Difference between revisions of "Team:IIT-Madras/Composite Part"

(Prototype team page)
 
 
(36 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{IIT-Madras}}
+
{{IIT-Madras-Top/CSS}}
−
<html>
+
= Composite Parts =
 +
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:
 +
1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001], [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002], [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003], [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004].<br>
 +
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing GFP and RFP proteins. Part ranges from [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015], [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018], [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023].<br>
 +
[[Image:Igem_dual_reporter.png | 600x100px | center]]
 +
3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.<br>
 +
[[Image:Iitm_ribos_construct.png | 600x480px | center]]
 +
4.sgRNA parts: We have constructed sgRNA coding parts for mCherry, CamR and KanR genes. There parts are: [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006], [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007], [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008], [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009], [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010], [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011],
 +
[http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012], [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013].
 +
[[Image:Igemiitm_sgRNA.png | 400x60px | center]]
  
  
−
<div class="column full_size judges-will-not-evaluate">
+
= Best Composite Part =
−
<h3>★  ALERT! </h3>
+
Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
+
[[Image:Express_vector.png | 600x60px | center]]
  
−
 
+
{{IIT-Madras-Bottom/CSS}}
−
 
+
−
<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
<div class="column full_size">
+
−
 
+
−
 
+
−
 
+
−
<p>
+
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
−
</p>
+
−
 
+
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
−
 
+
−
 
+
−
 
+
−
<div class="highlight">
+
−
<h4>Note</h4>
+
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
+
−
 
+
−
</div>
+
−
 
+
−
</div>
+
−
 
+
−
</html>
+

Latest revision as of 23:16, 19 October 2016

Composite Parts

We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories: 1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001], [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002], [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003], [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004].
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing GFP and RFP proteins. Part ranges from [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015], [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018], [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023].

Igem dual reporter.png

3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.

Iitm ribos construct.png

4.sgRNA parts: We have constructed sgRNA coding parts for mCherry, CamR and KanR genes. There parts are: [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006], [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007], [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008], [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009], [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010], [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011], [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012], [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013].

Igemiitm sgRNA.png


Best Composite Part

Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.

Express vector.png