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| <body> | | <body> |
| <header class="header"> | | <header class="header"> |
| + | <div class="navbar-logo"> |
| + | <a class="navbar-brand" href="https://igem.org/Main_Page"> |
| + | <img alt="Brand" src="https://2016.igem.org/wiki/skins/Igem/images/IGEM_white_letters.png"> |
| + | </a> |
| + | </div> |
| + | <div class="navbar-logo"> |
| + | <a class="navbar-brand" href="https://2016.igem.org/Team:HUST-China"> |
| + | <img alt="Brand" src="https://static.igem.org/mediawiki/2016/0/01/T--HUST-China--logo.png" style="left:100px;width:40px;"> |
| + | </a> |
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| + | |
| <nav class="navbar navbar-default"> | | <nav class="navbar navbar-default"> |
− | <div class="container">
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− | <span class="menu"> Menu</span> | + | <span class="menu">Menu</span> |
| <div class="banner-top"> | | <div class="banner-top"> |
| <ul class="nav banner-nav"> | | <ul class="nav banner-nav"> |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown1"><a href="#" class="down-scroll">WETLAB</a> | + | <li class="dropdown1"><a href="#">WETLAB</a> |
| <ul class="dropdown2"> | | <ul class="dropdown2"> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Experiments">Experiments</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Experiments">Experiments</a></li> |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown1"><a class="down-scroll" href="https://2016.igem.org/Team:HUST-China/Model">MODELING</a> | + | <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a> |
| <ul class="dropdown2"> | | <ul class="dropdown2"> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li> |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown1"><a class="down-scroll active" href="">PARTS</a> | + | <li class="dropdown1"><a class="down-scroll active" href="#">PARTS</a> |
| <ul class="dropdown2"> | | <ul class="dropdown2"> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li> |
− | <li><a href="https://2016.igem.org/Team:HUST-China/Basic_Part">Basic part</a></li> | + | <li><a href="https://2016.igem.org/Team:HUST-China/Basic_Part">Basic Part</a></li> |
− | <li><a href="https://2016.igem.org/Team:HUST-China/Composite_Part">Composite part</a></li> | + | <li><a href="https://2016.igem.org/Team:HUST-China/Composite_Part">Composite Part</a></li> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li> |
| </ul> | | </ul> |
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| </ul> | | </ul> |
| </li> | | </li> |
− | <li class="dropdown1"><a class="down-scroll" href="">TEAM</a> | + | <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a> |
| <ul class="dropdown2"> | | <ul class="dropdown2"> |
| <li><a href="https://2016.igem.org/Team:HUST-China/Team">Team Roster</a></li> | | <li><a href="https://2016.igem.org/Team:HUST-China/Team">Team Roster</a></li> |
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| <div class="container"> | | <div class="container"> |
| <h2 class="text-center">Basic Part</h2> | | <h2 class="text-center">Basic Part</h2> |
− | <p style="color:#fff;margin-bottom:10px;text-align:center;">Following documented basic parts are respectively from three circuit this year:</p> | + | <p style="color:#fff;margin-bottom:10px;text-align:center;">Following documented basic parts are respectively from three circuits this year:</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="ref"></div> | | <div class="ref"></div> |
| <article> | | <article> |
− | <h2>Prokaryotic version of Signal Filter:</h2> | + | <h2>Prokaryotic Version of Signal Filter</h2> |
| <img src="https://static.igem.org/mediawiki/2016/8/88/T--HUST-China--Description-Fig-Eukaryote.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/8/88/T--HUST-China--Description-Fig-Eukaryote.png" alt=""> |
− | <h3>Transcriptional regulation:</h3> | + | <h3>Transcriptional Regulation:</h3> |
− | <table border="1"> | + | <table align="center" border="1"> |
| <tr> | | <tr> |
| <td>Part Number</td> | | <td>Part Number</td> |
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| </table> | | </table> |
| <p>Note: We didn’t submit the single parts of pRE, pRM and pR because they are too small to construct, but we have constructed and submitted the composite type of them, using GFP as the report gene in the characterization pathways.</p> | | <p>Note: We didn’t submit the single parts of pRE, pRM and pR because they are too small to construct, but we have constructed and submitted the composite type of them, using GFP as the report gene in the characterization pathways.</p> |
− | <h3>Protein promoter interaction:</h3> | + | <h3>Protein&Promoter Interaction:</h3> |
| <img src="https://static.igem.org/mediawiki/2016/0/06/T--HUST-China--Experiments-Fig10.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/0/06/T--HUST-China--Experiments-Fig10.png" alt=""> |
− | <table border="1"> | + | <table align="center" border="1"> |
| <tr> | | <tr> |
| <td>Part Number</td> | | <td>Part Number</td> |
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| <td><a href="http://parts.igem.org/Part:BBa_K2036032"> BBa_K2036032 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036032"> BBa_K2036032 </a></td> |
| <td>CII-TT-pRE-RBS-GFP-LVAssrAtag</td> | | <td>CII-TT-pRE-RBS-GFP-LVAssrAtag</td> |
− | <td>To characterize CII’s function.</td> | + | <td>To characterize function of CII.</td> |
| </tr> | | </tr> |
| </table> | | </table> |
− | <h3>Protein&protein interaction:</h3> | + | <h3>Protein&Protein Interaction:</h3> |
| <img src="https://static.igem.org/mediawiki/2016/f/f9/T--HUST-China--Experiments-Fig6.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/f/f9/T--HUST-China--Experiments-Fig6.png" alt=""> |
− | <table border="1"> | + | <table align="center" border="1"> |
| <tr> | | <tr> |
| <td>Part Number</td> | | <td>Part Number</td> |
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| </tr> | | </tr> |
| </table> | | </table> |
− | <p>Note: CIII and CII circuits above share the same assembly composition of pRE-RBS-GFP-LVAssrAtag with CII and pRE characterization circuit so the part is not listed repeatedly.</p> | + | <p>Note: CIII and CII circuit above shares the same assembly composition of pRE-RBS-GFP-LVAssrAtag with CII and pRE characterization circuit so the part is not listed repeatedly.</p> |
− | <h3>Tri-stable function:</h3> | + | <h3>Tri-stable Function:</h3> |
| <img src="https://static.igem.org/mediawiki/2016/1/17/T--HUST-China--Experiments-Fig14.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/1/17/T--HUST-China--Experiments-Fig14.png" alt=""> |
− | <table border="1"> | + | <table align="center" border="1"> |
| <tr> | | <tr> |
| <td>Part Number</td> | | <td>Part Number</td> |
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| <td><a href="http://parts.igem.org/Part:BBa_K2036018"> BBa_K2036018 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036018"> BBa_K2036018 </a></td> |
| <td> pRE-RBS-Cro-RBS-CII-TT-ptrp </td> | | <td> pRE-RBS-Cro-RBS-CII-TT-ptrp </td> |
− | <td> Used to construct the circuit. A part of the positive feedback system.</td> | + | <td> Used to construct the circuit. A part of the positive-feedback system.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td><a href="http://parts.igem.org/Part:BBa_K2036019"> BBa_K2036019 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036019"> BBa_K2036019 </a></td> |
− | <td> RFP-LVAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td> | + | <td> RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td> |
| <td> The report part of the circuit.RFP is used as gene of interest 1 and GFP as gene of interest 2.</td> | | <td> The report part of the circuit.RFP is used as gene of interest 1 and GFP as gene of interest 2.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td><a href="http://parts.igem.org/Part:BBa_K2036020"> BBa_K2036020 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036020"> BBa_K2036020 </a></td> |
− | <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LVAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td> | + | <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td> |
| <td> Used to construct the circuit.</td> | | <td> Used to construct the circuit.</td> |
| </tr> | | </tr> |
| </table> | | </table> |
− | <h2>Eukaryotic version of Signal Filter:</h2> | + | <h2>Eukaryotic Version of Signal Filter</h2> |
− | <h3>Functional protein and promoter:</h3> | + | <h3>Functional Protein and Promoter:</h3> |
| <img src="https://static.igem.org/mediawiki/2016/1/1e/T--HUST-China--Description-Fig-prokaryote.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/1/1e/T--HUST-China--Description-Fig-prokaryote.png" alt=""> |
− | <table border="1"> | + | <table align="center" border="1"> |
| <tr> | | <tr> |
| <td>Part Number</td> | | <td>Part Number</td> |
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| <td><a href="http://parts.igem.org/Part:BBa_K2036004"> BBa_K2036004 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036004"> BBa_K2036004 </a></td> |
| <td> ABF2</td> | | <td> ABF2</td> |
− | <td> A leucine zipper transcription factor which can activate pRD29A.</td> | + | <td> A leucine zipper transcription factor which can activate Prd29A.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td><a href="http://parts.igem.org/Part:BBa_K2036003"> BBa_K2036003 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036003"> BBa_K2036003 </a></td> |
| <td> SnRK2.2</td> | | <td> SnRK2.2</td> |
− | <td> Sucrose Nonfermenting1-Related Subfamily2(SnRK2s) protein kinase which can phosphorylate ABF2.</td> | + | <td> Sucrose Nonfermenting1 Related Subfamily2 (SnRK2s) protein kinase which can phosphorylate ABF2.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td><a href="http://parts.igem.org/Part:BBa_K2036002"> BBa_K2036002 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036002"> BBa_K2036002 </a></td> |
− | <td> prd29A </td> | + | <td> Prd29A </td> |
− | <td> A promoter in the Abscisic acid signaling network which can be activated by ABF2.</td> | + | <td> A promoter in the Abscisic Acid signaling network which can be activated by ABF2.</td> |
| </tr> | | </tr> |
| </table> | | </table> |
− | <h3>Bi-stable-function</h3> | + | <h3>Bi-stable Function</h3> |
| <img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt="" class="half_width"><img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt="" class="half_width"> | | <img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt="" class="half_width"><img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt="" class="half_width"> |
− | <table border="1"> | + | <table align="center" border="1"> |
| <tr> | | <tr> |
| <td>Part Number</td> | | <td>Part Number</td> |
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| <tr> | | <tr> |
| <td><a href="http://parts.igem.org/Part:BBa_K2036029"> BBa_K2036029 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036029"> BBa_K2036029 </a></td> |
− | <td> Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag </td> | + | <td> Kozak-ABF2-TTADH1-Prd29A-Kozak-GFP-LVAssrAtag </td> |
| <td> The report part of the circuit.GFP is used as gene of interest.</td> | | <td> The report part of the circuit.GFP is used as gene of interest.</td> |
| </tr> | | </tr> |
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| </tr> | | </tr> |
| </table> | | </table> |
− | <h2>Application of Signal Filter:</h2> | + | <h2>Application of Signal Filter</h2> |
| <img src="https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--modeling-application.png" alt=""> | | <img src="https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--modeling-application.png" alt=""> |
− | <table border="1"> | + | <table align="center" border="1"> |
| <tr> | | <tr> |
| <td>Part Number</td> | | <td>Part Number</td> |
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| <td><a href="http://parts.igem.org/Part:BBa_K2036021"> BBa_K2036021 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036021"> BBa_K2036021 </a></td> |
| <td> placm-pRE-RBS-Cro-RBS-CII-TT </td> | | <td> placm-pRE-RBS-Cro-RBS-CII-TT </td> |
− | <td> Used to construct the circuit. A part of the positive feedback system. placm can response to lactose signal.</td> | + | <td> Used to construct the circuit. A part of the positive-feedback system. placm can respond to lactose signal.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td><a href="http://parts.igem.org/Part:BBa_K2036022"> BBa_K2036022 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036022"> BBa_K2036022 </a></td> |
| <td> patp2-RBS-CI-TT-pR-RBS-CIII </td> | | <td> patp2-RBS-CI-TT-pR-RBS-CIII </td> |
− | <td> Used to construct the circuit. Patp2 can response to base signal.</td> | + | <td> Used to construct the circuit. Patp2 can respond to base signal.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
| <td><a href="http://parts.igem.org/Part:BBa_K2036023"> BBa_K2036023 </a></td> | | <td><a href="http://parts.igem.org/Part:BBa_K2036023"> BBa_K2036023 </a></td> |
| <td> RBS-iLDH-TT-pRM-RBS-beta-galactosidase </td> | | <td> RBS-iLDH-TT-pRM-RBS-beta-galactosidase </td> |
− | <td> The report part of the application circuit.iLDH is used as gene of interest 1 and beta-galactosidase the gene of interest 2.</td> | + | <td> The report part of the application circuit.iLDH is used as gene of interest 1 and beta-galactosidase as gene of interest 2.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |