Difference between revisions of "Team:Nagahama/Description"

 
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{{Nagahama}}
 
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It is a sequel to our theme of the last year.
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__NOTOC__
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<partinfo>BBa_K1230000 short</partinfo>
  
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The MarA protein acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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We used device BBa_K1230004 to characterise this part.
  
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Team iGEM Nagahama 2015
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We improved the characterization of a previously existing BioBrick Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick ''marA'' gene as [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.
  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
 
  
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Team Nagahama 2016
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We improved the characterization of existing this Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000]. We found that marA expression is also improve resistance to farnesol. Result page is [https://2016.igem.org/Team:Nagahama/Result_and_Discussion here]
  
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<h5>What should this page contain?</h5>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<li>References and sources to document your research.</li>
 
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<li>Use illustrations and other visual resources to explain your project.</li>
 
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<!-- Add more about the biology of this part here
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===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1230000 SequenceAndFeatures</partinfo>
  
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<h5>Advice on writing your Project Description</h5>
 
  
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<!-- Uncomment this to enable Functional Parameter display
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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===Functional Parameters===
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<partinfo>BBa_K1230000 parameters</partinfo>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h5>References</h5>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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'''Project description'''
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To innovate a refrigerator without electricity, we invented a prototype of FRAVORATOR that is possible to preserve food by antibacterial volatiles synthesized<br /> by Escherichia coli.
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For high-efficiency synthetic processs, we tried two methods. 1, 1-deoxy-D-xylulose 5-phosphate（DXP）synthase, Dxs, is down-regulated when its downstream<br /> material has been produced to some extent. So, we tried to over-express, another DXP synthase, nDXP, that rarely has been used in normal conditions. 2, Using genomic editing method, we tried to knock out synthase, that we used to produce unnecessary by product.
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Latest revision as of 22:50, 19 October 2016


<partinfo>BBa_K1230000 short</partinfo>

The MarA protein acts as a transcriptional activator in E. coli, it allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to organic solvents, dyes, detergents, antibiotics such as chloramphenicol, tetracycline, novobiocin, erythromycin, fusidic acid and cloxacillin, as well as to cationic antimicrobial peptides, such as LL-37, HNP-2 and HBD-1.

We used device BBa_K1230004 to characterise this part.

Team iGEM Nagahama 2015
We improved the characterization of a previously existing BioBrick Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000] and submitted this improved BioBrick marA gene as [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653006 BBa_K1653006] to iGEM Registry.


Team Nagahama 2016
We improved the characterization of existing this Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000]. We found that marA expression is also improve resistance to farnesol. Result page is here


Sequence and Features <partinfo>BBa_K1230000 SequenceAndFeatures</partinfo>