Difference between revisions of "Team:Pasteur Paris/Protocol"
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<td><strong>Microbiology equipment</strong></td> | <td><strong>Microbiology equipment</strong></td> | ||
<td>Materials always used in the lab </td> | <td>Materials always used in the lab </td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/40/T--Pasteur_Paris--equipment.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/40/T--Pasteur_Paris--equipment.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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<td><strong><p>PCR</p></strong></td> | <td><strong><p>PCR</p></strong></td> | ||
<td>To reproduce (amplify) selected sections of DNA (inserts A1, A2, B1, B2, C1, C2, E1,E2).</td> | <td>To reproduce (amplify) selected sections of DNA (inserts A1, A2, B1, B2, C1, C2, E1,E2).</td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/b/be/T--Pasteur_Paris--PCR_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/b/be/T--Pasteur_Paris--PCR_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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<td><strong>Digestion</strong></td> | <td><strong>Digestion</strong></td> | ||
<td>To linearize the different plasmids with appropriate enzymes. Perform restriction enzyme digestion in order to recover linear backbones of the plasmids. And chose the appropriate restriction sites based on the host plasmids. </td> | <td>To linearize the different plasmids with appropriate enzymes. Perform restriction enzyme digestion in order to recover linear backbones of the plasmids. And chose the appropriate restriction sites based on the host plasmids. </td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/a/ab/T--Pasteur_Paris--Restriction_digestion_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/a/ab/T--Pasteur_Paris--Restriction_digestion_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>Dephosphorylation</strong></td> | <td><strong>Dephosphorylation</strong></td> | ||
<td>To reduce vector self-ligation and favor that of vector to insert instead. </td> | <td>To reduce vector self-ligation and favor that of vector to insert instead. </td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/c/c0/T--Pasteur_Paris--dephosphorylation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/c/c0/T--Pasteur_Paris--dephosphorylation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>To link an insert to its host plasmid before the transformation. This step happens after the digestion of the insert and the plasmid with the same restriction sites enzymes. | <td>To link an insert to its host plasmid before the transformation. This step happens after the digestion of the insert and the plasmid with the same restriction sites enzymes. | ||
</td> | </td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/6/6f/T--Pasteur_Paris--Ligation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/6/6f/T--Pasteur_Paris--Ligation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>Electrophoresis</strong></td> | <td><strong>Electrophoresis</strong></td> | ||
<td>To know the size of DNA fragments to check the efficiency of a digestion for instance.</td> | <td>To know the size of DNA fragments to check the efficiency of a digestion for instance.</td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/6/60/T--Pasteur_Paris--Gel_electrophoresis_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/6/60/T--Pasteur_Paris--Gel_electrophoresis_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>Gel extraction kit</strong></td> | <td><strong>Gel extraction kit</strong></td> | ||
<td>To get back the DNA purified thanks to the electrophoresis on agarose gel.</td> | <td>To get back the DNA purified thanks to the electrophoresis on agarose gel.</td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4e/T--Pasteur_Paris--Gel_extraction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4e/T--Pasteur_Paris--Gel_extraction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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<td><strong>Miniprep QIAGEN Kit</strong></td> | <td><strong>Miniprep QIAGEN Kit</strong></td> | ||
<td> To extract DNA from a culture of bacteria</td> | <td> To extract DNA from a culture of bacteria</td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/d/d5/T--Pasteur_Paris--Miniprep_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/d/d5/T--Pasteur_Paris--Miniprep_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>Harvest and culture</strong></td> | <td><strong>Harvest and culture</strong></td> | ||
<td>In order to obtain a large amount of plasmid, we need to grow the bacteria overnight.</td> | <td>In order to obtain a large amount of plasmid, we need to grow the bacteria overnight.</td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4b/T--Pasteur_Paris--Bacterial_culture_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4b/T--Pasteur_Paris--Bacterial_culture_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>Stab culture</strong></td> | <td><strong>Stab culture</strong></td> | ||
<td>To store a clone of bacteria to be used later.</td> | <td>To store a clone of bacteria to be used later.</td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/b/b1/Stab_culture_Pasteur_Paris_2016.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/b/b1/Stab_culture_Pasteur_Paris_2016.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>IPTG induction</strong></td> | <td><strong>IPTG induction</strong></td> | ||
<td>To increase the production of our protein which depends on this molecule.</td> | <td>To increase the production of our protein which depends on this molecule.</td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/a/a4/T--Pasteur_Paris--Protein_induction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/a/a4/T--Pasteur_Paris--Protein_induction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>Protein Purification Fast Protein Liquid Chromatography</strong></td> | <td><strong>Protein Purification Fast Protein Liquid Chromatography</strong></td> | ||
<td>To check if the His-tag works and if our protein has really been produced </td> | <td>To check if the His-tag works and if our protein has really been produced </td> | ||
| − | <td> <center><a href="https://static.igem.org/mediawiki/2016/0/07/T--Pasteur_Paris--FPLC_Protein_purification_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/ | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/0/07/T--Pasteur_Paris--FPLC_Protein_purification_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--Pasteur_Paris--DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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