would be facing the external medium and being able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promoter enabling a high flagellin production. | ||
[[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]] | [[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]] | ||
| − | Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions in the medium and reduce them into | + | Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions in the medium and reduce them into reduced nanoparticules usable in industry. A simple centrifugation of the flagellin binding the platinum allows to concentrate the metal a second time. |
<references/> | <references/> | ||
{{:Team:Aix-Marseille/Template-Footer}} | {{:Team:Aix-Marseille/Template-Footer}} | ||
