Difference between revisions of "Team:Aix-Marseille/Safety"

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{{:Team:Aix-Marseille/Template-Top|Safety}}
 
{{:Team:Aix-Marseille/Template-Top|Safety}}
  
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Please visit the [[Safety|main Safety page]] to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.
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==Safe Project Design==
  
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On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)
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During our project the only bacteria we used  were several ''Escherichia coli'' strains that were on the white list. Namely, we used TG1 for the most part of the summer. At the beginning we used DH5 alpha for our cloning experiments but since TG1 strains showed faster and better results we made the switch. We used the W3110 strain for our swimming tests. Finally, we used single-gene knockout strains from the Keio collection for some HPLC and swimming tests.  
  
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===Safe Project Design===
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As for viruses, we only used a P1 bacteriophage for our transduction experiments.
  
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Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:
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==Safe Applications==
  
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* Choosing a non-pathogenic chassis
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When we were researching Scale-up opportunities for our project, we realized that releasing genetically modified bacteria into an open system is reckless and unsafe. Bacteria can easily transfer resistance to antibiotics from one strain to another by horizontal gene transfer or even disturb the local flora and gravely affect the ecosystem. Therefore, our process could only be applied in extremely controlled conditions. Thus, we decided to insert our process into existing [https://2016.igem.org/Team:Aix-Marseille/Integrated_Practices/Environment#Platinum_in_plants roadside vegetation and rainwater treatment systems].
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* Choosing parts that will not harm humans / animals / plants
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* Substituting safer materials for dangerous materials in a proof-of-concept experiment
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* Including an "induced lethality" or "kill-switch" device
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===Safe Lab Work===
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==Safe Lab Work==
  
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Before the beginning of the competition, all of the team members followed a well-being and safety formation with a dedicated member of the Laboratoire d'Ingénierie des Systèmes Macromoléculaires ([http://lism.cnrs-mrs.fr/), Chantal Socia.  
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[[File:T--Aix-Marseille--Safety.jpeg|600px|center|thumb|Our trainer, Chantal Socia, and us]]
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Every day, each member used a waterless hand cleaner before and after experiments. Benches were disinfected with bleach and one bench was dedicated for BET visualization procedures. Lab coat was used every time, gloves were used for molecular biology (e.g., miniprep, [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#PCR PCR]) and biochemestry (e.g., SDS-PAGE), glasses and mask were used for procedure such as HPLC (High Performance Liquid Chromatography) or gel extraction. Biowastes and chemical wastes were sorted in the respect of the lab rules. Laboratory hood were only used for [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Derivatization_reaction HPLC Derivatization reaction step].
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Before the beginning of the competition, all of the team members participated to a safety training with Chantal Socia, a dedicated member of the Laboratoire d'Ingénierie des Systèmes Macromoléculaires [http://lism.cnrs-mrs.fr/ (LISM)].  
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Benches were regularly disinfected with bleach and one bench was dedicated for BET visualization procedures. We always wore lab coats and gloves for molecular biology (e.g., [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.234_:_Plasmid_DNA_purification Plasmid DNA Purification], [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#PCR PCR]) and biochemestry (e.g., [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Electrophoresis Electrophoresis]), glasses and a mask were used for experiments such as [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.239_:_Cadaverin_HPLC_analysis prelimary HPLC protocol] (High Performance Liquid Chromatography) or [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Protocol_.237_:_PCR_clean-up_and_gel_extraction gel extraction]. Biowastes and chemical wastes were sorted according to lab rules. Laboratory hoods were only used for [https://2016.igem.org/Team:Aix-Marseille/Experiments/Protocols#Derivatization_reaction HPLC derivatization reaction step].
  
 
===Safe Shipment===
 
===Safe Shipment===
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Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?
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No safety problems have been encountered in sending DNA parts to the Registry.
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Latest revision as of 23:09, 19 October 2016