Difference between revisions of "Team:IIT-Madras/Composite Part"

 
(16 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{IIT-Madras-Top/CSS}}
 
{{IIT-Madras-Top/CSS}}
 
= Composite Parts =
 
= Composite Parts =
−
<p>We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:</p>
+
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:
−
<p>1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from BBa_K1856001-BBa_1956004.<br>
+
1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001], [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002], [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003], [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004].<br>
−
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing Gfp and Rfp proteins. Part ranges from BBa_K1956014-15, BBa_K1956017-18, BBa_K1956022-23.</p>
+
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing GFP and RFP proteins. Part ranges from [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015], [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018], [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023].<br>
 +
[[Image:Igem_dual_reporter.png | 600x100px | center]]
 +
3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.<br>
 +
[[Image:Iitm_ribos_construct.png | 600x480px | center]]
 +
4.sgRNA parts: We have constructed sgRNA coding parts for mCherry, CamR and KanR genes. There parts are: [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006], [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007], [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008], [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009], [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010], [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011],
 +
[http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012], [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013].
 +
[[Image:Igemiitm_sgRNA.png | 400x60px | center]]
 +
 
  
−
[[Image:Express_vector.png | 800x80px]]
 
−
[[Image:Iitm_ribos_construct.png | 797x105px | center]]
 
 
= Best Composite Part =
 
= Best Composite Part =
−
<p>Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.</p>
+
Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.
−
[[Image:Express_vector.png | 600x60px]]
+
[[Image:Express_vector.png | 600x60px | center]]
  
 
{{IIT-Madras-Bottom/CSS}}
 
{{IIT-Madras-Bottom/CSS}}

Latest revision as of 23:16, 19 October 2016

Composite Parts

We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories: 1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [http://parts.igem.org/Part:BBa_K1956001 BBa_K1956001], [http://parts.igem.org/Part:BBa_K1956002 BBa_K1956002], [http://parts.igem.org/Part:BBa_K1956003 BBa_K1956003], [http://parts.igem.org/Part:BBa_K1956004 BBa_K1956004].
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing GFP and RFP proteins. Part ranges from [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[http://parts.igem.org/Part:BBa_K1956015 BBa_K1956015], [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[http://parts.igem.org/Part:BBa_K1956018 BBa_K1956018], [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[http://parts.igem.org/Part:BBa_K1956023 BBa_K1956023].

Igem dual reporter.png

3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.

Iitm ribos construct.png

4.sgRNA parts: We have constructed sgRNA coding parts for mCherry, CamR and KanR genes. There parts are: [http://parts.igem.org/Part:BBa_K1956006 BBa_K1956006], [http://parts.igem.org/Part:BBa_K1956007 BBa_K1956007], [http://parts.igem.org/Part:BBa_K1956008 BBa_K1956008], [http://parts.igem.org/Part:BBa_K1956009 BBa_K1956009], [http://parts.igem.org/Part:BBa_K1956010 BBa_K1956010], [http://parts.igem.org/Part:BBa_K1956011 BBa_K1956011], [http://parts.igem.org/Part:BBa_K1956012 BBa_K1956012], [http://parts.igem.org/Part:BBa_K1956013 BBa_K1956013].

Igemiitm sgRNA.png


Best Composite Part

Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.

Express vector.png