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− | + | 2.1.3Enzyme und sonstige Proteine Antikörper Anti-Mouse-Leptin, polyklonaler Antikörper aus Hasen, RD281001220 (BioVendor, Brno,Tschechische Republik) Anti-poly-Histidin, monoklonaler IgG2a Antikörper, Klon HIS-1, HRP konjugiert (Sigma-Aldrich Chemie GmbH, Taufkirchen) Enzyme Lysozym (22770 U/mg) (AppliChem GmbH, Darmstadt) Pfu-Ultra II DNA Polymerase (Promega GmbH, Mannheim) T4-DNA-Ligase (200 CEU/μl) (Fermentas GmbH, St. Leon-Rot) Alkalische Phosphatase aus der arktischen Garnele (1 U/μl)(Thermo Fisher Scientific Inc., St. Leon-Rot) FastAP thermosensitive alkalische Phosphatase (1 U/μl) (Thermo Fisher Scientific Inc., St. Leon-Rot) Restriktionsendonukleasen AfeI / Eco47III (Thermo Fisher Scientific Inc., St. Leon-Rot) HindIII (Thermo Fisher Scientific Inc., St. Leon-Rot) DpnI (Thermo Fisher Scientific Inc., St. Leon-Rot) MlyI (New England Biolabs GmbH, Frankfurt am Main) NcoI (Fermentas GmbH, St. Leon-Rot) NheI (Fermentas GmbH, St. Leon-Rot) SapI (New England Biolabs GmbH, Frankfurt am Main) StuI / Eco147I (Fermentas GmbH, St. Leon-Rot) NgoMIV (New England Biolabs GmbH, Frankfurt am Main) XbaI (Thermo Fisher Scientific Inc., St. Leon-Rot) Sonstige Proteine Rekombinante humane LepR-Fc Chimäre (R&D Systems, Minneapolis, USA) Albumin Fraktion V (BSA) (AppliChem GmbH, Darmstadt) | |
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Revision as of 22:49, 19 October 2016
Materials
2.1.3Enzyme und sonstige Proteine Antikörper Anti-Mouse-Leptin, polyklonaler Antikörper aus Hasen, RD281001220 (BioVendor, Brno,Tschechische Republik) Anti-poly-Histidin, monoklonaler IgG2a Antikörper, Klon HIS-1, HRP konjugiert (Sigma-Aldrich Chemie GmbH, Taufkirchen) Enzyme Lysozym (22770 U/mg) (AppliChem GmbH, Darmstadt) Pfu-Ultra II DNA Polymerase (Promega GmbH, Mannheim) T4-DNA-Ligase (200 CEU/μl) (Fermentas GmbH, St. Leon-Rot) Alkalische Phosphatase aus der arktischen Garnele (1 U/μl)(Thermo Fisher Scientific Inc., St. Leon-Rot) FastAP thermosensitive alkalische Phosphatase (1 U/μl) (Thermo Fisher Scientific Inc., St. Leon-Rot) Restriktionsendonukleasen AfeI / Eco47III (Thermo Fisher Scientific Inc., St. Leon-Rot) HindIII (Thermo Fisher Scientific Inc., St. Leon-Rot) DpnI (Thermo Fisher Scientific Inc., St. Leon-Rot) MlyI (New England Biolabs GmbH, Frankfurt am Main) NcoI (Fermentas GmbH, St. Leon-Rot) NheI (Fermentas GmbH, St. Leon-Rot) SapI (New England Biolabs GmbH, Frankfurt am Main) StuI / Eco147I (Fermentas GmbH, St. Leon-Rot) NgoMIV (New England Biolabs GmbH, Frankfurt am Main) XbaI (Thermo Fisher Scientific Inc., St. Leon-Rot) Sonstige Proteine Rekombinante humane LepR-Fc Chimäre (R&D Systems, Minneapolis, USA) Albumin Fraktion V (BSA) (AppliChem GmbH, Darmstadt)