Difference between revisions of "Team:Aix-Marseille/Medals"

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===Silver medal===
 
===Silver medal===
  
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To compete for the silver medal, we have set up the '''[http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004] functionnal composite biobrick'''. This biobrick were designed from [http://parts.igem.org/Part:BBa_I0500 I0500] (pARA/araC inducible promoter), [http://parts.igem.org/Part:BBa_B0034 B0034] (Ribosome Binding Site) and our designed biobrick  [http://parts.igem.org/Part:BBa_K1951000 K1951000] (Lysine decarboxylase DesA).
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To compete for the silver medal, we have constructed a '''[http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004] functionnal composite biobrick''', that works as expected. This biobrick were designed from [http://parts.igem.org/Part:BBa_I0500 I0500] (pARA/araC inducible promoter), [http://parts.igem.org/Part:BBa_B0034 B0034] (Ribosome Binding Site) and our basic biobrick  [http://parts.igem.org/Part:BBa_K1951000 K1951000] (Lysine decarboxylase DesA).
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To test its functionnality, we have complemented the ''cadA'' mutant with our biobrick [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004]. Results have shown a recovery of the cadaverine biosynthesis and even beyond the rate obtained with the WT strain. To more information, please visit this [http://parts.igem.org/Part:BBa_K1951004:Experience  page].
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To test for function, we have complemented a ''cadA'' mutant with our biobrick [http://parts.igem.org/Part:BBa_K1951004 BBa_K1951004], and demonstrated the recovery of cadaverine biosynthesis. Our results show even more production than obtained with the WT strain. For more information, please visit this [http://parts.igem.org/Part:BBa_K1951004:Experience  page].
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We also helped other iGEM teams:
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* the Bordeaux team by extracting data  from an article and analyze them.
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* the Toulouse team by making a model of plasmid loss.
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All of this can be found [https://2016.igem.org/Team:Aix-Marseille/Collaborations here].
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For our human practices, we present the iGEM competition and the use of the synthetic biology in many [https://2016.igem.org/Team:Aix-Marseille/Engagement events].
  
 
===Gold medal===
 
===Gold medal===
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iGEM 2016 Aix Marseille
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<groupparts>igem2016 Aix-Marseille</groupparts>
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To reach the gold medal, we worked hard to integrate our human practices to our project.
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You can find all this work and how it helped our project [https://2016.igem.org/Team:Aix-Marseille/Integrated_Practices right here].
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We also improved an existing BioBrick, Glasgow 2014 team's BioBrick ([http://parts.igem.org/Part:BBa_K1463604 BBa K1463604]), making our brick ([http://parts.igem.org/Part:BBa_K1951008 BBa K1951008]). This BioBrick is an improvement of the orignial BioBrick in three ways:
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* The codon usage has been optimized to ensure a strong expression in E.coli,
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* The RBS has no mutation so the flagellin is well expressed,
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* Our swimming test show that the swimming phenotype of the BioBick is improved thanks to our changes.
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All the functional tests and experiments, show this part is functional, are listed [https://2016.igem.org/Team:Aix-Marseille/Composite_Part#BBa_K1951008_:_FliC_E._coli_producer here].
  
  
 
{{Aix-Marseille/Footer}}
 
{{Aix-Marseille/Footer}}

Latest revision as of 23:21, 19 October 2016