Difference between revisions of "Team:DTU-Denmark/Engagement"
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<p>“You walk out at the end when you get old, you want to be<br> | <p>“You walk out at the end when you get old, you want to be<br> | ||
able to say, that was worth doing.” </p> | able to say, that was worth doing.” </p> | ||
| − | <small>Randy Rettberg, President of the iGEM-Foundation at<br><i> The European Experience 2016</i | + | <small>Randy Rettberg, President of the iGEM-Foundation at<br><i> The European Experience 2016</i></small> |
</blockquote> | </blockquote> | ||
</div> | </div> | ||
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A day after the transformation is done, the colonies with transformants should be there. It is sometimes necessary to test the biosensor to see if it works as expected, but it depends on the genes in the biosensor. | A day after the transformation is done, the colonies with transformants should be there. It is sometimes necessary to test the biosensor to see if it works as expected, but it depends on the genes in the biosensor. | ||
</p> | </p> | ||
| + | <center> | ||
| + | <!-- image with figure caption and modal that load the same picture --> | ||
| + | <figure class="figure"> | ||
| + | <img id="BSlab" class="enlarge img-responsive figure-img" src="https://static.igem.org/mediawiki/2016/1/1e/T--DTU-Denmark--BiosensorBord.jpg" alt="DESCRIPTION" | ||
| + | style="width:60%"> | ||
| + | <figcaption class="figure-caption">An easy overview of the 3A assembly used for the high school students to create biosensors.</figcaption> | ||
| + | </figure> | ||
| + | </center> | ||
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<h3 class="h3">Security of the Kit</h3> | <h3 class="h3">Security of the Kit</h3> | ||
<p> | <p> | ||
Revision as of 23:24, 19 October 2016
Biosensor
Our human practice strategy is largely build upon a desire to reach beyond the iGEM community and spark an interest of synthetic biology in high school students in Denmark.
Introduction
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The educational platform for 200 high schools in Denmark has now been launched!
Our human practice strategy is largely build upon a desire to reach beyond the iGEM community and spark an interest of synthetic biology in high school students in Denmark.
In Denmark it is not possible for high school students to work with plasmid modification due to controversial rules issued by the Danish Ministry of Education1. As a consequence, very limited knowledge is passed unto the students about synthetic biology and many will only encounter this exciting field when attending university.
We want to change this!
The DTU iGEM team 2015 sketched out the first steps and initiated an exciting project that would allow high school students to work with BioBricks overcoming the Danish rules by using 3A assembly of the parts.
The DTU BioBuilders team 2016 picked up where last year's team let go. With a funding of 300.000 DKK (45.000 USD) from NEB for Biosensor kits and 500.000 DKK (75.000 USD) from Novozymes it was possible to send out kits to the Danish high schools allowing them to create their own customized Biosensors. Elaboration of the project is presented under “Biosensor” beneath.
Project Description
Denmark is a front runner of many innovations. The biotechnology field is no exception and has been offered as a pilot class in Danish high schools since 2008 to get more biological interested students to choose a scientific direction. Even though the pilot scheme has gone well and the trial period has been expanded a couple of times, the law still restricts the possibilities of working with genetically modified organisms. One of the guidelines that the law prescribes is that only plasmids approved by the Danish Ministry for Children, Education and Gender Equality can be used for transformation in Danish high schools and therefore it is time consuming, expensive and difficult for teachers to be innovative in their classes.
New tools that are easy to use and contribute to a high quality level of education in biotechnology are needed to get more students interested in science so Denmark’s position as an innovative country can remain.
The Idea
Last year’s iGEM team from DTU initiated an exciting project that would allow high school students to be innovative in solving national and global problems in medicine, food and environment overcoming the troubles with money, time or the law. The project originates from the BioBrick standard and is called Biosensor. The project is about designing and creating biosensors by choosing a detection gene and a response gene, ligate them and transform it into an approved E. coli strain. The first idea was a simple free exercise for high school students, but the project has developed over the year and now has three elements: a lab kit, a website and competition.
The Biosensor Exercise
The exercise consists of three parts and a test:
- Digestion of selected genes (2 hours)
- Ligation of genes (1 hour)
- Transformation of biosensor (3 hours)
- Test of biosensors
The smart thing about this schedule is that each part takes between 1-3 hours and can be paused by freezing the DNA after each part, so it fits into classes. Examples for biosensors that can be made from the kit:
- A biosensor that can detect when lactose is present and produce lactase to break down the lactose, so lactose intolerant people can drink milk
- A biosensor that can detect light, and when no light is detected produce luciferase that makes light
- Or just a simple biosensor that can detect mercury in cans with tuna and respond by turning red
Like the Danish company LEGO® there are many different combinations and they can solve many different types of problems.
Digestion of Selected Genes
The students select the bricks they want to put in the biosensor and add the restriction enzymes to start the digestion. When the digestion is finished a heat inactivation is required in order to proceed to ligation.
Ligation of Genes
After the inactivation, the genes are ready to be ligated together. The ligation is fast and only takes an hour to complete.
Transformation of Biosensor
The ligated bricks and plasmid are ready to be put together with competent Escherichia coli cells and be transformed by heat shock. The backbone plasmid contains chloramphenicol resistance and the transformants are therefore plated on LB plates with chloramphenicol.
Test of Biosensor
A day after the transformation is done, the colonies with transformants should be there. It is sometimes necessary to test the biosensor to see if it works as expected, but it depends on the genes in the biosensor.
Security of the Kit
The kit is free for all Danish high schools. The protocol has been worked out together with the subject adviser for biotechnology from the Danish Ministry for Children, Education and Gender Equality, Kresten Cæsar Torp and is approved by the ministry and the Danish Working Environment Authority. The result is that all parts are approved and the teachers do not have to apply for each biosensor the students choose to make.
Content of Kit
- Tube with E. coli cells DH10 alpha in 15% glycerol
- Falcon tube
- Inoculation loop
- Shake flask
- Eppendorf tubes
- 25 detection genes
- 25 response genes
- Restriction enzymes
- Labtop cooler
- Primers
- Chemicals
Online Platform
All materials for the exercise is on the website www.biosensor.dk and the students are to submit their experimental results and describe their biosensors, so other students can look at the BioBricks and get inspired. Sharing and looking at other people’s results is a big part of synthetic biology and the students will learn how important it is to make a good characterization and report.
The platform will make it possible for teachers and students to communicate and help each other across the country, and it is especially smart when the kit is updated, so protocols are up to date.
Competition
DTU’s iGEM team will look through the reports and the best biosensors and submit them to the iGEM BioBrick registry. The best biosensor of the year will receive a prize of 5.000 DKK (750 USD). The students also has a possibility of coming up with ideas for a new biosensor. The best idea will also receive a prize of 5.000 DKK (750 USD).
Economy and Funding
A budget for the project in a 3 year period:
| Incomes | 370,000 |
|---|---|
| Sponsorship from Lundbeck Fonden | 70,000 |
| Sponsorship from Novozymes | 300,000 |
| Sponsorship from Otto Mønsted | 100,000 |
| Sponsorship from NovoNordiskfonden | 200,000 |
| Planning expenses | -195,000 |
| Payments | -145,000 |
| Marketing and biosensor camps | -50,000 |
| Expenses related to dispatchment of 200 Biosensor kits | -300,000 |
| 200 Biosensor kits partly sponsored by New England Biolabs | -225,000 |
| Purification of BioBricks by PCR | -35,000 |
| Porto | -30,000 |
| Unforeseen expenditure and reparation of BioBricks | -10,000 |
| Expenses for development of online platform | -254,000 |
| Development of biosensor.dk | -234,000 |
| Maintenance of servers | -20,000 |
| Expenses for yearly Biosensor competition | -50,000 |
| Rewards to students | -45,000 |
| Synthesis of the best biosensors | -5,000 |
| Expenses in total | -799,000 |
Testing at Bagsværd Kostskole
The biosensors have already been tested at our BioBrick Tutorial and after a couple of changes, the biosensors have now been tested for the second time at a local high school. The high schoolers also came with feedback on what they liked and what they thought could be improved. They were thrilled that they could pick the genes they wanted to combine themselves.
